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pHLuc Control

Plasmid

#141091

Purpose

pH-stable bioluminescent reporter that serves as the control counterpart of pHLuc; utilizes eGFP instead of SEP

Depositor

Jeak Ling Ding

Article

Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

Insert

Antares-T2A-puromycin-IRESv24-eGFP-Nanoluc

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 11, 2020

Availability

Academic Institutions and Nonprofits only

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SMN2E7-Fluc-Nluc-reporter

Plasmid

#221005

Purpose

Transiently express SMN2 dual luciferase reporter. Inclusion of E7 produces in-frame stop codon and Firefly luciferase only, while skipping produces both Firefly and Nanoluc. CMV promoter.

Depositor

Benjamin Blencowe, Mikko Taipale

Article

Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

Insert

Fluc-SMN2E7-STOP-Nluc

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pNLuc

Plasmid

#118058

Purpose

GBPart - NLuc Luciferase CDS

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

Nano Luciferase

Use

Luciferase and Synthetic Biology; Gbpart

Tags

Expression

Mutation

Promoter

Available since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pET20b-NLuc-MBP-6xHis

Plasmid

#135931

Purpose

Expresses NanoLuciferase in bacterial cells.

Depositor

Antonio Amelio

Article

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

NanoLuciferase

Use

Tags

MBP-6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-S-Luc_cyto

Plasmid

#113926

Purpose

Plasmid encoding the cytosolic localization of the chimeric SNAP-NanoLuc fusion S-Luc in mammalian cells

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

S-Luc

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-T2A-Nluc-3xP3-RFP

Plasmid

#127563

Purpose

CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Nluc-SV40 and 3xP3-RFP visible eye marker.

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

Nluc

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-S-Luc_mito

Plasmid

#113927

Purpose

Plasmid encoding the mitochondrial localization of the chimeric SNAP-NanoLuc fusion S-Luc in mammalian cells

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

S-Luc_mito

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_S-Luc_CLIP

Plasmid

#113923

Purpose

Plasmid encoding the bacterial expression of the chimeric SNAP-NanoLuc fusion dubbed S-Luc c-terminally linked to CLIP-tag

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

S-Luc_CLIP

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-S-Luc_nucl

Plasmid

#113928

Purpose

Plasmid encoding the nuclear localization of the chimeric SNAP-NanoLuc fusion S-Luc in mammalian cells

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

S-Luc_nucl

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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bdSUMO-GFP-HiBit

Plasmid

#170989

Purpose

For bacterial expression of bdSUMO tagged muGFP with NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-muGFP-HiBiT

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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bdSUMO-E5TAT-GFP-HiBit

Plasmid

#170993

Purpose

For bacterial expression of bdSUMO tagged muGFP with E5TAT peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-E5TAT-muGFP-HiBiT

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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bdSUMO-TAT-GFP-HiBit

Plasmid

#170991

Purpose

For bacterial expression of bdSUMO tagged muGFP with TAT peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-TAT-muGFP-HiBiT

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pEF1a-2xSV40_NLS-NLuc

Plasmid

#135953

Purpose

Expresses the NanoLuciferase targeted to the nucleus in mammalian cells.

Depositor

Antonio Amelio

Article

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

NanoLuciferase

Use

Tags

2xSV40-NLS

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pVH617

Plasmid

#169606

Purpose

Tier-2 vector encoding PmPGK1-driven Fluc and PCREm4-driven Nluc expression (A1-pA::PmPGK1-Fluc-pA::PCREm4-Nluc-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven FLuc Luciferase expression cassette and cAMP response element-controlled NanoLuc Luciferase expression cassette

Use

Tags

Expression

Mammalian

Mutation

Promoter

PCREm4 / PPGK

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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bdSUMO-HA2-GFP-HiBit

Plasmid

#171017

Purpose

For bacterial expression of bdSUMO tagged muGFP with HA2 peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-HA2-muGFP-HiBiT

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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bdSUMO-ZF5.3-GFP-HiBit

Plasmid

#170992

Purpose

For bacterial expression of bdSUMO tagged muGFP with ZF5.3 peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-muGFP-HiBiT

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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bdSUMO-R9-GFP-HiBit

Plasmid

#170990

Purpose

For bacterial expression of bdSUMO tagged muGFP with R9 peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-R9-muGFP-HiBiT

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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bdSUMO-pHD118-GFP-HiBit

Plasmid

#171015

Purpose

For bacterial expression of bdSUMO tagged muGFP with pHD118 peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-pHD118-muGFP-HiBiT

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pTS2311-Tier1-spacer-PPGK-NLuc_TB

Plasmid

#169552

Purpose

Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 preluded by a 5' spacer sequence (spacer-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-NanoLuc Luciferase - mTagBFP fusion

Use

Tags

Expression

Mammalian

Mutation

Promoter

PPGK

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pBTK206

Plasmid

#110588

Purpose

BTK Type 3 coding sequence for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

Nanoluc

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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