Addgene

We narrowed to 4,148 results for: 027

Showing: 281 - 300 of 4148 results

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pAAV-ZnF-hSyn-DIO-PSD95FingR-mGreenLantern

Plasmid

#200278

Purpose

Genetically encoded fluorescent probe for labelling endogenous excitatory synapses in vivo

Depositor

Peter Scheiffele

Article

Okur et al bioRxiv 2023

Insert

ZnF-FingR-PSD95-mGreenLantern

Use

AAV

Tags

mGreenLantern

Expression

Mammalian

Mutation

Promoter

human Synapsin

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-ZnF-hSyn-DIO-GPHNFingR-eGFP

Plasmid

#200279

Purpose

Genetically encoded fluorescent probe for labelling endogenous inhibitory synapses in vivo

Depositor

Peter Scheiffele

Article

Okur et al bioRxiv 2023

Insert

zFn-FingR-GPHN-eGFP

Use

AAV

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

human Synapsin

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pG12-SEAP

Plasmid

#170271

Purpose

Secreted alkaline phosphatase (SEAP) expression in mammalian cells. Reporter plasmid.

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

Insert

SEAP

Use

Tags

Expression

Mammalian

Mutation

Promoter

12xUAS with TATA-box minimal promoter

Available since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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TroponinT-GCaMP5-Zeo

Plasmid

#46027

Depositor

John Gearhart

Article

Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

Insert

GCaMP5-2A-Zeo

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TNNT2

Available since

July 24, 2013

Availability

Academic Institutions and Nonprofits only

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pBBK43 pHomL-TDH3p-GFPdropout-SSA1t-HomR (AmpR)

Plasmid

#179027

Purpose

Parent vector for markerless yeast integration cassettes with untagged genes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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PH-PLCd1-mNGx1

Plasmid

#202751

Purpose

expresses fluorescent protein-tagged lipid biosensor

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

PLCD1v2(1-170):mNG2 (PLCD1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pSWEET-bgaB

Plasmid

#210272

Purpose

Xylose-dependent expression system for tight and modulated expression of cloned genes in Bacillus subtilis.

Depositor

Eric Brown

Article

Bhavsar et al Appl Environ Microbiol. 2001 Jan;67(1):4

Insert

bgaB

Use

Tags

Expression

Bacterial

Mutation

Promoter

PxylA

Available since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-Pink Flamindo-p2a-bPAC

Plasmid

#202716

Purpose

Mammalian co-expression of soluble Pink Flamindo, a fluorescent cAMP indicator and soluble bPAC, a photoactivatable adenylyl cyclase

Depositor

Adam Cohen

Article

Xiang et al bioRxiv 2023

Inserts

Pink Flamindo
bPAC

Use

Lentiviral

Tags

10xHis and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-PIP5K1A

Plasmid

#202720

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

EGFP:PIP5K1A (PIP5K1A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Antares2 c-myc

Plasmid

#100027

Purpose

A BRET based CyOFP-teLuc-CyOFP fusion to further red-shift bioluminescence

Depositor

Huiwang Ai

Article

Yeh et al Nat Methods. 2017 Sep 4. doi: 10.1038/nm

Insert

Antares2

Use

Tags

c-myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 13, 2017

Availability

Academic Institutions and Nonprofits only

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HSA3_His

Plasmid

#202765

Purpose

human serum albumin design HSA3, in pET29b with C-terminal 6His-tag

Depositor

Sarel Fleishman

Article

Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

Insert

HSA3

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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TagBFP2-PIP4K2A

Plasmid

#202726

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mTagBFP2:PIP4K2A (PIP4K2A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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pBoxCDGC-KMet-EGFP

Plasmid

#140272

Purpose

L7Ae-dependent translational repression

Depositor

Hirohide Saito

Article

Saito et al Nat Chem Biol. 2010 Jan;6(1):71-8. doi:

Insert

BoxCD

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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BSA1_His

Plasmid

#202766

Purpose

bovine serum albumin design BSA1, in pET29b with C-terminal 6His-tag

Depositor

Sarel Fleishman

Article

Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

Insert

BSA1

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pMVS1111A:PhmtB-bgaB

Plasmid

#205998

Purpose

Conjugative shuttle vector for Escherichia coli and Methanothermobacter thermautotrophicus encoding beta-galactosidase

Depositor

Bastian Molitor

Article

Fink et al mBio. 2021 Dec 21;12(6):e0276621. doi: 1

Insert

thermostable beta-galactosidase

Use

Tags

Expression

Mutation

Promoter

PhmtB

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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iRFP670-PIP5K1A

Plasmid

#202749

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

iRFP670:PIP5K1A (PIP5K1A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pUC19-TgKI-MCS-p2A-tdTomato-polyA

Plasmid

#174027

Purpose

This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

Depositor

Michel Bagnat

Article

Levic et al Development. 2021 Sep 8. pii: 272090. do

Insert

p2A-tdTomato

Use

Zebrafish knock-in tagging

Tags

Expression

Mutation

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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HSA1_His

Plasmid

#202763

Purpose

human serum albumin design HSA1, in pET29b with C-terminal 6His-tag

Depositor

Sarel Fleishman

Article

Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

Insert

HSA1

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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GST-Pin1

Plasmid

#19027

Depositor

Michael Yaffe

Article

Yaffe et al Science. 1997 Dec 12. 278(5345):1957-60.

Insert

Pin1 (PIN1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 25, 2008

Availability

Academic Institutions and Nonprofits only

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pBbS5c-mCherry-AsLOV2*(543)

Plasmid

#202712

Purpose

Expresses an IPTG inducible mCherry fused to a blue light responsive degradation tag (LOVdeg)

Depositor

Mary Dunlop

Article

Tague et al Elife. 2024 Jan 25;12:RP87303. doi: 10.7

Insert

mCherry-AsLOV2*(543)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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