171,009 results

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pDx_mScarlet-I3

Plasmid

#189757

Purpose

Dual expression vector for bacteria and mammalian cells producing mScarlet-I3 red fluorescent protein

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet-I3

Tags

6xHis

Expression

Bacterial and Mammalian

Promoter

CMV & Rha

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-puro_GCaMP6s

Plasmid

#164589

Purpose

Stable integration and expression of GCaMP6s in mammalian cells

Depositor

Joseph Hyser

Article

Chang-Graham et al Sci Rep. 2019 Jul 25;9(1):10822. doi: 10

Insert

GCaMP6s

Use

Lentiviral

Tags

6xHis, T7 tag, and Xpress tag

Expression

Mammalian

Promoter

CMV

Available Since

March 3, 2021

Availability

Academic Institutions and Nonprofits only

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pKC105_pLenti_TetON_PABPC1_INT

Plasmid

#242110

Purpose

Tet- or Dox-inducible expression of PABPC1 fused to INT (human PARP4 fragment)

Depositor

Fei Chen

Article

Chao et al Science. 2026 Jan 15:eadz9353. doi: 10.1

Insert

PAB4L, PARP4 fragment (PABPC1 Human)

Use

Lentiviral

Mutation

PABPC1 aa 1-370, PARP4 aa 1562-1724

Promoter

TRE3G

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pJRH-1362 scFv entry plasmid

Plasmid

#201912

Purpose

Entry vector for cloning single chain variable fragments displayed on the human CD8a hinge and transmembrane domain (CAG promoter)

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

Stuffer sequence (drop out for scFv cloning) + CD8 hinge and transmembrane domain

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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Lifeact-mCherry

Plasmid

#193300

Purpose

mammalian transient expression - expression of Lifeact-mCherry

Depositor

Klaus Hahn

Article

Herron et al Nat Commun. 2022 Jul 27;13(1):4363. doi:

Insert

Lifeact

Tags

mCherry

Expression

Mammalian

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ExRai-AMPKAR(T/A)

Plasmid

#192447

Purpose

Non-phosphorylatable negative-control mutant of ExRai-AMPKAR.

Depositor

Jin Zhang

Article

Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

Insert

ExRai-AMPKAR(T/A)

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

Expression

Mammalian

Mutation

Thr 6 in AMPK substrate mutated to Ala.

Promoter

CMV

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pRS406-Padh-OsTIR1(F74G)

Plasmid

#247604

Purpose

Expression of OsTIR1

Depositor

Sebastian Schuck

Article

Hubbe et al Mol Biol Cell. 2026 Feb 1;37(2):mr2. doi

Insert

OsTIR1

Expression

Yeast

Mutation

F74G

Promoter

ADH1

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pUK21

Plasmid

#49788

Purpose

E. coli cloning vector (KanR, high copy, blue/white selection, M13 IR)

Depositor

Joachim Messing

Article

Vieira et al Gene. 1991 Apr;100:189-94.

Type

Empty backbone

Use

Cloning vector

Promoter

lacZ

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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DLX2-hygro

Plasmid

#97330

Purpose

Generating lentiviruses to express Dlx2 and hygromycin resistance

Depositor

Marius Wernig

Article

Yang et al Nat Methods. 2017 May 15. doi: 10.1038/n

Insert

DLX2 (Dlx2 Mouse)

Use

Lentiviral

Promoter

TetO

Available Since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-PEmax-P2A-hMLH1dn

Plasmid

#174828

Purpose

Mammalian expression of SpCas9 PEmax prime editor with P2A-human MLH1dn (codon optimized)

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

PEmax-P2A-hMLH1dn

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Mutation

Detailed in manuscript; deletion of residues 754-…

Promoter

CMV

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-LIC

Plasmid

#26094

Purpose

SGC Empty backbone for bacterial expression

Depositor

Cheryl Arrowsmith

Type

Empty backbone

Tags

6xHis and Thrombin cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available Since

Aug. 20, 2010

Availability

Industry, Academic Institutions, and Nonprofits

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pLD-puro-MTS-EGFP

Plasmid

#190270

Purpose

Lentiviral conditional expression of mitochondrial-targeted eGFP in mammalian cells

Depositor

Christian Münch

Article

Michaelis et al Bio Protoc. 2022 Dec 20;12(24):e4578. do

Insert

MTS-EGFP

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

Tight TRE promoter

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pGFPGUSPlus

Plasmid

#64401

Purpose

Binary plant vector expressing GFP and GUS, both driven by a 35S promoter

Depositor

Claudia Vickers

Article

Vickers et al Biotechnol Lett. 2007 Nov;29(11):1793-6.

