Addgene

We narrowed to 3,173 results for: ado

Showing: 561 - 600 of 3173 results

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pAAV-UAS-CITRINE

Plasmid

#104839

Purpose

SPARK reporter gene

Depositor

Alice Ting

Article

Kim et al Elife. 2017 Nov 30;6. doi: 10.7554/eLife

Insert

Citrine

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

UAS

Available since

Jan. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
TRE-mCherry-p2A-eNpHR3.0-TS

Plasmid

#158703

Purpose

Reporter construct

Depositor

Alice Ting

Article

Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

Insert

mCherry-p2A-eNpHR3.0-TS

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-UAS-Luciferase

Plasmid

#104840

Purpose

SPARK reporter gene

Depositor

Alice Ting

Article

Kim et al Elife. 2017 Nov 30;6. doi: 10.7554/eLife

Insert

Luciferase

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

UAS

Available since

Jan. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX208-beta-arrestin2-HA-TEV219

Plasmid

#104846

Purpose

b2AR-SPARK protease component

Depositor

Alice Ting

Article

Kim et al Elife. 2017 Nov 30;6. doi: 10.7554/eLife

Insert

beta-arrestin2-HA-TEV219

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pFETCh_ZBTB1

Plasmid

#128235

Purpose

Donor vector for 3' FLAG tag of human ZBTB1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZBTB1 Homology arms

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mutation

Promoter

Available since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbA6c_PsulA_sfGFP

Plasmid

#224587

Purpose

SOS response reporter

Depositor

Ophelia Venturelli

Article

Cheng et al Mol Syst Biol. 2023 Mar 9;19(3):e11406.

Insert

sfGFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PsulA

Available since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBPGUw

Plasmid

#17575

Depositor

Gerald Rubin

Article

Pfeiffer et al Tools for neuroanatomy and neurogenetics

Insert

GAL4 (GAL4 Budding Yeast)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 28, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
PX458_RAD21_1

Plasmid

#64057

Purpose

Expresses gRNA against human RAD21 along with Cas9 with 2A GFP

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
PX458_CREB1_1

Plasmid

#64939

Purpose

Expresses gRNA against human CREB1 along with Cas9 with 2A GFP

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
TRE-mCherry-p2A-Chrimson-TS

Plasmid

#158702

Purpose

Reporter construct

Depositor

Alice Ting

Article

Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

Insert

TRE-mcherry-p2A-Chrimson-TS

Use

Synthetic Biology

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-HA-rat Nrxn1alpha

Plasmid

#58266

Purpose

Expression plasmid for HA-tagged rat Neurexin 1alpha

Depositor

Peter Scheiffele

Article

Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.

Insert

Nrxn1alpha (Nrxn1 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

see comments

Promoter

CAG

Available since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-TREX1

Plasmid

#27218

Depositor

Judy Lieberman

Article

Yan et al Nat Immunol. 2010 Nov . 11(11):1005-13.

Insert

TREX1 (TREX1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

n/a

Promoter

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only
pHC79

Plasmid

#167551

Purpose

Backbone plasmid to pPM1001, pPM1002, pPM1003, pPM2212 and pPM2213

Depositor

Renato Morona

Article

Manning et al Infect Immun. 1986 Aug;53(2):272-7. doi:

Type

Empty backbone

Use

Cosmid

Tags

Expression

Mutation

Promoter

Available since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pFPGW-UAS-Citrine

Plasmid

#125232

Purpose

For generating UAS-Citrine lentivirus

Depositor

Alice Ting

Article

Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

Insert

UAS-Citrine

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

UAS

Available since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
DJ-1 pET15b

Plasmid

#60678

Purpose

Recombinant protein expression of Park7

Depositor

Mark Wilson

Article

Lakshminarasimhan et al Biochemistry. 2008 Feb 5;47(5):1381-92.

Insert

DJ-1 (PARK7 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 4, 2014

Availability

Academic Institutions and Nonprofits only
pBPGw

Plasmid

#17574

Depositor

Gerald Rubin

Article

Pfeiffer et al Tools for neuroanatomy and neurogenetics

Insert

GAL4 (GAL4 Budding Yeast)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 28, 2008

Availability

Academic Institutions and Nonprofits only
pDWJ27

Plasmid

#207604

Purpose

GFP-BIB expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-BIB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
PX458_CEBPB_1

Plasmid

#64036

Purpose

Expresses gRNA against human CEBPB along with Cas9 with 2A GFP

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

July 14, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
PX458_CEBPB_2

Plasmid

#64047

Purpose

Expresses gRNA against human CEBPB along with Cas9 with 2A GFP

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-BLZinCh-1

Plasmid

#85082

Purpose

mammalian vector for the expression of a dual read-out BRET-FRET sensor for intracellular Zn2+ imaging

Depositor

Maarten Merkx

Article

Aper et al ACS Chem Biol. 2016 Oct 21;11(10):2854-2

Insert

BL-ZinCh-1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJS65

Plasmid

#207587

Purpose

GFP-TonB expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-TonB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBluescript PMS1

Plasmid

#16457

Depositor

Bert Vogelstein

Article

Nicolaides et al Nature. 1994 Sep 1. 371(6492):75-80.

