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Plasmid#229999PurposeExpresses anti-mCherry PAGER(TF) (high reversibility) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)DepositorInsertIL2SP-Aro6-LaM8-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
UseAAVTagsExpressionMammalianMutationV360A/R361A on KORDPromoterCMVAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-anti-mCherry(LaM6) PAGER(TF)
Plasmid#229998PurposeExpresses anti-mCherry PAGER(TF) (high sensitivity) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)DepositorInsertIL2SP-Aro6-LaM6-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
UseAAVTagsExpressionMammalianMutationV360A/R361A on KORDPromoterCMVAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-anti-mCherry(LaM6) PAGER(Gq)
Plasmid#230010PurposeExpresses anti-mCherry PAGER(Gq) in mammalian cellsDepositorInsertMT1-LaM6-TEVcs-ALFA-hM1D
UseAAVTagsExpressionMammalianMutationW442A on hM1DPromoterCMVAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-DiO-eGFP-2A-mCherry_D4H
Plasmid#194880PurposeRatiometric D4H sensor for in vivo cre-dependent neuron-specific cholesterol visualizationDepositorInsertGFP-T2A-mCherry-D4H
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterhuman SynapsinAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-DiO-eGFP-2A-mCherry_YDA
Plasmid#194881PurposeRatiometric YDA sensor for in vivo cre-dependent neuron-specific cholesterol visualizationDepositorInsertGFP-T2A-mCherry-YDA
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterhuman SynapsinAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
p133Tol2-Olactb:MCS, hsp70l-mcherry-CreERT2
Plasmid#227775PurposeExpression of beta actin driven BFP with multiple cloning site at 3' end. Also contains heat-shock activated mCherry reporter and CreERT2.DepositorInsertsBFP-MCS
mCherry-t2A-CreERT2
UseZebrafish expressionTagsExpressionMutationPromoterOlactb and hsp70lAvailable sinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-U6-sgLacZ-U6-sgGFP-hSyn1-mCherry
Plasmid#208834PurposeControl plasmid expresses mCherry under the hSyn1 promoter with sgRNAs against LacZ and GFPDepositorInsertmCherry
UseAAV and CRISPRTagsExpressionBacterial and MammalianMutationPromoterhSyn1Available sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-U6-sgMap3K12(1)-U6-sgMap3K12(2)-hSyn1-mCherry
Plasmid#208835PurposeKnockout of Map3K12 (DLK) using tandem sgRNAs, expresses mCherry under the hSyn1 promoterDepositorInsertmCherry
UseAAV and CRISPRTagsExpressionBacterial and MammalianMutationPromoterhSyn1Available sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-U6-sgMap3K13(1)-U6-sgMap3K13(2)-hSyn1-mCherry
Plasmid#208836PurposeKnockout of Map3K13 (LZK) using tandem sgRNAs, expresses mCherry under the hSyn1 promoterDepositorInsertmCherry
UseAAV and CRISPRTagsExpressionBacterial and MammalianMutationPromoterhSyn1Available sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-U6-sgMap3K13(1)-U6-sgMap3K12(1)-hSyn1-mCherry
Plasmid#208837PurposeKnockout of Map3K12 (DLK) and Map3K13 (LZK) using tandem sgRNAs, expresses mCherry under the hSyn1 promoterDepositorInsertmCherry
UseAAV and CRISPRTagsExpressionBacterial and MammalianMutationPromoterhSyn1Available sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-K36M TAIL[1-44]-3AID-HA-2A-mCherryBSD
Plasmid#225872PurposeLentiviral expression of AID degron tagged H3.3(K36M) histone tail corresponding to amino acids 1-44 and mCherry fused BlasticidinRDepositorUseLentiviral and Synthetic BiologyTags3xAID HA and T2A-mCherryExpressionMammalianMutationamino acids 1-44 from H3.3 K36MPromoterAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_dCas9-XTEN-KRAB-p2A-mCherry
Plasmid#222107PurposeDonor vector for CRISPRi integration at AAVS1 with an mCherry fluorophoreDepositorInsertCRISPRi-kox1(KRAB) (cas9 S. pyogenes, Human)
UseAAV and CRISPRTagsHA and P2A-mCherryExpressionMammalianMutationD10A, H840APromoterAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a- mCherry-FIVAR-HIS-ybbR
Plasmid#169138PurposeE. coli expression of mCherry domain containing ybbR tag and FIVAR fingerprint domain. This construct was designed for AFM-SMFS measurements.DepositorInsertmCherry
UseTags6x Histag, FIVAR tag, and ybbr tagExpressionBacterialMutationPromoterAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-U6-sgPet-1-hSyn-mCherry-KASH
Plasmid#223227PurposeguideRNA targeting the mouse Pet-1 (Fev)DepositorInsertFev (Fev Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsmCherryExpressionMammalianMutationPromoterU6Available sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDRF1 StayGold-E138D-T2A-mCherry
Plasmid#219833Purposeequimolar expression of Staygold-E138D and mCherry in budding yeastDepositorInsertStayGold-E138D-T2A-mCherry
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1-PH-mCherry-MARF1 isoform D (HK56)
Plasmid#206470PurposeMARF1 expression in Schneider cells for imagingDepositorInsertMARF1 (CG17018 Fly)
UseTagsPH mcherryExpressionInsectMutationPromoterAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1-NOT1 isoform C-mCherry-PH (EB5)
Plasmid#206474PurposeNOT1 expression in Schneider cells for imagingDepositorInsertNOT1 (Not1 Fly)
UseTagsmcherry PHExpressionInsectMutationPromoterAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1-PH-mCherry-Roquin isoform A (HK63)
Plasmid#206469PurposeRoquin expression in Schneider cells for imagingDepositorInsertRoquin (roq Fly)
UseTagsPH mcherryExpressionInsectMutationPromoterAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1-PH-mCherry-Bam M24E (HK88)
Plasmid#206467PurposeBam expression in Schneider cells for imagingDepositorInsertBam (bam Fly)
UseTagsPH mcherryExpressionInsectMutationPromoterAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1-PH-mCherry-Tudor isoform A (HK130)
Plasmid#206465PurposeTudor expression in Schneider cells for imagingDepositorInsertTudor (tud Fly)
UseTagsPH mcherryExpressionInsectMutationPromoterAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only