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Addgene
Showing: 281 - 300 of 10427 results

  1. pAV10.KN

    Plasmid
    #63209
    Purpose
    pCACS backbone (Amp), cloNAT, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    63209-plasmid-map-sequence-id-115799

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  2. pAV10.K5

    Plasmid
    #63210
    Purpose
    pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI or BciVI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    63210-plasmid-map-sequence-id-104911

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  3. pAV10.HO6

    Plasmid
    #63211
    Purpose
    pCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into HO locus (NotI or BciVI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    63211-plasmid-map-sequence-id-115802

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  4. pAV10.K4

    Plasmid
    #63212
    Purpose
    pCACS backbone (Amp), TRP1, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into chrIXR: 387328-388330.
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    63212-plasmid-map-sequence-id-104913

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  5. pAV10

    Plasmid
    #63213
    Purpose
    pUC19 backbone (Amp), contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. NotI or FseI sites for TU digest.
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    63213-plasmid-map-sequence-id-113915

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  12. pTara:500

    Plasmid
    #60717
    Purpose
    Expresses T7 RNAP from the arabinose inducible promoter PBAD with a RBS strength of 500 from the Salis RBS calculator. Also includes a constitutive araC ORF
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Insert
    T7 RNAP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD
    Availability
    Academic Institutions and Nonprofits only
    60717-plasmid-map-sequence-id-97574

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  13. pTSara-N

    Plasmid
    #60721
    Purpose
    Expresses the N-terminal fragment of split T7 RNAP split a position 179. Insert is driven by PBAD. Contains a constitutive araC ORF
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Insert
    Residues 1-179 of T7 RNAP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Only amino acids 1-179 of T7 RNAP
    Promoter
    PBAD
    Availability
    Academic Institutions and Nonprofits only
    60721-plasmid-map-sequence-id-97707

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  14. pTSara-C

    Plasmid
    #60722
    Purpose
    Expresses the C-terminal fragment of split T7 RNAP split a position 179. Insert is driven by the arabinose inducible promoter PBAD. Contains a constitutive araC ORF
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Insert
    Residues 180-880 of T7 RNAP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Only amino acids 180-880 of T7 RNAP
    Promoter
    PBAD
    Availability
    Academic Institutions and Nonprofits only
    60722-plasmid-map-sequence-id-97708

    Enlarge

  15. pTSara-Q758C

    Plasmid
    #60724
    Purpose
    Expresses both fragments of T7 RNAP split at position 179. Both fragments are driven by PBAD. The C-terminal fragment has the point mutation Q758C. Contains a constitutive araC ORF
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Inserts
    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Residues 1-180 of split T7 RNAP and Residues 180-…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    60724-plasmid-map-sequence-id-97710

    Enlarge

  16. pTSara-R756S

    Plasmid
    #60725
    Purpose
    Expresses both fragments of T7 RNAP split at position 179. Both fragments are driven by PBAD. The C-terminal fragment has the point mutation R756S. Contains a constitutive araC ORF
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Inserts
    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Residues 1-180 of split T7 RNAP and Residues 180-…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    60725-plasmid-map-sequence-id-97713

    Enlarge

  17. pTSara-N748D

    Plasmid
    #60726
    Purpose
    Expresses both fragments of T7 RNAP split at position 179. Both fragments are driven by PBAD. The C-terminal fragment has the point mutation N748D. Contains a constitutive araC ORF
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Inserts
    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Residues 1-180 of split T7 RNAP and Residues 180-…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    60726-plasmid-map-sequence-id-97714

    Enlarge

  18. pTSara-R756K

    Plasmid
    #60727
    Purpose
    Expresses both fragments of T7 RNAP split at position 179. Both fragments are driven by PBAD. The C-terminal fragment has the point mutation R756K. Contains a constitutive araC ORF
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Inserts
    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Residues 1-180 of split T7 RNAP and Residues 180-…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    60727-plasmid-map-sequence-id-97715

    Enlarge

  19. pTSlb-wt

    Plasmid
    #60728
    Purpose
    Expresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD.
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Inserts
    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Residues 1-180 of split T7 RNAP and Residues 180-…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    60728-plasmid-map-sequence-id-99370

    Enlarge

  20. pTSlb-Q758C

    Plasmid
    #60729
    Purpose
    Expresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations Q758C
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Inserts
    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Residues 1-180 of split T7 RNAP and Residues 180-…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    60729-plasmid-map-sequence-id-99392

    Enlarge

Showing: 281 - 300 of 10427 results