Addgene

Showing: 281 - 300 of 542 results

Results per page:

Sort results by:

mCherry-miniSOG-RatLC3

Plasmid

#55095

Purpose

Localization: Microtubule-Binding Protein, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

LC3

Use

Tags

mCherry-miniSOG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
pEX-CFP-hLC3ΔG

Plasmid

#24986

Depositor

Isei Tanida

Article

Tanida et al Autophagy. 2008 Jan 1. 4(1):131-4.

Insert

a mutant human MAP1-LC3B fused with ECFP (MAP1LC3B Human)

Use

Tags

ECFP

Expression

Mammalian

Mutation

deleted Gly at 121

Promoter

Available since

June 21, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223 LC3A G120

Plasmid

#123071

Purpose

Gateway entry clone encoding human MAP1LC3A G120

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)

Use

Gateway entry vector / entry clone

Tags

Expression

Mutation

Deleted amino acid 121. Stop codon after G120.

Promoter

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
[pSK581] pRK5 HA-SLC38A9N

Plasmid

#136141

Purpose

mammalian expression of SLC38A9 N term fragment (1-119)

Depositor

Kuang Shen, David Sabatini

Article

Shen et al Proc Natl Acad Sci U S A. 2018 Sep 18;11

Insert

SLC38A9 N term fragment (1-119) (SLC38A9 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223 LC3B2 G120

Plasmid

#123077

Purpose

Gateway entry clone encoding human MAP1LC3B2 G120

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3 beta 2 (MAP1LC3B2 Human)

Use

Gateway entry vector / entry clone

Tags

Expression

Mutation

Deleted amino acids 121-125. Stop codon after G12…

Promoter

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223 LC3B2 G120A

Plasmid

#123076

Purpose

Gateway entry clone encoding human MAP1LC3B2 G120A

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3 beta 2 (MAP1LC3B2 Human)

Use

Gateway entry vector / entry clone

Tags

Expression

Mutation

Glycine 120 to Alanine

Promoter

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223 LC3B G120

Plasmid

#123074

Purpose

Gateway entry clone encoding human MAP1LC3B G120

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Use

Gateway entry vector / entry clone

Tags

Expression

Mutation

Deleted amino acids 121-125. Stop codon after G12…

Promoter

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
SLC30A10-delta105-107

Plasmid

#65205

Purpose

Constitutive expression of SLC30A10 delta 105-107 in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Article

Leyva-Illades et al J Neurosci. 2014 Oct 15;34(42):14079-95.

Insert

SLC30A10 (SLC30A10 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

delta 105-107 amino acids

Promoter

CMV

Available since

May 19, 2015

Availability

Academic Institutions and Nonprofits only
pMRXIB-mRuby3-LC3

Plasmid

#221738

Purpose

Stable expression of mRuby3-LC3 in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

LC3B (Map1lc3b Rat)

Use

Retroviral

Tags

mRuby3

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
SNAP-LC3 HRD

Plasmid

#207545

Purpose

Homologous recombination donor to integrate a SNAP-Tag at the endogenous LC3 locus in human cells

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

SNAPtag with internal PuroR cassette flanked by human LC3B locus sequences

Use

Tags

SNAPtag

Expression

Mammalian

Mutation

Promoter

Endogenous

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Halo-LC3B HRD

Plasmid

#207539

Purpose

Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous LC3B locus in human cells

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

HaloTag with internal PuroR cassette flanked by human LC3B locus sequences

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

Endogenous

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-Mch-MAP1LC3B

Plasmid

#201924

Purpose

For lentiviral transduction of Mch-MAP1LC3B

Depositor

Wade Harper

Article

Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

Insert

MAP1LC3B (MAP1LC3B Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAsh-MCL-LC3B

Plasmid

#204573

Purpose

Expresses Ash-tagged LC3B in mammalian cells

Depositor

Masaaki Komatsu

Article

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

LC3B (MAP1LC3B Human)

Use

Tags

Expression

Mammalian

Mutation

delta121-125aa

Promoter

Available since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX FLAG-SLC38A9.1

Plasmid

#199749

Purpose

Lentiviral construct to stably express SLC38A91.1

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

SLC38A9.1

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
PGK EGFP-LC3B

Plasmid

#200427

Purpose

Mammalian expression of fluorescently-tagged marker for autophagic vesicles

Depositor

Erika Holzbaur

Article

Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

Insert

MAP1LC3B (MAP1LC3B Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

PGK

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
TalO8-GLC3-CFP

Plasmid

#178307

Purpose

CoTrIP plasmid with GLC3 promoter driving CFP's expression

Depositor

Brian Zid

Article

Chen et al Elife. 2022 Sep 15;11. pii: 76965. doi:

Insert

Glc3pr-CFP-tAdh1

Use

Tags

Expression

Yeast

Mutation

Promoter

GLC3 and NA

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-3×FLAG-SLC35A2

Plasmid

#186283

Purpose

transient expression of N-terminal 3×FLAG tagged SLC35A2 isoform c/UGT1 in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Article

Li et al J Biol Chem. 2021 Sep;297(3):101069. doi

Insert

SLC35A2 (SLC35A2 Human)

Use

Tags

3×FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP.N3-myc-SLC35A2

Plasmid

#186285

Purpose

transient expression of N-terminal myc tagged SLC35A2 isoform c/UGT1 in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Article

Li et al Glycobiology. 2019 Jun 1;29(6):490-503.

Insert

SLC35A2 (SLC35A2 Human)

Use

Tags

MYC

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pStA0::SPLc3-RBSc110

Plasmid

#171893

Purpose

Promoter SPLc3 and RBSc110 in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2021 Dec 2;49(21):e12

Insert

Promoter SPLc3 and RBSc110

Use

Synthetic Biology

Tags

Expression

Mutation

N/A

Promoter

Available since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pStA0::SPLc3-RBSc249

Plasmid

#171903

Purpose

Promoter SPLc3 and RBSc249 in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2021 Dec 2;49(21):e12

Insert

Promoter SPLc3 and RBSc249

Use

Synthetic Biology

Tags

Expression

Mutation

N/A

Promoter

Available since

Feb. 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge