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pDEST mfap4:tdTomato

Plasmid

#135214

Purpose

Red tagged macrophages

Depositor

David Tobin

Article

Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

Insert

mfap4 promoter and tdTomato

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Available since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pTrex-Neo-tdTomato

Plasmid

#47975

Purpose

Trasgenically expresses tandem tdtomato fluorescent protein in T. cruzi

Depositor

Rick Tarleton

Article

Canavaci et al PLoS Negl Trop Dis. 2010 Jul 13;4(7):e74

Insert

tdTomato

Use

Trypanasoma cruzi expression

Tags

Expression

Mutation

Promoter

T. cruzi rRNA

Available since

April 10, 2015

Availability

Academic Institutions and Nonprofits only

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p1303 DV-SFTPC-tdTomato

Plasmid

#126696

Purpose

donor vector for targeting a tdTomato reporter to the human SFTPC locus at the endogenous ATG start site

Depositor

Darrell Kotton

Article

Jacob et al Cell Stem Cell. 2017 Oct 5;21(4):472-488

Insert

tdTomato

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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SOX17-tdTomato-EhB

Plasmid

#210467

Purpose

Donor plasmid for knock-in tdTomato into the human SOX17 locus

Depositor

Kai Wang

Insert

SOX17 homologous recombination arms with a NLS-tdTomato and EF1alpha-drived hygromycin-NLS-mTagBFP2 selection cassette (SOX17 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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tdTomato-Alpha-Actinin-19

Plasmid

#58070

Purpose

Localization: Alpha Actinin, Excitation: 554, Emission: 581

Depositor

Michael Davidson

Insert

Alpha-Actinin (ACTN1 Human)

Use

Tags

tdTomato

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only
pMEH275 NLS dTomato

Plasmid

#168210

Purpose

Mammalian expression of nuclear-localized dTomato

Depositor

Joshua Leonard

Article

Hung et al J Extracell Vesicles. 2016 May 13;5:3102

Insert

NLS dTomato

Use

Lentiviral

Tags

NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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DD-DamV133A-tdTomato-LMNB1

Plasmid

#159604

Purpose

Shield-1 inducible transient mammalian expression of Dam-tdTomato-LMNB1 for DamID, containing the V133A mutant of Dam and a fused tdTomato to monitor fusion protein localization and expression levels.

Depositor

Aaron Streets

Article

Altemose et al Cell Syst. 2020 Oct 21;11(4):354-366.e9.

Insert

DD-DamV133A-tdTomato-LMNB1

Use

Tags

Expression

Mammalian

Mutation

Dam V133A mutation

Promoter

CMV

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pACAGW-oChiEFtdTomato-AAV

Plasmid

#32846

Purpose

AAV expression of mammalian codon optimized ChIEF for optogenetic excitation.

Depositor

Rory McQuiston

Article

Jurgens et al PLoS One. 2012;7(9):e45717. doi: 10.1371

Insert

oChiEF

Use

AAV

Tags

tdTomato

Expression

Mutation

Promoter

CAGG

Available since

March 19, 2012

Availability

Academic Institutions and Nonprofits only

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pT2Aneo-tdTomato-CAAX

Plasmid

#170284

Purpose

Encoding membrane-anchored tdTomato.

Depositor

Michiyuki Matsuda

Article

Ichise et al Elife. 2022 Feb 3;11. pii: 76269. doi: 1

Insert

tdTomato-CAAX

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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NLS-HA-tdMCP-tdTomato

Plasmid

#183938

Purpose

tandem MS2 binding protein labeled with tdTomato, containing a NLS and a HA-tag

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

tdMCP-tdTomato (cp Synthetic)

Use

Tags

NLS-HA

Expression

Mammalian

Mutation

none

Promoter

CMV (TATA box removed)

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pUAST tdTomato Profilin WT

Plasmid

#53509

Depositor

Matthew Scott

Insert

profilin (chic Fly)

Use

Tags

tdtomato

Expression

Insect

Mutation

Promoter

GAL4

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only
pL0M-S-tdTomato-EC18158

Plasmid

#137078

Purpose

Golden Gate Level 0 S module for N-terminal fluorescent protein tagging

Depositor

Julian Hibberd

Article

Luginbuehl et al Plant Direct. 2020 Feb 11;4(2):e00188. d

Insert

tdTomato DNA synthesis product with 5' and 3' extensions for Golden Gate cloning

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 12, 2020

Availability

Academic Institutions and Nonprofits only

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mT2A-tdTomato-FNF

Plasmid

#182340

Purpose

Recombineering cassette plasmid for T2A-tdTomato-FNF

Depositor

Mario Capecchi

Insert

T2A-tdTomato-FNF

Use

Mouse Targeting; Flp / frt

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-tdTomato

Plasmid

#179794

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Keith Gull, Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

tdTomato

Use

Unspecified

Tags

Expression

Mutation

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pRosa26-4xInsulator (JP6)

Plasmid

#99627

Purpose

IfgMosaic acceptor vector for Classical or Cas9 Gene targeting of the Rosa26 locus

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

Gt(Rosa)26Sor (Gt(ROSA)26Sor Mouse)

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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G1333 DddAtox-N–dSpCas9

Plasmid

#157833

Purpose

expresses split DddAtox-Cas9 construct in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

bpNLS–G1333 DddAtox-N–dSpCas9–UGI–UGI–bpNLS

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-MBP-yATOM_mCherry

Plasmid

#209707

Purpose

Mammalian expression of y-ATOM biosensor for mCherry

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

MBP-yATOM_mCherry

Use

Tags

MBP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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tdTomato-Gamma-Tubulin-17

Plasmid

#58099

Purpose

Localization: Centrosomes, Excitation: 554, Emission: 581

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Gamma-Tubulin (TUBG1 Human)

Use

Tags

tdTomato

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only
CMV-2xFLAG-Atoh1

Plasmid

#101734

Purpose

Expresses mouse Atoh1 with FLAG tag

Depositor

Albert Edge

Article

Parker et al Hum Gene Ther Methods. 2014 Feb;25(1):1-

Insert

Atoh1 (Atoh1 Mouse)

Use

Tags

2x FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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HES7-NLuc-2A-tdTomato

Plasmid

#130932

Purpose

Donor vector for tagging human HES7 C-terminus with NanoLuc and tdTomato

Depositor

James Thomson

Article

Chu et al Cell Rep. 2019 Aug 27;28(9):2247-2255.e5

Insert

HES7 (HES7 Human)

Use

Tags

NanoLuc and tdTomato

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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