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Plasmid#194072Purpose3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blastiDepositorTypeEmpty backboneUseLentiviralTagsV5-TurboID-FLAGExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only
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3XFlagNICD1
Plasmid#20183DepositorInsertNotch 1 Intracellular Domain (Notch1 Mouse)
UseTags3XFLAGExpressionMammalianMutationContains murine Notch 1 Intracellular Domain (Val…PromoterCMVAvailable sinceDec. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
3XFlagNICD2
Plasmid#20184DepositorInsertNotch 2 intracellular domain (Notch2 Mouse)
UseTags3X FLAGExpressionMammalianMutationContains murine Notch 2 Intracellular Domain (Met…PromoterCMVAvailable sinceJan. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1_V5_TurboID_FLAG_Ct
Plasmid#194073Purpose3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blastiDepositorTypeEmpty backboneUseLentiviralTagsFLAG-TurboID-V5ExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_Puro_PGK1_3xFLAG_Twin_Strep
Plasmid#68375PurposeDoyon lab Tandem-Affinity Purification Following (i) Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus and (ii) Endogenous TAP Tagging.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR and TALEN; ZfnTags3xFLAG_Twin_StrepExpressionMammalianMutationPromoterhuman PGK1Available sinceOct. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_Puro_Tet3G_3xFLAG_Twin_Strep
Plasmid#92099PurposeDoyon lab Tandem-Affinity Purification Following Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus. Transgene expression is controlled by an Auto-Regulated Tet-On 3G System.DepositorTypeEmpty backboneUseCRISPR and TALEN; ZfnTagsExpressionMammalianMutationPromoterTet-On 3G BidirectionalAvailable sinceJune 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER20_3xFlag
Plasmid#190028PurposeDoxycycline-inducible lentiviral vector for expression of ORF with C-terminal 3xFlagDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti_EF1a_Flag_EGFP
Plasmid#183117PurposeExpresses flag-tagged EGFP in mammalian cellsDepositorInsertEGFP
UseLentiviralTagsFlagExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEZYflag
Plasmid#18700PurposepFlag-CMV-2 converted Gateway Destination vector for expressing Flag tagged fusion proteins in mammalian cellsDepositorTypeEmpty backboneUseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 26, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDEST_CMV_N_Flag_BIRC6
Plasmid#197973PurposeMammalian cell expression of wt FLAG-BIRC6DepositorInsertBIRC6 (BIRC6 Human)
UseTagsFLAGExpressionMammalianMutationL1332VPromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
Flag-M4-AR
Plasmid#171240PurposeMammalian expression of 3xFlag-tagged human androgen receptor (hAR)DepositorInsert3xFlag-tagged human androgen receptor (AR Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUB_smFLAG_KDM5B_MS2
Plasmid#81084PurposeTranslation reporter: Encodes KDM5B (1544 aa) fused to smFLAG (Spaghetti Monster FLAG: 10 copies of an epitope tag FLAG).DepositorInsertsmFLAG_KDM5B_24xMS2
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFRT_TO_FlagHA_G3BP1
Plasmid#127106DepositorInsertG3BP1 (G3BP1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFLAG_OriM
Plasmid#110097PurposeAnalogous to pFLAG_attP, but containing oriM instead of attP, thus being a replicative vector.DepositorTypeEmpty backboneUseTags3xFLAGExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHIP_RNA2FLAG
Plasmid#61261PurposeFlag tagged Orsay virus RNA2 genome segment expression plasmidDepositorInsertOrsay RNA2
UseTagsFlag fusedExpressionWormMutationPromoterAvailable sinceJan. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV3flag8SHARPIN
Plasmid#50014PurposeMammalian expression of human SHARPIN with a c-terminal 3xFLAG tag, and hygromycin selection.DepositorInsertSHANK-associated RH domain interactor (SHARPIN Human)
UseTags3 x FLAGExpressionMammalianMutationPromoterCMVAvailable sinceDec. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV3flag8HOIL-1L
Plasmid#50016PurposeMammalian expression of human HOIL-1L (RBCK1) with a c-terminal 3xFLAG tag, and hygromycin selection.DepositorInsertRanBP-type and C3HC4-type zinc finger containing 1 HOIL-1L (RBCK1 Human)
UseTags3 x FLAGExpressionMammalianMutationPromoterCMVAvailable sinceDec. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
Occludin_Flag
Plasmid#86042PurposeFull length human occludinDepositorInsertOccludin (OCLN Human)
UseTagsFLAGExpressionMammalianMutationI52T and Q365PPromoterCMVAvailable sinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFastBacMam4_N_Flag_His8_eGFP_TEV
Plasmid#178808PurposeBaculovirus gene transfer into Mammalian cells to product recombinant proteins.DepositorTypeEmpty backboneUseTagsFlag, His8, eGFP, TEVExpressionMammalianMutationPromoterCMVAvailable sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti_EF1a_Flag_TP53_WT
Plasmid#183112PurposeExpresses flag-tagged p53 in mammalian cellsDepositorInsertTP53 (TP53 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationSee Depositor Comments BelowPromoterEF1aAvailable sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only