Insert

PMS1 (PMS1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
PX458_CREB1_2

Plasmid

#64940

Purpose

Expresses gRNA against human CREB1 along with Cas9 with 2A GFP

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
PX458_GABPA_2

Plasmid

#64255

Purpose

Expresses gRNA against human GABPA along with Cas9 with 2A GFP

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pJS61

Plasmid

#207585

Purpose

GFP-TolA expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-TolA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLSG (stra8)

Plasmid

#21619

Depositor

David Garbers

Article

Dann et al Stem Cells. 2008 Nov . 26(11):2928-37.

Insert

Stra8 promoter (Stra8 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 25, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
PX458_GABPA_1

Plasmid

#64254

Purpose

Expresses gRNA against human GABPA along with Cas9 with 2A GFP

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-Map7emtb-Kif5bmotor

Plasmid

#46077

Depositor

Edgar Gomes

Article

Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

Insert

Kif5bmotor-Map7emtb

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pNVLTv2

Plasmid

#169245

Purpose

Generic suicide vector for knockouts and integrations

Depositor

Brian Pfleger

Article

Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
PX458_ATF1_1

Plasmid

#64690

Purpose

Expresses gRNA against human ATF1 along with Cas9 with 2A GFP

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
dnCEBP: pLenti-TRE-A-C/EBP-PGK-rtTAA-2A-Puro

Plasmid

#177201

Purpose

encoding a dominant negative form of CEBP under inducible promoter

Depositor

Markus Covert

Article

Kudo et al bioRxiv (2022)

Insert

dnCEBP (A-C/EBP)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Farnesylated SE pcDNA3.1

Plasmid

#79666

Purpose

Expresses super ecliptic A227D in mammalian cell membranes

Depositor

Bradley Baker

Article

Kang et al Sci Rep. 2016 Apr 4;6:23865. doi: 10.103

Insert

superEcliptic pHluorin

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pFETCh_SIX4_iso1

Plasmid

#128238

Purpose

Donor vector for 3' FLAG tag of human SIX4_iso1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

SIX4_iso1 Homology arms

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mutation

Promoter

Available since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-stl

Plasmid

#51721

Purpose

conditional gene knockdowns in Trypanosoma brucei

Depositor

Arthur Gunzl

Article

Brandenburg et al EMBO J. 2007 Nov 28;26(23):4856-66. Epub

Type

Empty backbone

Use

Conditional gene knockdown in t. brucei

Tags

Expression

Mutation

Promoter

T7 (2X)

Available since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-Map7-1333-end

Plasmid

#46073

Depositor

Edgar Gomes

Article

Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

Insert

Map7D1-1333-end

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-Map7-856-1351

Plasmid

#46072

Depositor

Edgar Gomes

Article

Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

Insert

Map7D1-856-1351

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-Map7-1783-end

Plasmid

#46074

Depositor

Edgar Gomes

Article

Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

Insert

Map7D1-1783-end

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
DGP104- pSP64 Poly(A)700

Plasmid

#70098

Purpose

an RNA spike-in with similar GC content to S. cerevisiae mRNAs, for in vivo measurement of RNA kinetics

Depositor

David Gresham

Article

Neymotin et al RNA. 2014 Oct;20(10):1645-52. doi: 10.12

Insert

700bp insert from B. subtilis

Use

In vitro transcription

Tags

Expression

Mutation

Promoter

SP6

Available since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only
DGP105- pSP64 Poly(A)900

Plasmid

#70100

Purpose

an RNA spike-in with similar GC content to S. cerevisiae mRNAs, for in vivo measurement of RNA kinetics

Depositor

David Gresham

Article

Neymotin et al RNA. 2014 Oct;20(10):1645-52. doi: 10.12

Insert

900bp insert from B. subtillis

Use

In vitro transcription

Tags

Expression

Mutation

Promoter

SP6

Available since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only
DGP106- pSP64 Poly(A)1200

Plasmid

#70101

Purpose

an RNA spike-in with similar GC content to S. cerevisiae mRNAs, for in vivo measurement of RNA kinetics

Depositor

David Gresham

Article

Neymotin et al RNA. 2014 Oct;20(10):1645-52. doi: 10.12

Insert

1200bp insert from B. subtillis

Use

In vitro transcription

Tags

Expression

Mutation

Promoter

SP6

Available since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

Showing: 561 - 600 of 3173 results

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