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Plasmid#65500PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-t7CFPt9-cMycExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only
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pFLIP39
Plasmid#65507PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-edAFPt9-attR1 and attR2-t7edCFPt9-cMycExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFLIP34
Plasmid#65501PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-t7TFPt9-cMycExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFLIP35
Plasmid#65502PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-TFPt9-cMycExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFLIP36
Plasmid#65504PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-Cer-cMycExpressionMutationPromoterT7 promoterAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFLIP42
Plasmid#65617PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-Cit-attR1 and attR2-mCer-cMycExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
His6-PRC1
Plasmid#69111PurposeFor bacterial expression of recombinant human PRC1 tagged with His.DepositorInsertProtein Regulator of Cytokinesis 1 (PRC1 Human)
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceSept. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJB01
Plasmid#111957PurposeExpresses His10-RFP-Halo; construct generated for pull-down and localization experimentsDepositorInsertRFP
UseTagsHaloTagExpressionBacterialMutationPromoterT7Available sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
KaiC CI cat-/EE (E77Q; E78Q; S431E; T432E)
Plasmid#41888DepositorInsertKai C CI cat-/EE
UseTagsExpressionBacterialMutationchanged amin acids: 77,78,431 and 432 to glutamatePromotert7Available sinceMarch 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
KaiC CII cat-/EE (E318Q; S431E; T432E)
Plasmid#42498DepositorInsertKaiC CII cat-/EE (E318Q; S431E; T432E)
UseTagsExpressionBacterialMutationE318Q; S431E; T432EPromotert7Available sinceMarch 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
KaiC CI cat- (E77Q;E78Q)
Plasmid#41886DepositorInsertKaiC CI cat -(E77Q;E78Q)
UseTagsExpressionBacterialMutationchanged glutamic acid 77 and 78 to glutaminePromotert7 promoterAvailable sinceMarch 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
KaiC CII cat- (E318Q)
Plasmid#41887DepositorInsertKai C CII cat (E318Q)
UseBl de3 21TagsExpressionBacterialMutationchanged amino acid 318 E to QPromotert7Available sinceMarch 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pALOD4 non-binding mutant
Plasmid#111027PurposeExpresses non-binding mutant version of p-ALOD4, has six inactivating mutations (G501A, T502A, T503A, L504A, Y505A, and P506A)DepositorInsertDomain 4 of Anthrolysin O
UseTagsHis6 tagExpressionBacterialMutationMutations: S404C, C472A, G501A, T502A, T503A, L5…PromoterT7Available sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
T7-His-GFP-Arp11
Plasmid#51402Purposeexpression of GFP-tagged mouse Arp11 under T7 promoterDepositorInsertArp11 (Actr10 Mouse)
UseTagsGFP and HisExpressionBacterialMutationPromoterT7Available sinceMarch 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
T7-His-Arp11(1–417)
Plasmid#51400Purposeexpression of full-length mouse Arp11 under T7 promoterDepositorInsertArp11 (Actr10 Mouse)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceFeb. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPI486
Plasmid#207441Purpose6xHis-SS-TEV-ErkB bacterial expression vectorDepositorInsertErkB
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPI580
Plasmid#207445Purpose6xHis-SS-TEV-ErkA bacterial expression vectorDepositorInsertErkA
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPI544
Plasmid#207443Purpose6xHis-SS-TEV-ErkB-ErkB-T176A bacterial expression vectorDepositorInsertErkB
UseUnspecifiedTagsExpressionMutationT176APromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPI560
Plasmid#207444Purpose6xHis-SS-TEV-ErkB-Y178A bacterial expression vectorDepositorInsertErkB
UseUnspecifiedTagsExpressionMutationY178APromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGP-RSET-iGABASnFR2-6xHis
Plasmid#218885PurposeBacterial expression of improved GABA sensor (positive change in fluorescence)DepositorInsertiGABASnFR2
UseTags6xHisExpressionBacterialMutationS99A F102Y F104Y L178SPromoterT7Available sinceMay 14, 2024AvailabilityAcademic Institutions and Nonprofits only