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Plasmid#119989PurposeTemplate for C-terminal PCR tagging of mammalian genes (without selection) with mKOkDepositorInsertmKOk
UsePcr templateTagsmKOkExpressionMutationPromoterNoneAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
optGluCl alpha EYFP
Plasmid#15104DepositorInsertGluCl alpha (glc-1 Nematode)
UseTagsEYFPExpressionMammalianMutationCodon optimized cDNAPromoterAvailable sinceJune 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
optGluCl beta Y182F ECFP
Plasmid#15105DepositorInsertGluCl beta (glc-2 Nematode)
UseTagsECFPExpressionMammalianMutationCodon optimized cDNA, Y182FPromoterAvailable sinceJune 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P10
Plasmid#120021PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mKOkDepositorInsertmKOk
UsePcr templateTagsmKOkExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z10
Plasmid#120053PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mKOkDepositorInsertmKOk
UsePcr templateTagsmKOkExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-rap1-K9R
Plasmid#84527PurposeThis plasmid can be used as a donor for subcloning into Gateway destination vectors.DepositorInsertRAP1 (RAP1 Budding Yeast)
UseGateway donor plasmidTagsExpressionMutationK(43,61,69,117,240,246,527,582,651)RPromoternoneAvailable sinceJan. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-RAP1
Plasmid#84525PurposeThis plasmid can be used as a donor for subcloning into Gateway destination vectors.DepositorInsertRAP1 (RAP1 Budding Yeast)
UseGateway donor plasmidTagsExpressionMutationno stop codonPromoternoneAvailable sinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-rap1-K651R
Plasmid#84528PurposeThis plasmid can be used as a donor for subcloning into Gateway destination vectors.DepositorInsertRAP1 (RAP1 Budding Yeast)
UseGateway donor plasmidTagsExpressionMutationK651RPromoternoneAvailable sinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG415-ADH1p-HA-rap1-K9R
Plasmid#84531PurposeThis plasmid can be used to express HA-tagged Rap1-K9R from the ADH promoter in yeastDepositorInsertRAP1 (RAP1 Budding Yeast)
UseTagsHAExpressionYeastMutationK(43,61,69,117,240,246,527,582,651)RPromoterADH1Available sinceJan. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAG415-GPDp-EGFP-rap1-K3NR
Plasmid#84533PurposeThis plasmid can be used to express EGFP-tagged Rap1K3NR from the GDP promoter in yeastDepositorInsertRAP1 (RAP1 Budding Yeast)
UseTagsEGFPExpressionYeastMutationK(43,117,118)RPromoterGPDAvailable sinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG415-GPDp-EGFP-rap1-K3CR
Plasmid#84534PurposeThis plasmid can be used to express EGFP-tagged Rap1K3CR from the GDP promoter in yeastDepositorInsertRAP1 (RAP1 Budding Yeast)
UseTagsEGFPExpressionYeastMutationK(462,463,651)RPromoterGPDAvailable sinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG415-GPDp-EGFP-rap1-K9R
Plasmid#84535PurposeThis plasmid can be used to express EGFP-tagged Rap1K9R from the GDP promoter in yeastDepositorInsertRAP1 (RAP1 Budding Yeast)
UseTagsEGFPExpressionYeastMutationK(43,61,69,117,240,246,527,582,651)RPromoterGPDAvailable sinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-rap1-K462-463-651R
Plasmid#84564PurposeThis plasmid can be used as a donor for subcloning into Gateway destination vectors.DepositorInsertRAP1 (RAP1 Budding Yeast)
UseGateway donor plasmidTagsExpressionMutationK462-463-651RPromoternoneAvailable sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-rap1-K43-117-118R
Plasmid#84565PurposeThis plasmid can be used as a donor for subcloning into Gateway destination vectors.DepositorInsertRAP1 (RAP1 Budding Yeast)
UseGateway donor plasmidTagsExpressionMutationK43-117-118RPromoternoneAvailable sinceOct. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG415-GPDp-EGFP-rap1-K651R
Plasmid#84536PurposeThis plasmid can be used to express EGFP-tagged Rap1-K651R from the GDP promoter in yeastDepositorInsertRAP1 (RAP1 Budding Yeast)
UseTagsEGFPExpressionYeastMutationK651RPromoterGPDAvailable sinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
FRP2008
Plasmid#159312PurposeYeast tagging vector to create gene fusion with yeast codon optimized mKOkappa under KanMX selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationV2delPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMMK801
Plasmid#207140PurposeType 8 Tn10 backbone part, with RFP dropoutDepositorTypeEmpty backboneUseTagsExpressionMutationPromotern/aAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJKW0957
Plasmid#119026PurposeE.coli expression plasmid for PmKOMT1DepositorInsertPmKOMT1
UseTags8xHisExpressionBacterialMutationPromoterT7Available sinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJKW0826
Plasmid#119027PurposeE.coli expression plasmid for PmKOMT2DepositorInsertPmKOMT2
UseTags8xHisExpressionBacterialMutationPromoterT7Available sinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTU-A-005
Plasmid#124412PurposeSlot A, contains mKO2-MarA (step 1)DepositorInsertslot A with mKO2-MarA reporter + terminator + spacer
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 12, 2019AvailabilityAcademic Institutions and Nonprofits only