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Plasmid#117768PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertTagRFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT2KXIG (Tol2)-tp63:ikk1KD:GFP
Plasmid#105645PurposeKinase-deleted Ikk1(alpha) expression in basal keratinocytesDepositorInserttp63:ikk1KD:GFP (chuk Zebrafish)
UseTagsGFPExpressionMammalianMutationp63 promoter + ikk1 (kinase domain deleted) + GFPPromoterAvailable sinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;tmem88aCE1-P1Egfp
Plasmid#90148Purposecontains tmem88a enhancer sequence, and tmem88a specific gene promoter driving expression of eGFPDepositorInserttmem88aE1P1Egfp (tmem88a Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
UAS::PTPRFa.1-DN-GFP
Plasmid#37110DepositorInsertUAS::PTPRFa.1-DN-GFP (ptprf Zebrafish)
UseTol2 gateway expression vectorTagsEGFPExpressionMutationdeleted amino acids 1326-1894PromoterUAS-E1bAvailable sinceJuly 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMTB2-cytoBirA-2A-mCherry_Ras
Plasmid#80066PurposeBeta-actin2 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialMutationPromoterBeta-actin2Available sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCK072 p5E 147bp exorh:EGFP
Plasmid#195950PurposeGateway p5E vector with reporterDepositorInsertexorh minimal regulatory elements and EGFP
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
UAS::PTPRFb-DN-GFP
Plasmid#37111DepositorInsertUAS::PTPRFb-DN-GFP (ptprfb Zebrafish)
UseTol2 gateway expression vectorTagsEGFPExpressionMutationDeleted aa1386-1909 corresponding to phosphatase …Promoter10xUASAvailable sinceJuly 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
LR (bact: HyPer)
Plasmid#162069PurposeUsed for the diffuse visualization of H2O2 production in cells.DepositorInsertbeta actin 2 (actb2 Zebrafish)
UseGateway donor vectorTagsHyPerExpressionMutationPromoterT3Available sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
374 pME-ets1a
Plasmid#49000Purposefull length coding sequence for zebrafish ets1a gene in gateway middle entry vectorDepositorInsertv-ets erythroblastosis virus E26 oncogene homolog 1a (ets1 Zebrafish)
UseGateway middle entryTagsExpressionMutationPromoterAvailable sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
ZE25 Stbm-pCS2+
Plasmid#17067DepositorInsertStbm (vangl2 Zebrafish)
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;lmo2P1Egfp
Plasmid#90162Purposecontains lmo2 specific gene promoter driving expression of eGFPDepositorInsertlmo2P1Egfp (lmo2 Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGTag-eGFP-CAAX-B-actin
Plasmid#117816PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInserteGFP
UseSynthetic BiologyTagsCAAXExpressionMutationPromoterAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT2-bactin:QF2,crybb:CFP
Plasmid#125697PurposeExpresses QF2 ubiquitously in zebrafish, with selectable marker CFP in eye lensDepositorInsertbactin:QF2
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-Stop-cmlc2-eGFP
Plasmid#117776PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsert3x3 Stop sequence
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
Danio rerio cryabb
Plasmid#47665PurposeRecombinant protein productionDepositorInsertAlpha B-crystallin (cryabb Zebrafish)
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-Cre-gcry-eGFP
Plasmid#117791PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertCre
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
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Zebrafish ArcLight Q175
Plasmid#53616PurposeGenetically encoded voltage sensor ArcLight with improved kineticsDepositorInsertZebrafish ArcLight-Q175 (tpte Synthetic, Zebrafish, Aequorea victoria)
UseTagsExpressionMammalianMutationDr-VSD contains R153Q mutation and an amino acid …PromoterCMVAvailable sinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
actc1b-lamp1-mKate2
Plasmid#109507PurposeMuscle specific zebrafish expression of mKate2 fused to lamp1DepositorInsertlamp1 (lamp1b Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only