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pCALNL-eGFP-Esp3I

Plasmid

#81226

Purpose

Cloning plasmid derived from loxP reporter. The Kan and pA where digested and removed with XhoI/MluI and replaced with two Esp3I sites

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

Esp31-Neo-Esp31 deletion cassette upstream of EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCBa

Available since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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HS-Utrophin-GFP

Plasmid

#105952

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

Utrophin (Utrn Mouse)

Use

Tags

GFP

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-PASS

Plasmid

#193970

Purpose

Monitoring the production of phosphatidic acid with GFP-PASS (phosphatidic acid biosensor with superior sensitivity)

Depositor

Guangwei Du

Article

Zhang et al Mol Cell Biol. 2014 Jan;34(1):84-95. doi

Insert

PA biosenor

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pLentiGuide_GFP_sgEPAS1#2

Plasmid

#183120

Purpose

pLentiGuide modified to express GFP and an sgRNA targeting EPAS1

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

EPAS1-2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR

Plasmid

#60231

Purpose

Expresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

Depositor

Feng Zhang

Article

Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

Inserts

sgRNA
Cre recombinase
EGFP

Use

AAV, CRISPR, Cre/Lox, and Mouse Targeting

Tags

Cre-HA-P2A and EGFP-KASH

Expression

Mammalian

Mutation

Promoter

U6 and hSyn

Available since

Oct. 9, 2014

Availability

Academic Institutions and Nonprofits only

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AAV:ITR-U6-sgRNA(NeuN)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR

Plasmid

#60230

Purpose

Expresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

Depositor

Feng Zhang

Article

Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

Inserts

sgRNA
Cre recombinase
EGFP

Use

AAV, CRISPR, Cre/Lox, and Mouse Targeting

Tags

Cre-HA-P2A and EGFP-KASH

Expression

Mammalian

Mutation

Promoter

U6 and hSyn

Available since

Nov. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pFOX13-Tier1-PhCMV-tGFP

Plasmid

#169510

Purpose

Tier-1 vector encoding PhCMV-driven green fluorescent protein turbo GFP (PhCMV-tGFP-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven turbo green fluorescent protein

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pFOX12-Tier1-PhCMV-eGFP

Plasmid

#169509

Purpose

Tier-1 vector encoding PhCMV-driven green fluorescent protein eGFP (PhCMV-eGFP-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven enhanced green fluorescent protein

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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ath5:GFP-DCX

Plasmid

#105959

Purpose

transgenesis, doublecortin DCX

Depositor

Caren Norden

Article

Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

Insert

DCX (DCX Human)

Use

Tags

GFP

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pmEGFP-C1-Nir3-LNS2

Plasmid

#220087

Purpose

C-terminal PA binding domain of Nir3

Depositor

Gerry Hammond

Article

Weckerly et al bioRxiv [Preprint]. 2024 Feb 28:2024.02.

Insert

PITPNM2 (PITPNM2 Human)

Use

Tags

mEGFP-GGGGSHM

Expression

Mammalian

Mutation

amino acids 925-1209

Promoter

CMV

Available since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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Vmn2r1-D-tauGFP TV

Plasmid

#108080

Purpose

Targeting vector for Vmn2r1 gene knockout mice (∆C1-GFP strain)

Depositor

Peter Mombaerts

Article

Akiyoshi et al Eur J Neurosci. 2018 Apr;47(7):887-900.

Insert

Vmn2r1-D-tauGFP-pA-ACNF (Vmn2r1 Mouse)

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

May 17, 2019

Availability

Academic Institutions and Nonprofits only

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pMD1 anti-GFP nanobody-Tn5

Plasmid

#215567

Purpose

Expresses anti-GFP nanobody-Tn5 fusion in bacteria with an N-terminal 3xFlag tag and a C-terminal intein-CBD-intein tag

Depositor

Isabelle Mansuy

Article

Maria A. et al bioRxiv 2024.07.12.603224

Insert

anti-GFP nanobody-Tn5 fusion

Use

Tags

3xFlag and intein-CBD

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CBh-DIO-CB2-EGFP

Plasmid

#91946

Purpose

Expression of mouse CB2 cannabinoid receptors and eGFP in the presence of Cre

Depositor

Jimok Kim

Article

Li et al Neuroscience. 2017 Sep 6. pii: S0306-452

Inserts

EGFP
type-2 cannabinoid receptor

Use

AAV

Tags

FLAG and None

Expression

Mutation

Promoter

Available since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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AiP5002 RabV-SADdeltaG-histone-EGFP

Plasmid

#176284

Purpose

Recombinant rabies virus expressing histone-EGFP

Depositor

The Allen Institute for Brain Science, Shenqin Yao

Article

Yao et al Nat Neurosci. 2023 Feb;26(2):350-364. do

Insert

H2B (H2BC11 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pAS_4xMma PylT_GFP 150TAG 6xHis

Plasmid

#140015

Purpose

Plasmid with 4xMmaPylT cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

sfGFP

Use

Tags

6xHis

Expression

Mammalian

Mutation

150TAG

Promoter

EF1

Available since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pAS_4xG1 PylT sfGFP 150TAG

Plasmid

#154767

Purpose

plasmid with 4xG1 PylT cassette and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

sfGFP

Use

Tags

Expression

Mammalian

Mutation

150TAG

Promoter

EF1

Available since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pAS_4xMx PylT_GFP 150TAG

Plasmid

#140016

Purpose

Plasmid with 4xMxPylT cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

sfGFP

Use

Tags

Expression

Mammalian

Mutation

150TAG, K46E (Please see depositor comments below)

Promoter

EF1

Available since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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HLH Bmal Pas 1-465-VenC F423R/V435R

Plasmid

#47371

Purpose

Bmal fragment cloned with Venus tag used for mutation

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Use

Tags

Venus aa 156-239

Expression

Mammalian

Mutation

aa 1-465, F423R/V435R

Promoter

CMV

Available since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pAS_4xMx PylT C41CA_GFP 150TAG

Plasmid

#140017

Purpose

Plasmid with 4xMxPylT C41CA cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

sfGFP

Use

Tags

Expression

Mammalian

Mutation

150TAG

Promoter

EF1

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pAS_4xhyb PylT sfGFP 150TAG

Plasmid

#154771

Purpose

plasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid ) and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

sfGFP

Use

Tags

Expression

Mammalian

Mutation

150TAG

Promoter

EF1

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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