1. K218Tc-3

   Plasmid

   #1300

   Depositor

   Susan Lindquist

   Article

   Schirmer et al Proc Natl Acad Sci U S A 2001 Jan 30;98(

   Insert

   mutant HSP104 (HSP104 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   K218T: A315T

   Promoter

   Available since

   Jan. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

These results may also be relevant.

1. pAB07 (CK2beta)

   Plasmid

   #27091

   Depositor

   David Litchfield

   Article

   Turowec et al Methods Enzymol. 2010;484:471-93.

   Insert

   CK2beta (CSNK2B Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 17, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pLC-Flag-UBE2G1 sg2R-P2A-Hygro

   Plasmid

   #124299

   Purpose

   Lentiviral vector for expression of Flag tagged UBE2G1-P2A-Hygro casette from a CMV promoter. UBE2G1 cDNA contains silent mutations to disrupt a sgRNA binding site.

   Depositor

   Eva Gottwein

   Article

   Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

   Insert

   UBE2G1 (UBE2G1 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   several silent mutations to disrupt sgRNA targeti…

   Promoter

   CMV

   Available since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pPN286

   Plasmid

   #91669

   Purpose

   Express sgRNA targeting human SLC45A1

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

   Insert

   SLC45A1 (SLC45A1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pPN287

   Plasmid

   #91670

   Purpose

   Express sgRNA targeting human SLC45A1

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

   Insert

   SLC45A1 (SLC45A1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV-DNAJC5-ΔC30 (Δ169-198)

   Plasmid

   #205722

   Purpose

   Mammalian expression of DNAJC5-ΔC30 (Δ169-198)

   Depositor

   Randy Schekman

   Article

   Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

   Insert

   DNAJC5 (DNAJC5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Δ169-198

   Promoter

   Available since

   Feb. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV-XPACK-DNAJC5 (L115R)

   Plasmid

   #205717

   Purpose

   Mammalian expression of XPACK-DNAJC5 (L115R)

   Depositor

   Randy Schekman

   Article

   Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

   Insert

   DNAJC5 (DNAJC5 Human)
   

   Use

   Tags

   XPACK (GCINSKRKD)

   Expression

   Mammalian

   Mutation

   L115R

   Promoter

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-XPACK-DNAJC5 (WT)

   Plasmid

   #205716

   Purpose

   Mammalian expression of XPACK-DNAJC5 (WT)

   Depositor

   Randy Schekman

   Article

   Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

   Insert

   DNAJC5 (DNAJC5 Human)
   

   Use

   Tags

   XPACK (GCINSKRKD)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV-DNAJC5-ΔC10 (Δ189-198)

   Plasmid

   #205720

   Purpose

   Mammalian expression of DNAJC5-ΔC10 (Δ189-198)

   Depositor

   Randy Schekman

   Article

   Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

   Insert

   DNAJC5 (DNAJC5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Δ189-198

   Promoter

   Available since

   Jan. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-DNAJC5-ΔC20 (Δ179-198)

   Plasmid

   #205721

   Purpose

   Mammalian expression of DNAJC5-ΔC20 (Δ179-198)

   Depositor

   Randy Schekman

   Article

   Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

   Insert

   DNAJC5 (DNAJC5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Δ179-198

   Promoter

   Available since

   Jan. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-DNAJC5-ΔC40 (Δ151-198)

    Plasmid

    #205723

    Purpose

    Mammalian expression of DNAJC5-ΔC40 (Δ151-198)

    Depositor

    Randy Schekman

    Article

    Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

    Insert

    DNAJC5 (DNAJC5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Δ151-198

    Promoter

    Available since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV-DNAJC5 (L115R)-HaloTag

    Plasmid

    #205711

    Purpose

    Mammalian expression of DNAJC5 (L115R)-HaloTag

    Depositor

    Randy Schekman

    Article

    Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

    Insert

    DNAJC5 (DNAJC5 Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    L115R

    Promoter

    Available since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. TFORF1094

    Plasmid

    #143465

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    HSFX2 (HSFX2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Lenti-guide-puro mCsnk2b - 1

    Plasmid

    #198505

    Purpose

    lentiviral stable expression of mCsnk2b gRNA 1

    Depositor

    Suzanne Pfeffer

    Article

    Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

    Insert

    mCsnk2b (Csnk2b Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Lenti-guide-puro mCsnk2b - 2

    Plasmid

    #198506

    Purpose

    lentiviral stable expression of mCsnk2b gRNA 2

    Depositor

    Suzanne Pfeffer

    Article

    Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

    Insert

    mCsnk2b (Csnk2b Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLC-Flag-SENP8 sg2R-P2A-Hygro

    Plasmid

    #124300

    Purpose

    Lentiviral vector for expression of Flag tagged SENP8-P2A-Hygro casette from a CMV promoter. SENP8 cDNA contains silent mutations to disrupt a sgRNA binding site.

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

    Insert

    SENP8 (SENP8 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    several silent mutations to disrupt sgRNA targeti…

    Promoter

    CMV

    Available since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFUPI hT5Cyp-H126Q

    Plasmid

    #80281

    Purpose

    Expresses human TRIM5 CypA fusion with H126Q mutation in CypA

    Depositor

    Jeremy Luban

    Article

    Neagu et al J Clin Invest. 2009 Oct;119(10):3035-47.

    Insert

    TRIM5 CypA fusion (TRIM5, PPIA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    H126Q mutation in CypA

    Promoter

    Available since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEGFP-hMC4R

    Plasmid

    #179315

    Purpose

    Express human melanocortin 4 receptor (GFP tagged) in mammalian cells

    Depositor

    Christian Gruber

    Article

    Muratspahic et al ACS Pharmacol Transl Sci. 2023 Sep 26;6(

    Insert

    melanocortin 4 receptor (MC4R Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MC4R-Tango

    Plasmid

    #66430

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    MC4R (MC4R Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLC-CUL4A K705R-P2A-Puro

    Plasmid

    #124305

    Purpose

    Lentiviral vector for expression of Nedd8 attachment mutant K705R CUL4A-P2A-Puro casette from a CMV promoter

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

    Insert

    CUL4A (CUL4A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    changed Lysine 705 to Arginine

    Promoter

    CMV

    Available since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HA-CK2alpha’ V67A/I175A (pJD24)

    Plasmid

    #37363

    Depositor

    David Litchfield

    Article

    Duncan et al Mol Cell Proteomics. 2008 Jun;7(6):1077-

    Insert

    CK2α' V67A/I175A (CSNK2A2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    V67A, I175A

    Promoter

    CMV

    Available since

    July 17, 2012

    Availability

    Academic Institutions and Nonprofits only