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Plasmid#110165PurposeminiMos vector expresses ERKKTR-mClover and mCherry-H2B in C. elegans VPCs; request with plasmids pCC335 and pCC336 for unphosphorylated and phosphomimetic controls (Contains Neomycin resistance)DepositorInsertERK-KTR-mClover-T2A-mCherry-H2B
UseMinimos transposonTagsmCherry and mCloverExpressionWormMutationR61K change made in ERK-KTR CDSPromoterlin-31 promoter/enhancerAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCC336
Plasmid#110167PurposeminiMos vector expresses ERKKTR(S43E,T55E,T62E)-mClover and mCherry-H2B in C. elegans VPCs; use as control for pCC324 (Contains Neomycin resistance)DepositorInsertERK-KTR(S43E,T55E,T62E)-mClover-T2A-mCherry-H2B
UseMinimos transposonTagsmCherry and mCloverExpressionWormMutationPhospho-acceptor sites mutated: S43E, T55E, T62E.…Promoterlin-31 promoter/enhancerAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCC335
Plasmid#110166PurposeminiMos vector expresses ERKKTR(S43A,T55A,T62A)-mClover and mCherry-H2B in C. elegans VPCs; use as control for pCC324 (Contains Neomycin resistance)DepositorInsertERK-KTR(S43A,T55A,T62A)-mClover-T2A-mCherry-H2B
UseMinimos transposonTagsmCherry and mCloverExpressionWormMutationPhospho-acceptor sites mutated: S43A, T55A, T62A.…Promoterlin-31 promoter/enhancerAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTALYM3B15
Plasmid#47878PurposeTALE-mClover against mouse major satellite sequenceDepositorInsertTALE-mClover
UseTALENTagsmCloverExpressionMammalianMutationPromoterCAGAvailable sinceOct. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTALYM3-Telo1
Plasmid#47883PurposeTALE-mClover against telomere sequenceDepositorInsertTALE-mClover
UseTALENTagsmCloverExpressionMammalianMutationPromoterCAGAvailable sinceOct. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-08
Plasmid#119987PurposeTemplate for C-terminal PCR tagging of mammalian genes (without selection) with mClover3DepositorInsertmClover3
UsePcr templateTagsmClover3ExpressionMutationPromoterNoneAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK265 (RAD21-mAC Hygro)
Plasmid#140539PurposeRAD21 tagging with mAID-CloverDepositorInsertRAD21 (RAD21 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
CTCF-mAC donor (Neo)
Plasmid#140645PurposeCTCF tagging with mAID-CloverDepositorInsertCTCF (CTCF Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
CTCF-mAC donor (Hygro)
Plasmid#140646PurposeCTCF tagging with mAID-cloverDepositorInsertCTCF (CTCF Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMK262 (RAD21-mAC Neo)
Plasmid#140538PurposeRAD21 tagging with mAID-CloverDepositorInsertRAD21 (RAD21 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE3_ mTFP1- Vamp2
Plasmid#201214PurposeAAV expression of TRE(TRE3G)-driven, Vamp2-conjugated mTFP1 expression for fluorescent labling of axon terminalsDepositorInsertmTFP1 (from addgene #54613)
UseAAVTagsmouse Vamp2ExpressionBacterial and MammalianMutationPromoterTRE3GAvailable sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z08
Plasmid#120051PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mClover3DepositorInsertmClover3
UsePcr templateTagsmClover3ExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P08
Plasmid#120019PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mClover3DepositorInsertmClover3
UsePcr templateTagsmClover3ExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM30
Plasmid#135490PurposeAgrobacterium T-DNA with Spun H2Bpr driving hph-mClover3DepositorInserthph SpunH2A/Bpr hph-mClover3
UseTagsmClover3ExpressionBacterial and YeastMutationPromoterSpunH2A/Bpr (divergent)Available sinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMK1253
Plasmid#133058PurposeLentiviral vector with EF1A promoter constitutively expressing a single insert containing the Tau.K18(P301L/V337M)-Clover2 fusion protein.DepositorInsertTau.K18(LM)
UseLentiviralTagsmClover2ExpressionMammalianMutationPromoterEF1AAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
EB3N-VVDfast-mCl3-iLID
Plasmid#190165PurposeExpresses EB3N-VVDfast-mCl3-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells.DepositorInsertEB3N-VVDfast-mClover3-iLID
UseTags-VVDfast-mClover3-iLIDExpressionMammalianMutation1-189 aa from EB3PromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSIN-TRE-EB3N-VVDfast-mCl3-iLID
Plasmid#190169PurposeDoxycycline-inducible lentivirus vector to express EB3N-VVDfast-mCl3-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60.DepositorInsertEB3N-VVDfast-mClover3-iLID
UseLentiviralTags-VVDfast-mClover3-iLIDExpressionMutation1-189 aa from EB3PromoterTREAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
mAC-POLR2A donor (Hygro)
Plasmid#124496PurposeA donor plasmid for tagging the N-terminus of human POLR2A with mAID-mCloverDepositorInsertPOLR2A homology arms and Hygro-P2A-mAID-mClover (POLR2A Human)
UseCRISPRTagsmAID-mCloverExpressionMutationPromoterAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTALYM3
Plasmid#47874PurposeDestination plasmid with mClover for Golden Gate reaction of TALE assemblyDepositorTypeEmpty backboneUseTALENTagsmCloverExpressionMammalianMutationPromoterCAGAvailable sinceOct. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Himar-137
Plasmid#185928PurposeHimar transposon delivery plasmid that delivers a green fluorescent mClover3 protein geneDepositorInsertmClover3
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only