Inserts

GFP
GUSPlus

Use

Binary vector for plant transformation

Tags

His

Expression

Plant

Mutation

S65T

Promoter

35S

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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pHR-UCOE-SFFV-Zim3-dCas9-P2A-mCherry

Plasmid

#188779

Purpose

dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Zim3-dCas9

Use

Lentiviral

Tags

Zim3 KRAB-NLS fusion

Promoter

SFFV

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pmTurquoise2-N1

Plasmid

#60561

Purpose

Expresses the cyan fluorescent protein variant mTurquoise2, can be used to create fusion proteins

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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scramble shRNA

Plasmid

#1864

Purpose

3rd gen lentiviral negative control vector containing scrambled shRNA

Depositor

David Sabatini

Article

Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

Insert

scramble

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

April 5, 2006

Availability

Academic Institutions and Nonprofits only

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SigmaR1-mNeonGreen

Plasmid

#226566

Purpose

Encodes SigmaR1 protein labeled with mNeonGreen

Depositor

Gia Voeltz

Article

Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

Insert

SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)

Tags

mNeonGreen

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pInducer20

Plasmid

#44012

Purpose

Tet-inducible lentiviral vector for ORF expression

Depositor

Stephen Elledge

Article

Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

Type

Empty backbone

Use

Lentiviral

Tags

HA

Expression

Mammalian

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2-mCherry

Plasmid

#99154

Purpose

Lentiviral vector encoding sgRNA cloning site + hSpCAS9-P2A-mCherry.

Depositor

Agata Smogorzewska

Inserts

Cas9
mCherry

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EFS-NS

Available Since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pLentiFUCCI(CA)5

Plasmid

#223176

Purpose

Lentiviral fluorescent ubiquitination-based cell cycle indicator (FUCCI)

Depositor

Neville Sanjana

Article

Liang et al Cell. 2024 Dec 26;187(26):7637-7654.e29.

Insert

FUCCI(CA)5

Use

Lentiviral

Tags

AzaleaB5-hCdt1(1/100)Cy(-)_P2A_h2-3-hGem(1/110)

Expression

Mammalian

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pBbB19k-GFP

Plasmid

#126135

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

GFPuv

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23105

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pLS-SceI

Plasmid

#137725

Purpose

Lentivirus reporter assay plasmid that contains a I-SceI site and a EGFP reporter gene.

Depositor

Nadav Ahituv

Article

Gordon et al Nat Protoc. 2020 Aug;15(8):2387-2412. do

Type

Empty backbone

Use

Lentiviral

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pHIV-EGFP

Plasmid

#21373

Purpose

self-inactivating lentiviral plasmid for co-expression of your gene of interest and EGFP

Depositor

Bryan Welm, Zena Werb

Article

Welm et al Cell Stem Cell. 2008 Jan 10. 2(1):90-102

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

June 19, 2009

Availability

Academic Institutions and Nonprofits only

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pZR071_SFFV-dCas9-mCherry-KRAB

Plasmid

#180264

Purpose

Lentiviral expression vector for dCas9-KRAB in mammalian cells

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

KRAB and mCherry

Expression

Mammalian

Mutation

Nuclease dead Cas9

Promoter

SFFV

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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EdTP

Plasmid

#24311

Depositor

Roel Nusse

Article

Fuerer et al PLoS One. 2010 . 5(2):e9370.

Insert

EF1alpha-dnhTcf4 // SV40-PuroR (TCF7L2 Human)

Use

Lentiviral

Tags

FLAG

Mutation

31 first amino acids of hTcf4 have been deleted.

Available Since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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mEGFP-NbALFA

Plasmid

#159986

Purpose

For the expresson and purification of the EGFP-tagged anti-ALFA tag nanobody (NbALFA)

Depositor

Motoyuki Hattori

Article

Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

Insert

mEGFP-NbALFA

Tags

8histidine-HRV3C protease cleavage site

Expression

Bacterial

Mutation

3GS linker between mEGFP and NbALFA

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pLV hUbC-VP64 dCas9 VP64-T2A-GFP

Plasmid

#59791

Purpose

Co-expresses human optimized S. pyogenes dCas9 fused to two copies of VP64 and GFP

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Insert

humanized VP64 dead Cas9 VP64 T2A GFP

Use

CRISPR and Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

D10A and H840A

Available Since

Oct. 23, 2014

Availability

Academic Institutions and Nonprofits only

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pCRY2PHR-mCherryN1

Plasmid

#26866

Purpose

Expresses CRY2(1-498)-mCherry fusion for use in light-inducible protein interaction modules

Depositor

Chandra Tucker

Article

Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

Insert

CRY2PHR (CRY2 Mustard Weed)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
tetO.Nfib.Hygro

Plasmid

#117271

Purpose

Expresses Nfib and hygromycin resistance gene under control of tetO sequence.

