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pBBK24 pCAS-Tyr-[gRNA: 7=HIS3] (SplitHygR, AmpR)

Plasmid

#179008

Purpose

Sp.Cas9 and gRNA yeast expression vector with HIS3 gRNA pre-cloned. Selection =SplitHygromycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK18 pCAS-Tyr-[gRNA: 1=FF18] (SplitHygR, AmpR)

Plasmid

#179002

Purpose

Sp.Cas9 and gRNA yeast expression vector with FF18 gRNA pre-cloned. Selection =SplitHygromycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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PiggyBac dCas9-KRAB

Plasmid

#191314

Purpose

piggyBac integration and dox-inducible expression of KRAB-dCas9-HA-tagBFP

Depositor

Joanna Wysocka

Article

Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

Inserts

KRAB-dCas9-HA-tagBFP
rtTA3-IRES-PURO

Use

CRISPR

Tags

HA and tagBFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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PiggyBac dCas9-VPR

Plasmid

#191269

Purpose

piggyBac integration and dox-inducible expression of VPR-dCas9-HA-tagBFP

Depositor

Joanna Wysocka

Article

Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

Inserts

VPR-dCas9-HA-tagBFP
rtTA3-IRES-PURO

Use

CRISPR

Tags

HA and tagBFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pMJ806

Plasmid

#39312

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 7, 2012

Availability

Academic Institutions and Nonprofits only

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Cas9 TLS2

Plasmid

#196978

Purpose

Expresses Cas9 fused with TLS2 mobility signal that confers graft mobility. Induced by estradiol.

Depositor

Friedrich Kragler

Article

Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

Cas9 with TLS2 tag

Use

Tags

Expression

Plant

Mutation

Promoter

pG10-90

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-dCas9-Dnmt3a-Dnmt3l-P2A-eGFP

Plasmid

#128424

Purpose

Human Codon Optimized dCas9-DNMT3A3L

Depositor

Bradley Bernstein

Article

Tarjan et al Nat Commun. 2019 Sep 18;10(1):4258. doi:

Insert

dCas9m4-sv40NLS-DNMT3A-3L-sv40NLS-P2A-eGFP

Use

CRISPR and Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

T7 Promoter

Available since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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Cas9 TLS1

Plasmid

#196977

Purpose

Expresses Cas9 fused with TLS1 mobility signal that confers graft mobility. Induced by estradiol.

Depositor

Friedrich Kragler

Article

Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

Cas9 with TLS1 tag

Use

Tags

Expression

Plant

Mutation

Promoter

pG10-90

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pDN-Sth1Cas9

Plasmid

#221386

Purpose

Cas9 under control of AHT inducible TetR on integrating plasmid (KanR) using L5 integrase and attP for site-specific integration into attB

Depositor

Deborah Hung

Article

Neo et al J Bacteriol. 2024 Mar 21;206(3):e0033523

Insert

Sth1 Cas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pMP11

Plasmid

#215559

Purpose

pKD46 with constitutively expressed Cas9 and an aTc gRNA targeting the ColE1 origin

Depositor

Brian Pfleger

Article

Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

Inserts

Cas9
gRNA targeting pBR322 origin of replication

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23105 and tet

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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AW01_pU.CAG.eSpCas9(1.1).rBGpA

Plasmid

#199255

Purpose

Expression construct encoding the high specificity eSpCas9(1.1) nuclease

Depositor

Manuel Goncalves

Article

Chen et al Nucleic Acids Res. 2016 Jun 8. pii: gkw5

Insert

High specificity eSpCas9(1.1) nuclease

Use

CRISPR

Tags

3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Mutation

K848A K1003A R1060A

Promoter

CAG promoter

Available since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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Cas9 Null

Plasmid

#198727

Purpose

Expresses Cas9 without a mobility signal and the mRNA is not graft mobile. Induced by estradiol.

Depositor

Friedrich Kragler

Article

Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

Cas9

Use

Tags

Expression

Plant

Mutation

Promoter

pG10-90

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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EC64422

Plasmid

#191768

Purpose

CRISPR mutagenesis in Medicago truncatula

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:221-252. doi

Insert

SpCas9

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pRU295

Plasmid

#167694

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pEC1.2::Cas9i

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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MLM3613

Plasmid

#42251

Purpose

Expresses Cas9 nuclease (Streptococcus pyogenes) from CMV and T7 promoters

Depositor

Keith Joung

Article

Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

Insert

Streptococcus pyogenes Cas9

Use

CRISPR; Zebrafish expression

Tags

Expression

Mutation

Promoter

CMV

Available since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only

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pRU294

Plasmid

#167689

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pEC1.2::Cas9i

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pRU324

Plasmid

#167691

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pEC1.2::Cas9i

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pScI_dCas9-CDA-UL

Plasmid

#108551

Purpose

Bacterial Target-AID UGI-LVA vector

Depositor

Akihiko Kondo

Article

Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

Insert

dCas9-PmCDA1-UGI-LVA

Use

Tags

Flag

Expression

Bacterial

Mutation

D10A and H840A for SpCas9

Promoter

lambda OR

Available since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pMJ826

Plasmid

#39316

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Mutation

Histidine 840 to Alanine (H840A)

Promoter

T7

Available since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only

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pMOD_A-CmYLCV-dCas9-24XGP41

Plasmid

#202009

Purpose

Moclo level 1 - Module A, Promoter: CmYLCV, Gene: dCas9-24XGP41, Terminator: HSP

Depositor

Michael Smanski

Article

Casas-Mollano et al Nucleic Acids Research, Volume 51, Issue

Insert

dCas9-24XGP41

Use

Synthetic Biology; Moclo level 1 vector

Tags

GS linkers

Expression

Mutation

Promoter

CmYLCV

Available since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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