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pcDNA3.1-Capri(1-10)

Plasmid

#165142

Purpose

Expresses Capri(1-10) in mammalian cells

Depositor

Daichi Kamiyama

Article

Tamura et al Commun Biol. 2021 Feb 26;4(1):257. doi:

Insert

Capri(1-10)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 8, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP

Plasmid

#165830

Purpose

EGFP green fluorescent protein CDS

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

EGFP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pOSV00170

Plasmid

#222934

Purpose

Constitutive GFP expression for Bacillus subtilis

Depositor

Ophelia Venturelli

Article

Cheng et al Nat Commun. 2023 Apr 10;14(1):2001. doi:

Insert

GFP(Sp)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Phyperspank

Available since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCR8-attL5-pegRNA-attR3

Plasmid

#213054

Purpose

Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL5 and attR3

Depositor

Bing Yang

Article

Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

Insert

sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pcDuex2-rsGreenF-E

Plasmid

#205752

Purpose

Expression of rsGreenF-E in mamalian cells

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

rsGreenF-E

Use

Tags

Expression

Mammalian

Mutation

wt

Promoter

Available since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-dCas9

Plasmid

#75381

Purpose

dCas9

Depositor

Thoru Pederson

Article

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

Sp dCas9

Use

Lentiviral

Tags

no

Expression

Mammalian

Mutation

Promoter

CMV-TO

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Cerulean(1-10)

Plasmid

#165144

Purpose

Expresses Cerulean(1-10) in mammalian cells

Depositor

Daichi Kamiyama

Article

Tamura et al Commun Biol. 2021 Feb 26;4(1):257. doi:

Insert

Cerulean(1-10)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pLV GG hUbC-dsRED

Plasmid

#84034

Purpose

Contains Golden Gate cassette to express up to four gRNAs and dsRED

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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CROPseq-multi-v2-mTagBFP2-NLS-BsmBI_entry (pRW1506)

Plasmid

#225754

Purpose

Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with nuclear-localized mTagBFP2-NLS; v2 is compatible with T7 in vitro transcription detection

Depositor

Paul Blainey

Article

Walton et al bioRxiv 2024.03.17.585235

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pT7-gRNA

Plasmid

#46759

Purpose

gRNA cloning vector for in vitro transcription of target gRNA

Depositor

Wenbiao Chen

Article

Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

Insert

gRNA core

Use

CRISPR; Zebrafish expression

Tags

Expression

Mutation

Promoter

T7

Available since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pMOS011: superecliptic pHluorin (mitochondrial)

Plasmid

#163050

Purpose

superecliptic pHluorin (mitochondrial)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

superecliptic pHluorin

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTK

Plasmid

#165841

Purpose

ganciclovir sensitivity marker

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

SR39TK

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCVII

Plasmid

#162750

Purpose

MSCV backbone

Depositor

Maki Nakayama

Article

Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCVII-tdTomato

Plasmid

#162752

Purpose

Retroviral (MSCV) expression of td Tomato fluorescent protein

Depositor

Maki Nakayama

Article

Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

Insert

tdTomato

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pmCherry

Plasmid

#165828

Purpose

mCherry red fluorescent protein CDS

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

mCherry

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pcDuex2-rsFastLime

Plasmid

#205754

Purpose

Expression of rsFastLime in mamalian cells

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

rsFastLime

Use

Tags

Expression

Mammalian

Mutation

wt

Promoter

Available since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pcDuex2-Skylan62A

Plasmid

#205755

Purpose

Expression of Skylan62A in mamalian cells

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

Skylan62A

Use

Tags

Expression

Mammalian

Mutation

wt

Promoter

Available since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pcDuex2-Dronpa

Plasmid

#205756

Purpose

Expression of Dronpa in mamalian cells

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

Dronpa

Use

Tags

Expression

Mammalian

Mutation

wt

Promoter

Available since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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CROPseq-multi-v2-SpCas9-P2A-Puro-BsmBI_entry (pRW1512)

Plasmid

#225756

Purpose

Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with expression of SpCas9 and Puromycin resistance; v2 is compatible with T7 in vitro transcription detection

Depositor

Paul Blainey

Article

Walton et al bioRxiv 2024.03.17.585235

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOS005: superecliptic pHluorin (cytosolic)

Plasmid

#163049

Purpose

superecliptic pHluorin (cytosolic)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

superecliptic pHluorin

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge