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pAS1_4xh7SKPylT_EF1_BsmBI_Intein_DD

Plasmid

#162810

Purpose

STELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient expression

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

BsmBI_Intein_DD

Use

Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*GFP

Plasmid

#162806

Purpose

STELLA plasmid for amber suppression of N-terminally labelled GFP

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

EF1_Ub-(N-TAG)GFP

Use

Piggybac

Tags

Expression

Mammalian

Mutation

GFP (Met1,Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pET-28a-CylA

Plasmid

#48198

Purpose

Expresses CylA (fatty acyl-AMP ligase) in E. coli

Depositor

Emily Balskus

Article

Nakamura et al J Am Chem Soc. 2012 Nov 14;134(45):18518

Insert

CylA

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

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mEGFP-30nmSAH-IQ

Plasmid

#61678

Purpose

GFP-labeled SAH scaffold with IQ attachment site

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

mEGFP-30nmSAH-IQ

Use

Tags

Expression

Mutation

Promoter

Available since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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pDWJ16

Plasmid

#207599

Purpose

GFP-BAA expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-BAA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pDWJ26

Plasmid

#207603

Purpose

GFP-AIA expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-AIA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pDWJ22

Plasmid

#207602

Purpose

GFP-AABA expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-AABA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pDWJ18

Plasmid

#207601

Purpose

GFP-ABA expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-ABA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pDWJ17

Plasmid

#207600

Purpose

GFP-ABB expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-ABB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pDWJ15

Plasmid

#207598

Purpose

GFP-BBA expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-BBA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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