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Plasmid#56640PurposeLocalization: Alpha Actinin, Excitation: 530, Emission: 541DepositorInsertAlpha-Actinin (ACTN1 Human)
UseTagsmPapayaExpressionMammalianMutationPromoterCMVAvailable sinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
mKOet-Alpha-Actinin-19
Plasmid#57909PurposeLocalization: Alpha Actinin, Excitation: 548, Emission: 561DepositorInsertAlpha-Actinin (ACTN1 Human)
UseTagsmKOetExpressionMammalianMutationPromoterCMVAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
mKO-Alpha-Actinin-19
Plasmid#57831DepositorInsertAlpha-Actinin (ACTN1 Human)
UseTagsmKOExpressionMammalianMutationPromoterCMVAvailable sinceOct. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
RHPMP132 (mEOS3.1-beta Actin)
Plasmid#88836PurposeLocalization by Super-Resolution microscopy of beta-Actin fused to mEOS3.1DepositorInsertbeta-Actin (ACTB Human)
UseTagsmEOS3.1ExpressionMammalianMutationPromoterCMVAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
RHPMP133 (mOrange2-beta Actin)
Plasmid#88837PurposeLocalization by Super-Resolution microscopy of beta-Actin fused to mOrange2DepositorInsertbeta-Actin (ACTB Human)
UseTagsmOrange2ExpressionMammalianMutationPromoterCMVAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-HDR-mEGFP-Actin
Plasmid#119870PurposeAAV vector including gRNA expression cassette and mEGFP knock-in donor targeting mouse Beta ActinDepositorInsertbeta Actin (Actb Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
Tol2-BetaActin-SecA5-YFP
Plasmid#105664PurposeUbiquitously expresses a secreted and fluorescently tagged Annexin5 for in-vivo apoptosis reporterDepositorInsertSecreted Annexin5-EYFP (ANXA5 Human)
UseTol2-gatewayTagsEYFPExpressionMutationPromoterBeta-ActinAvailable sinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGTag-gal4-VP16-B-actin
Plasmid#117817PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertgal4/VP16
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
YFP NLS Beta-Actin
Plasmid#60613PurposeEncodes YFP and NLS tagged beta-actinDepositorInsertBeta-actin (ACTB Human)
UseTagsEYFP and NLSExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
α-actinin-4-M522-Venus in pcDNA6
Plasmid#190802PurposeImaging of α-actinin-4 in live cellsDepositorInsertalpha-actiinin-4-M522-venus (ACTN4 Human, Synthetic)
UseTagsHis, myc, and venusExpressionMammalianMutationPromoterCMVAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBactin-Nedd1-mOrange2-hCM1
Plasmid#196863PurposeExpression of neural precursor cell expressed developmentally down-regulated 1 (Nedd1) fused to mOrange2 and human (h) Centrosomin motif 1 (CM1). Nedd1 targets CM1 to the centrosome to activate gamma-TuRCDepositorInsertNedd1-mOrange2-hCM1 (Nedd1 Human, Rat)
UseTagsExpressionMammalianMutationPromoterChicken bactin (plus Chicken bactin intron)Available sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR-NLS-actin-R62D
Plasmid#118381PurposeGateway compatible donor vector with nuclear localization signal (NLS) attached to actin-R62D.DepositorInsertactin beta (ACTB Human)
UseDonor-plasmidTagsExpressionMutationNLS and R62DPromoterAvailable sinceNov. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBactin-td-mCherry-Cdk5rap2-CTD
Plasmid#196854PurposeExpression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to tandem (td)-mCherry. Used to displace endogenous Cdk5rap2 from centrosomes.DepositorInserttd-mCherry-Cdk5rap2-CTD (Cdk5rap2 Discosoma sp.)
UseTagsExpressionMammalianMutationNone (wt)PromoterChicken bactin (plus Chicken bactin intron)Available sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBactin-mOrange2-NEDD1-gTBD
Plasmid#196858PurposeExpression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange. Used to displace endogenous gamma-TuRC from the centrosome.DepositorInsertmOrange2-N-gTBD (NEDD1 Human)
UseTagsExpressionMammalianMutationPromoterChicken bactin (plus Chicken bactin intron)Available sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKanCMV-mClover3-18aa-actin
Plasmid#74259PurposeMammalian expression of N-terminal fusion of mClover3 to actinDepositorInsertActin
UseTagsmClover3ExpressionMammalianMutationPromoterCAG and CMVAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKanCMV-mRuby3-18aa-actin
Plasmid#74255PurposeMammalian expression of N-terminal fusion of mRuby3 to actinDepositorInsertactin
UseTagsmRuby3ExpressionMammalianMutationPromoterCAG and CMVAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
ACTIN-SOX2-PGK Cre
Plasmid#59019PurposeBicistronic Lentivirus expressing sox2 and CreDepositorInsertSox2 (Sox2 Mouse)
UseLentiviralTagsExpressionMutationPromoterB-actinAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBactin-AcGFP-Cdk5rap2-CTD
Plasmid#196853PurposeExpression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to AcGFP. Used to displace endogenous Cdk5rap2 from centrosomes.DepositorInsertAcGFP-Cdk5rap2-CTD (Cdk5rap2 Rat)
UseTagsExpressionMammalianMutationNone (wt)PromoterChicken bactin (plus Chicken bactin intron)Available sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBactin-EGFP-NEDD1-gTBD
Plasmid#196857PurposeExpression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to enhanced (E) GFP. Used to displace endogenous gamma-TuRC from the centrosome.DepositorInsertEGFP-N-gTBD (NEDD1 Human)
UseTagsExpressionMammalianMutationPromoterChicken bactin (plus Chicken bactin intron)Available sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pActin5c-CD-dCas9m4-UDI-attB
Plasmid#104879PurposeTo generate point mutations using CRISPR/Cas9 system; encoded cytidine deaminase fused to deactived Cas9DepositorInsertCD-dCas9(m4)-UDI
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only