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pInducer-myc-ERK1-R84S/D338N

Plasmid

#213594

Purpose

Doxycycline inducible expression of myc-tagged ERK1-R84S/D338N cDNA in mammilian cells

Depositor

Channing Der

Article

Klomp et al Science. 2024 Jun 7;384(6700):eadk0850.

Insert

MAPK3 (MAPK3 Human)

Use

Lentiviral

Tags

MYC

Expression

Mammalian

Mutation

R84S, D338N

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGP-RSET-iGABASnFR2(no bind)-6xHis

Plasmid

#218887

Purpose

Bacterial expression of improved GABA sensor control (no change in fluorescence)

Depositor

GENIE Project

Article

Kolb et al bioRxiv 2025.03.25.644953

Insert

iGABASnFR2(no bind)

Use

Tags

6xHis

Expression

Bacterial

Mutation

S99A F102G R168P

Promoter

T7

Available since

May 14, 2024

Availability

Academic Institutions and Nonprofits only

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pMV2 AAV-RapaInducible-NFZ-HGF

Plasmid

#188749

Purpose

Rapamycin inducible AAV vector expressing HGF

Depositor

Lacra Bintu, Michael Bassik

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

HGF (HGF Human)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Rapamycin inducible

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pJT314 AAV-Rapamycin Inducible-NZ-Citrine

Plasmid

#202063

Purpose

Inducible AAV activator

Depositor

Lacra Bintu, Michael Bassik

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

AAV-Rapamycin Inducible-NZ-Citrine

Use

AAV; Aav with reporter

Tags

Expression

Mammalian

Mutation

ITR deletion

Promoter

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pJT365 AAV-Rapamycin Inducible-S3H

Plasmid

#202066

Purpose

Inducible AAV activator

Depositor

Lacra Bintu, Michael Bassik

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

AAV-Rapamycin Inducible-S3H

Use

AAV; Aav with reporter

Tags

Expression

Mammalian

Mutation

ITR deletion

Promoter

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pJT366 AAV-Rapamycin Inducible QNZF

Plasmid

#202067

Purpose

Inducible AAV activator

Depositor

Lacra Bintu, Michael Bassik

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

AAV-Rapamycin Inducible QNZF

Use

AAV; Aav with reporter

Tags

Expression

Mammalian

Mutation

ITR deletion

Promoter

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pJT313 AAV-Rapamycin Inducible-ZF-Citrine

Plasmid

#202062

Purpose

Inducible AAV activator

Depositor

Lacra Bintu, Michael Bassik

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

AAV-Rapamycin Inducible-ZF-Citrine

Use

AAV; Aav with reporter

Tags

Expression

Mammalian

Mutation

ITR deletion

Promoter

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-CALB1/calbindin [L109/39R-1]

Plasmid

#206686

Purpose

Mammalian Expression Plasmid of anti-CALB1/calbindin (Human). Derived from hybridoma L109/39-1.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-CALB1/calbindin (Homo sapiens) recombinant Mouse monoclonal antibody (CALB1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-CALB1/calbindin [L109/39R-2b]

Plasmid

#206635

Purpose

Mammalian Expression Plasmid of anti-CALB1/calbindin (Human). Derived from hybridoma L109/39-2b.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-CALB1/calbindin (Homo sapiens) recombinant Mouse monoclonal antibody (CALB1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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Tet-inducible 3xFlag Mouse Caspase-9 ΔB LS

Plasmid

#194927

Purpose

Inducible Expression of N-Terminal 3X Flag Mouse Caspase-9 Minus Large Subunit Region Ala 264 to Asp 353

Depositor

Hannah Rabinowich

Article

Han et al J Biol Chem. 2014 Mar 7;289(10):6485-649

Insert

N-Terminal 3X Flag Mouse Caspase-9 Minus Sequence Encoding Ala 264 to Asp 353 (Casp9 Mouse)

Use

Tags

3X Flag

Expression

Mammalian

Mutation

Deletion of Large Subunit Region Ala 264 to Asp 3…

Promoter

CMV

Available since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Tet-Puro-shMMERVK9c_1 (inducible)

Plasmid

#189957

Purpose

shRNA mediated knockdown

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

ERV_MMERVK9c

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Tet-Puro-shMMERVK10c_2 (inducible)

Plasmid

#189956

Purpose

shRNA mediated knockdown

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

ERV_MMERVK10c

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Tet-Puro-shMMETn_2 (inducible)

Plasmid

#189954

Purpose

shRNA mediated knockdown

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

ERV_MMETn

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Tet-Puro-shMMETn_1 (inducible)

Plasmid

#189953

Purpose

shRNA mediated knockdown

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

ERV_MMETn

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Tet-Puro-shMMERVK9c_2 (inducible)

Plasmid

#189958

Purpose

shRNA mediated knockdown

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

ERV_MMERVK9c

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 FIIND F405G + S297A

Plasmid

#169974

Purpose

pDONR221 for Gateway shuttling. Contains the autoprocessing-deficient CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (F405G)

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway cloning vector

Tags

Expression

Mutation

S297A, F405G

Promoter

Available since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 FIIND E274R + S297A

Plasmid

#169968

Purpose

pDONR221 for Gateway shuttling. Contains the CARD8 autoprocessing-deficient FIIND (L162M-P434, no stop codon) that cannot bind DPP9 at site I (E274R)

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway cloning vector

Tags

Expression

Mutation

S297A, E274R

Promoter

Available since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 FIIND R394E + S297A

Plasmid

#169972

Purpose

pDONR221 for Gateway shuttling. Contains the autoprocessing-deficient CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (R394E)

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway cloning vector

Tags

Expression

Mutation

S297A, R394E

Promoter

Available since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 NLRP1 FIIND (F1079M-L1373)

Plasmid

#166831

Purpose

Gateway pDONR vector containing NLRP1 FIIND (no stop codon)

Depositor

Daniel Bachovchin

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Gateway shuttling vector

Tags

Expression

Mutation

Promoter

Available since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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Designed HEL-binding scFv D44.1des

Plasmid

#111719

Purpose

Yeast Surface Display of D44.1des

Depositor

Sarel Fleishman

Article

Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

Insert

D44.1 des

Use

Tags

cmyc tag

Expression

Yeast

Mutation

L Q89G, L S55V, L S43P, H V37M, H H43G, H W47Y, H…

Promoter

Available since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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