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Plasmid#23244DepositorInsertmCerulean-2A-MYC (MYC synthetic fluorescent protein, Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf170
Plasmid#12858PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf170
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABEmax-NRTH
Plasmid#136922PurposeExpresses ABEmax-NRTH in mammalian cellsDepositorInsertABEmax-NRTH
UseCRISPRTagsExpressionMammalianMutationsee manuscriptPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST-FRT/T0-Flag-BRCA1
Plasmid#71117Purposemammalian expression of Flag-BRCA1 siRNA resistant constructDepositorInsertBRCA1 (BRCA1 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMV/TOAvailable sinceNov. 12, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET-Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS
Plasmid#89508PurposeExpression of Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS in bacterial cells.DepositorInsertrAPOBEC1-nCas9 (D10A)-UGI-NLS
UseTagsUGI and rAPOBEC-XTENExpressionBacterialMutationD10A mutation of Cas9PromoterT7Available sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRK5-FLAG-Sestrin2
Plasmid#72595PurposeoverexpressionDepositorInsertSESN2 (SESN2 Human)
UseTagsFLAGExpressionMammalianMutationcodon optimized for mammalian expression, see dep…PromoterCMVAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
NEK7_HUMAN_D0
Plasmid#79735PurposeThis plasmid encodes the kinase domain of NEK7. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertNEK7 (NEK7 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
PIK3CA-WT (pFastBac)
Plasmid#16644DepositorInsertPIK3CA (PIK3CA Human)
UseBaculoviralTagsHisExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BE4max-NRRH
Plasmid#136918PurposeExpresses BE4max-NRRH in mammalian cellsDepositorInsertBE4max-NRRH
UseCRISPRTagsExpressionMammalianMutationsee manuscriptPromoterCMVAvailable sinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BE4max-NRCH
Plasmid#136920PurposeExpresses BE4max-NRCH in mammalian cellsDepositorInsertBE4max-NRCH
UseCRISPRTagsExpressionMammalianMutationsee manuscriptPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BE4max-NRTH
Plasmid#136919PurposeExpresses BE4max-NRTH in mammalian cellsDepositorInsertBE4max-NRTH
UseCRISPRTagsExpressionMammalianMutationsee manuscriptPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMZ827
Plasmid#155321PurposeConstitutive expression of E-PIF6(1-100) in plantsDepositorInsertPCaMV35S-E-PIF6(1-100)-NLS-Tnos
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pROF394
Plasmid#155333PurposeExpression of PULSE-controlled LFY-VP16-2A-RLucDepositorInsert(etr)8-(C120)5-PhCMVmin-LFY-VP16-NLS-2A-RLuc-TSV40
UseSynthetic Biology; Eukaryotic expressionTagsExpressionMutationPromoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pROF417
Plasmid#155335PurposeConstitutive expression LFY-VP16-2A-RLuc in plantsDepositorInsertPCaMV35S-LFY-VP16-NLS-2A-RLuc-Tnos
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lifeact-7-iRFP670
Plasmid#103032PurposeExpression of iRFP670-tagged Lifeact with G418 selection in mammalian cellsDepositorInsertLifeact
UseTagsiRFP670ExpressionMammalianMutationPromoterCMVAvailable sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST-mCherry-LacR-BRCA1
Plasmid#71115Purposemammalian expression of mCherry-LacR-BRCA1 siRNA resistant constructDepositorInsertBRCA1 (BRCA1 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMV/TOAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
LENTICRISPR-TPI1_sgRNA1
Plasmid#201626PurposeCRISPR/Cas9-mediated gene knock-outDepositorInsertTPI1 (TPI1 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
LENTICRISPR-TPI1_sgRNA2
Plasmid#201627PurposeCRISPR/Cas9-mediated gene knock-outDepositorInsertTPI1 (TPI1 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
xCas9(3.6)-ABE(7.10)
Plasmid#108387PurposeMammalian expression vector for xCas9(3.6)-ABEDepositorInsertxCas9(3.6)-ABE(7.10)
UseTagsNLSExpressionMammalianMutationE108G, S217A, A262T, S409I, E480K, E543D, M694I, …PromoterAvailable sinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRK5-FLAG-Sestrin1 isoform1
Plasmid#72594Purposeoverexpression, codon optimized for mammalian expressionDepositorInsertSESN1 (SESN1 Human)
UseTagsFLAGExpressionMammalianMutationcodon optimized for mammalian expression, see dep…PromoterCMVAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only