Depositor

Henrik Ahlenius

Article

Canals et al Nat Methods. 2018 Sep;15(9):693-696. doi

Insert

Nfib (Nfib Mouse)

Use

Lentiviral

Tags

IRES and hygromycin resistant cassette (C termina…

Expression

Mammalian

Promoter

tet-on

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pZR112_Lenti-SFFV-mCherry-2A-dCas9-VP64

Plasmid

#180263

Purpose

Lentiviral expression vector for dCas9-VP64 in mammalian cells

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Insert

dCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

Nuclease dead Cas9

Promoter

SFFV

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-ADAM10-HA

Plasmid

#65106

Purpose

Expression of HA tagged ADAM10 in mammalian cells

Depositor

Axel Ullrich

Article

Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

Insert

ADAM10 (ADAM10 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pBluescript II NLS-MC-EGFP-tdTomato (pBET2)

Plasmid

#240411

Purpose

This plasmid facilitates the assessment of MMR proficiency in human living cells

Depositor

Isao Kuraoka

Article

Takedachi et al Mutat Res. 2022 Jan-Jun;824:111779. doi:

Insert

NLS-EGFP fusion protein

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
eeBxb1_IVT

Plasmid

#222342

Purpose

Plasmid for evolved and engineered Bxb1 mRNA in vitro transcription template

Depositor

David Liu

Article

Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

Insert

Codon-optimized evolved and engineered Bxb1

Use

Template for in vitro transcription

Promoter

T7 (inactivated)

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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Hsp90 HA

Plasmid

#22487

Depositor

William Sessa

Article

Garcia-Cardena et al Nature. 1998 Apr 23. 392(6678):821-4.

Insert

Hsp90 HA (HSP90AB1 Human)

Tags

HA

Expression

Mammalian

Available Since

May 20, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pTMN140 - UCOE-SFFV-VPH-XTEN-dCas9-IRES2-BFP-T2A-Blast

Plasmid

#238166

Purpose

Express VPH-dCas9 and BFP-T2A-BlasticidinR in mammalian cells. Lentiviral

Depositor

Thomas Norman

Article

Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

Insert

VPH-dCas9

Use

CRISPR and Lentiviral

Tags

mTagBFP2

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
tetO.Sox9.Puro

Plasmid

#117269

Purpose

Expresses Sox9 and puromycin resistance gene under control of tetO sequence.

Depositor

Henrik Ahlenius

Article

Canals et al Nat Methods. 2018 Sep;15(9):693-696. doi

Insert

Sox9 (Sox9 Mouse)

Use

Lentiviral

Tags

T2A and puromycin resistant cassette (C terminal …

Expression

Mammalian

Promoter

tet-on

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-GfaABC1D-MCS--4x6T-WPRE

Plasmid

#196417

Purpose

Empty vector for astrocyte-selective expression in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

Depositor

Stanley Thomas Carmichael

Article

Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

Type

Empty backbone

Use

AAV; Astrocyte-selective

Expression

Mammalian

Promoter

GfaABC1D

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCre

Plasmid

#236212

Purpose

Arabinose-inducible Cre recombinase

Depositor

Matthew Waldor

Article

Basta et al Nat Microbiol. 2025 Mar 27. doi: 10.1038

Insert

Cre recombinase

Expression

Bacterial

Promoter

araBAD promoter

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRR-MR-5Z

Plasmid

#23059

Depositor

Charles Miller

Article

Miller CA et al Plasmid. 2010 Mar;63(2):73-8. doi: 10.10

Insert

MR (NR3C2 Human)

Expression

Yeast

Mutation

N528H, Q814R, H993L

Available Since

Jan. 27, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-Lyn-PlcR

Plasmid

#181830

Purpose

Genetically encoded FRET-based sensor for monitoring plasma membrane PI(4,5)P2 dynamics in living cells.

Depositor

Jin Zhang

Article

Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

Insert

Lyn-PlcR

Tags

ECFP, EYFP, and N-terminal targeting sequence fro…

Expression

Mammalian

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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171,009 results