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Showing: 701 - 750 of 4441 results

  6. pUC19-Top-Toehold-mCherry

    Plasmid
    #87318
    Purpose
    Expresses the "Top" transcript of the Split-Broccoli system upstream of an RNA Toehold switch controlling mCherry.
    Depositor
    Donald Burke
    Article
    Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102
    Insert
    Split-Broccoli "Top" transcript upstream of an RNA Toehold (TS2_KS01) controlling mCherry translation.
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P70a
    Available since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    87318-plasmid-map-sequence-id-174668

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  9. pUC-dirTopo-P2A-aGFP

    Plasmid
    #61974
    Purpose
    A directional cloning vector of PCR-amplified cDNAs for mammalian expression
    Depositor
    Hiroshi Udo
    Inserts
    lacZ alpha
    EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    It contains silent mutations
    Promoter
    Available since
    Feb. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    61974-plasmid-map-sequence-id-101282

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  13. pUC18-mini-Tn7T-Gm-LacZ30

    Plasmid
    #65028
    Purpose
    GmR mini-Tn7 vector, for construction of B-galactosidase protein fusions, frame 3. See Depositor Comments below for information regarding the lacZ sequence.
    Depositor
    Herbert Schweizer
    Article
    Choi et al Nat Protoc. 2006;1(1):153-61.
    Type
    Empty backbone
    Use
    Tags
    lacZ
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 2, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    65028-plasmid-map-sequence-id-109988

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  16. Mishler2014-No-aptamer-controlluciferase-pUC19

    Plasmid
    #63856
    Purpose
    This pUC19 vector contains a no-aptamer control mRNA encoding luciferase gene. It should be used when testing AEB-TMR7 and AEB-TMR10 riboswitches (all on pUC19) to measure their non-specific ligand effect on luciferase expression.
    Depositor
    Howard Salis
    Article
    Espah Borujeni et al Nucleic Acids Res. 2015 Nov 30. pii: gkv
    Insert
    No-aptamer control mRNA
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    tac
    Available since
    Feb. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  23. pUC-T7-hTP53-P0~670-FLuc

    Plasmid
    #208640
    Purpose
    for in vitro transcription template preparation, insert T7 promoter, TP53 translation initiation site P0 to +670 and F-luc from pGL3-Basic, into pUC19. P0~670 is confusing, BLAST is recommended.
    Depositor
    Quanfu Ma
    Insert
    TP53, UTR and partial CDS (TP53 Human)
    Use
    Luciferase; In vitor transcription template
    Tags
    Firefly Luciferase (F-Luc) and T7 promoter
    Expression
    Mutation
    UTR and partial CDS in-frame with F-Luc
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208640-plasmid-map-sequence-id-418347

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  24. pUC-ODN-hsRPL3-CterTwStrep

    Plasmid
    #208644
    Purpose
    for long single strand DNA donnor preparation for Twin Strep tag knock-in at C ter of human RPL3. Digest with nt.BspQI and nb.BsrDI to get ssDNA. This plasmid will cause re-edit and fail knock-in.
    Depositor
    Quanfu Ma
    Insert
    Twin Strep tag with RPL3 genome HomoArm (RPL3 Human, Synthetic)
    Use
    CRISPR; Ssdna donnor preparation
    Tags
    Twin Strep
    Expression
    Mutation
    Promoter
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208644-plasmid-map-sequence-id-418358

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  29. pVH1002-Tier2(pUC ori)

    Plasmid
    #169600
    Purpose
    Tier-2 expression vector (A1-pA::A2-pA::A3-pA). The tier-2 expression vector consists of a custom MCS cassette containing the three Tier-1 compatible acceptor cassettes (A1, A2, and A3) each with its own non-homologous polyadenylation (pA) signals in a minimal backbone (AmpR and pUC ori).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    169600-plasmid-map-sequence-id-366610

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  31. pUC19 KGA HA5'-mKO2-3'

    Plasmid
    #110406
    Purpose
    Homology donor for glutaminase KGA isoform knock-in, mKO2
    Depositor
    Sandra Martha Gomes Dias
    Insert
    GLS glutaminase (GLS Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    none
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    110406-plasmid-map-sequence-id-218847

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  38. pUC57 DYSF, alt exon 1, 17

    Plasmid
    #87893
    Purpose
    research of alternative isoforms of the protein dysferlin
    Depositor
    Jain Foundation
    Insert
    DYSF, alt exon 1, 17 (DYSF Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    alternative isoforms, see comments
    Promoter
    Available since
    April 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    87893-plasmid-map-sequence-id-174238

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  39. pUC57 DYSF, alt exon 1a

    Plasmid
    #87902
    Purpose
    research of alternative isoforms of the protein dysferlin
    Depositor
    Jain Foundation
    Insert
    DYSF, alt exon 1a (DYSF Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    alternative isoforms, see comments
    Promoter
    Available since
    March 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    87902-plasmid-map-sequence-id-174261

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  40. pUC57 DYSF, alt exon 1a, 17

    Plasmid
    #87900
    Purpose
    research of alternative isoforms of the protein dysferlin
    Depositor
    Jain Foundation
    Insert
    DYSF, alt exon 1a, 17 (DYSF Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    alternative isoforms, see comments
    Promoter
    Available since
    March 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    87900-plasmid-map-sequence-id-174254

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  41. pUC57 DYSF, alt exon 1a, 5a

    Plasmid
    #87898
    Purpose
    research of alternative isoforms of the protein dysferlin
    Depositor
    Jain Foundation
    Insert
    DYSF, alt exon 1a, 5a (DYSF Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    alternative isoforms, see comments
    Promoter
    Available since
    March 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    87898-plasmid-map-sequence-id-174249

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  42. pUC57 DYSF, alt exon 1a, 5a, 17

    Plasmid
    #87896
    Purpose
    research of alternative isoforms of the protein dysferlin
    Depositor
    Jain Foundation
    Insert
    DYSF, alt exon 1a, 5a, 17 (DYSF Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    alternative isoforms, see comments
    Promoter
    Available since
    March 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    87896-plasmid-map-sequence-id-174247

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  43. pUC57 DYSF, alt exon 1, 5a

    Plasmid
    #87890
    Purpose
    research of alternative isoforms of the protein dysferlin
    Depositor
    Jain Foundation
    Insert
    DYSF, alt exon 1, 5a (DYSF Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    alternative isoforms, see comments
    Promoter
    Available since
    March 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    87890-plasmid-map-sequence-id-174218

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  44. pUC57 DYSF, alt exon 1, 5a, 17

    Plasmid
    #87888
    Purpose
    research of alternative isoforms of the protein dysferlin
    Depositor
    Jain Foundation
    Insert
    DYSF, alt exon 1, 5a, 17 (DYSF Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    alternative isoforms, see comments
    Promoter
    Available since
    March 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    87888-plasmid-map-sequence-id-174215

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  45. pUC-dirTopo-AU1-aGFP

    Plasmid
    #61973
    Purpose
    A directional cloning vector of PCR-amplified cDNAs for mammalian expression
    Depositor
    Hiroshi Udo
    Inserts
    lacZ alpha
    EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    It contains silent mutations
    Promoter
    Available since
    Feb. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    61973-plasmid-map-sequence-id-101284

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Showing: 701 - 750 of 4441 results