-
Plasmid#217300PurposeLentivirus vector for expression of MEK1 K57NDepositorInsertMAP2K1 (MAP2K1 Human)
UseLentiviralTagsV5ExpressionMutationK57NPromoterAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
MEK1-Q56P-V5
Plasmid#217299PurposeLentivirus vector for expression of MEK1 Q56PDepositorInsertMAP2K1 (MAP2K1 Human)
UseLentiviralTagsV5ExpressionMutationQ56PPromoterAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-Puro
Plasmid#52963PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.DepositorHas ServiceCloning Grade DNAInsertsS. pyogenes sgRNA cassette
Puromycin resistance
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEf1-a and hU6Available sinceMay 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
CMV-loxp-DsRed-loxp-eGFP
Plasmid#141148Purposelentiviral expression vector to generate a hypoxia fate-mapping systemDepositorInsertloxp-DsRed-loxp-eGFP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLIK_hygro_3xflag_Luciferase
Plasmid#136528PurposeLentiviral expression vector for an inducible 3xflag-tagged LuciferaseDepositorInsertLuciferase
UseLentiviral; Destinatioin vector for gateway cloni…Tags3x FLAGExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCEF-VSV-G
Plasmid#41792PurposeVSV-G-expressing envelope plasmidDepositorInsertVesicular stomatitis virus G glycoprotein, Indiana strain
UseTagsExpressionMammalianMutationPromoterCEFAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLIK_Zeo_3xflag_Luciferase
Plasmid#136533PurposeLentiviral expression vector for an inducible 3xflag-tagged LuciferaseDepositorInsertLuciferase
UseLentiviral; Destinatioin vector for gateway cloni…Tags3x FLAGExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lenti-entry-puro
Plasmid#85745PurposeLentiviral vector for generating multiple sgRNAs carrying plasmids by Golden Gate Assembly.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-FuG-B2
Plasmid#67513PurposeThis is an envelope gene encoding plasmid to make retrogradely lentiviral vector. You can make type B2 envelope coating virus particle with HiRet by using this plasmid.DepositorInsertFusion Glycoprotein type B2
UseLentiviralTagsFusion protein of Vesicular stomatitis Indiana vi…ExpressionMutationPromoterCAGGSAvailable sinceJuly 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
MEK1-DD-V5
Plasmid#217298PurposeLentivirus vector for expression of MEK1 S218D/S222DDepositorInsertMAP2K1 (MAP2K1 Human)
UseLentiviralTagsV5ExpressionMutationS218D/S222DPromoterAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
MEK1-E102-I103del-V5
Plasmid#217301PurposeLentivirus vector for expression of MEK1 E102-I103deletionDepositorInsertMAP2K1 (MAP2K1 Human)
UseLentiviralTagsV5ExpressionMutationE102-I103deletionPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
LEGO-AP1x6-GM55
Plasmid#217415PurposeLuciferase expression vector with 6 AP-1 sites and the minimal GM-CSF promoter. Alias: LEGO-GM55-AP-1x6DepositorInsertLuciferase, GFP
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoter6 AP-1 sites, Human -55 to +28 minimal CSF2 promo…Available sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
LEGO-AP1x3-GM55
Plasmid#217414PurposeLuciferase expression vector with 3 AP-1 sites and the minimal GM-CSF promoter. Alias: LEGO-GM55-AP-1x3DepositorInsertLuciferase, GFP
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoter3 AP-1 sites, Human -55 to +28 minimal CSF2 promo…Available sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
LEGO-pDHS-AP1x6-DUSP5
Plasmid#217418PurposeLuciferase expression vector with 6 AP-1 sites and the full length DUSP5 promoter, and a chromatin priming element. Alias: LEGO-DUSP5p-AP-1x6DepositorInsertLuciferase, GFP
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterHuman DUSP5 promoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV-U6gRNA(BbsI)-PGKpuro2ABFP
Plasmid#50946PurposeAn empty gRNA expression vectorDepositorInsertNone
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCW-Cas9-Blast
Plasmid#83481PurposeLentiviral vector for mammalian expression of doxycycline-inducible Cas9 and constitutive expression of Blasticidin S deaminaase -T2A -rtTADepositorInsertsSpCas9
Blasticidin S deaminase -T2A - reverse tetracycline-controlled transactivator
UseCRISPR and LentiviralTagsExpressionMammalianMutationReplaced puromycin N-acetyltransferase on the ori…PromoterTight Tre and human phosphoglycerate kinase 1 pro…Available sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR_EF1a-->HA-mtLaG2
Plasmid#162225PurposeLentiviral vector for the expression of HA GFP nanobody Lag2 in mammalian cellsDepositorInsertanti-GFP nanobody LaG2
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
FUW-Luc-mCherry-puro
Plasmid#183685PurposeLentiviral vector expressing luciferase and mCherry.DepositorInsertLuc-E2A-mCherry-T2A-PuroR
UseLentiviralTagsExpressionMutationPromoterHuman Ubiquitin C (UbC) promoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR_Ef1a-->flagLaM4_Notch_tTA
Plasmid#162237PurposeLentiviral vector for the expression of LaM 4 mCherry nanobody notch tTA: flagLaM4_Notch_tTA in mammalian cellsDepositorInsertLaM4 mCherry nanobody Notch tTA
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFUGW NLS-Cre
Plasmid#183425PurposeLentiviral Cre vectorDepositorInsertNLS-Cre
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only