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Plasmid#204239PurposeBacterial expression of A. arcticum malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDH_AQUAR
UseTags6X HisExpressionBacterialMutationPromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET24a MDH_9CREN
Plasmid#204243PurposeBacterial expression of I. islandicus malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDH_9CREN
UseTags6X HisExpressionBacterialMutationPromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a MDHC_YEAST
Plasmid#204232PurposeBacterial expression of yeast cytosolic malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDHC_YEAST (MDH2 Budding Yeast)
UseTags6X HisExpressionBacterialMutationPromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-FIP3
Plasmid#74753PurposeExpressed sfGFP-tagged FIP3 in E. coli for protein productionDepositorInsertRab11 family interacting protein 3 (RAB11FIP3 Human)
UseTags6x His, Strep II, and superfolder GFPExpressionBacterialMutationPromoterT7Available sinceJune 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET21a.Tat(48-57)-EGFP
Plasmid#165040PurposeExpression of Tat(48-57) conjugated EGFP for protein purification (6XHis tag)DepositorInsertEGFP
UseTags6XHis and Tat(48-57)ExpressionBacterialMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-AncMEcoGII291
Plasmid#205024PurposeExpressing engineered M.EcoGII with higher thermostability and DNA methylation activityDepositorInsertAncestral M.EcoGII 291
UseTagsHis6x tagExpressionBacterialMutationPromoterT7Available sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SpyTagMBP
Plasmid#35050DepositorInsertSpyTagMBP
UseTagsHis6ExpressionBacterialMutationSimple peptide tag added to the N-terminus of MBP…PromoterT7 promoterAvailable sinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28-H12TEV
Plasmid#208635Purposefor E. coli expression of large protein, insert 6xhis + TEV site using NdeI and BamHI site in pET28a(+), note that it is 6xHis-Thrombin-6xHis-TEV, not continuous 12xHis. BamHI in-frame with tag.DepositorTypeEmpty backboneUseTags6xhisExpressionBacterialMutationPromoterT7Available sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SnoopCatcher
Plasmid#72322PurposeBacterial expression of SnoopCatcher, an engineered protein forming a spontaneous covalent bond with its cognate peptide partner SnoopTag.DepositorInsertpET28a-SnoopCatcher
UseTagsHis6 tagExpressionBacterialMutationchanged G842T and D848G of S. pneumoniae adhesin …PromoterT7Available sinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Ec.coaA
Plasmid#50386PurposeHeterologous expression of E. coli CoA biosynthetic enzyme, PanK (pantothenate kinase), induced with IPTG. Contain 6xHis-tag for nickel affinity purification.DepositorInsertPanK
UseTags6XHis-tagExpressionBacterialMutationPromoterT7Available sinceMarch 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MSP1D1deltaH4H5
Plasmid#71716PurposeMembrane scaffold protein (MSP), helix 4+ helix 5 deletionDepositorInsertApolipoprotein A1 (APOA1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
Nampt-PET28a
Plasmid#25630DepositorInsertNampt (Nampt Mouse)
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceAug. 9, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SpyCatcher
Plasmid#223633PurposeProtein purification of SpyCatcher003 (S49C) with 6xHis tag from E. coliDepositorInsertSpyCatcher003 S49C
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)- PC-MjMAT
Plasmid#179372PurposePC-MjMAT is an engineered methionine adenosyltransferase that catalyses the chemo-enzymatic generation of AdoMet analogs carrying photocaging groups.DepositorInsertPC-MjMAT
UseTags6xHisExpressionBacterialMutationL147A, I351APromoterT7Available sinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET24a-ncOGT-FL
Plasmid#190821PurposeFor expression of full-length human ncOGT in E.coli. The ncOGT is 6x His-tagged on its N-terminus and codon-optimized for E.coli.DepositorInsertO-GlcNAc Transferase (OGT Human)
UseTags6x HisExpressionBacterialMutationCodon optimized for expression in E.coliPromoterT7Available sinceJan. 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a-AncMEcoGII250
Plasmid#205025PurposeExpressing engineered M.EcoGII with higher thermostabilityDepositorInsertAncestral M.EcoGII 250
UseTagsHis6x tagExpressionBacterialMutationPromoterT7Available sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_Caspase-4_K356D
Plasmid#214312PurposeProtein overexpression in bacteriaDepositorInsertCASP4 (CASP4 Human)
UseTagsHis-TEVExpressionBacterialMutationK356DPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET26b-Granzyme A
Plasmid#8823DepositorInsertHuman Granzyme A (GZMA Human)
UseTags(His)6 and Enterokinase siteExpressionBacterialMutationit mature enzyme.PromoterAvailable sinceSept. 26, 2005AvailabilityAcademic Institutions and Nonprofits only -
pET29b-pduCDE
Plasmid#110138PurposeExpresses B12-dependent propanediol dehydratase in E. coli BL21(DE3)DepositorUseTagsExpressionBacterialMutationPromoterT7Available sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
nifF in pET21b
Plasmid#124520PurposeAzotobacter vinelandii flavodoxin II of NitrogenaseDepositorInsertFlavodoxin II (nifF Azotobacter vinelandii)
UseTagsNoneExpressionBacterialMutationPromoterT7 / lacAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only