Addgene

Showing: 2961 - 2980 of 30456 results

Results per page:

Sort results by:

pCMV5 Flag-Smad2 (1-456)

Plasmid

#14958

Depositor

Joan Massague

Article

Hata et al Nature. 1997 Jul 3. 388(6637):82-7.

Insert

Smad2 (SMAD2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Amino acids 1-456 (tail deletion)

Promoter

CMV

Available since

May 17, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV5B-Flag-Par6 S345A

Plasmid

#11749

Depositor

Jeff Wrana

Article

Ozdamar et al Science. 2005 Mar 11. 307(5715):1603-9.

Insert

PAR6 (Pard6a Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

S345A

Promoter

Available since

Aug. 4, 2006

Availability

Industry, Academic Institutions, and Nonprofits
CMV-mMaroon-KIX-mMaroon

Plasmid

#137005

Purpose

mMaroon-KIX acceptor for R-CREB

Depositor

Ryohei Yasuda

Article

Laviv et al Neuron. 2019 Dec 17. pii: S0896-6273(19)

Insert

KIX domain (Crebbp Rat)

Use

Tags

mMaroon

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-BD-cop1at(335-675)

Plasmid

#44973

Depositor

Thanos Halazonetis

Article

Crefcoeur et al Nat Commun. 2013;4:1779. doi: 10.1038/nc

Insert

BD-COP1 (COP1 Mustard Weed)

Use

Tags

GAL4 DNA-binding domain

Expression

Mammalian

Mutation

deleted amino acids 1-334

Promoter

pCMV

Available since

June 5, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-MTS-FLAG-LOV*

Plasmid

#202207

Purpose

Expresses the photoproximity labeling catalyst LOV* fused to the mitochondria targeting sequence (MTS) in mammalian cells

Depositor

Thomas Muir

Article

Hananya et al Proc Natl Acad Sci U S A. 2023 Apr 18;12

Insert

Cytochrome c oxidase subunit 4 isoform 1, mitochondrial, aa 1-24

Use

Tags

FLAG and LOV*

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLA CMV N-Flag RALB WT

Plasmid

#50990

Purpose

Expresses RALB in mammalian cells

Depositor

Anna Sablina

Article

Simicek et al Nat Cell Biol. 2013 Oct;15(10):1220-30.

Insert

RALB (RALB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 19, 2014

Availability

Academic Institutions and Nonprofits only
pMuLE ENTR CMV LacZ L5-L2

Plasmid

#62166

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and LacZ module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

LacZ

Use

Mule gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-VHL R167W-pRc/CMV

Plasmid

#22082

Depositor

William Kaelin

Article

Kibel et al Science. 1995 Sep 8. 269(5229):1444-6.

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Use

Tags

HA-tag

Expression

Mammalian

Mutation

point mutant converting aa 167 from Arginine to T…

Promoter

Available since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV-A3A-seBE4max-IRES

Plasmid

#174698

Purpose

Small-molecule controlled base editing

Depositor

Rahul Kohli

Article

Berrios et al Nat Chem Biol. 2021 Dec;17(12):1262-1270

Insert

A3A-seBE4max-IRES (APOBEC3A Human)

Use

Tags

myc tag

Expression

Mammalian

Mutation

A3A-M13I, UGI-E11K

Promoter

CMV

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-myc-ASPM CTR

Plasmid

#27669

Depositor

Joseph Loturco

Article

Paramasivam et al Cell Cycle. 2007 Jul 1. 6(13):1605-12.

Insert

Abnormal spindle-like microcephaly associated (Aspm Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

Contains carboxy terminal region of ASPM (amino a…

Promoter

Available since

March 21, 2011

Availability

Academic Institutions and Nonprofits only
pPW3763 : CMVd3-HsIRE1dLKR-HaloTag

Plasmid

#185685

Purpose

Low-level transient expression of HsIRE1 lacking the linker, kinase, and RNase domains, with a C-terminal HaloTag.

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERN1 (ERN1 Human, Synthetic)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Deletion of linker, kinase, and Rnase domains

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV Lex VP16 HA (P#1708)

Plasmid

#14593

Depositor

Matija Peterlin

Article

Kurosu et al Curr Biol. 2004 Jun 22. 14(12):1112-6.

Insert

LexA

Use

Tags

HA and VP16

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
pCMV5 Flag-Smad3 (1-145)

Plasmid

#14965

Depositor

Joan Massague

Article

Chen et al Cell. 2002 Jul 12. 110(1):19-32.

Insert

Smad3 (SMAD3 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

aa 1-145

Promoter

Available since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
pCMV-SPORT6-RNF114-UIMmut

Plasmid

#69923

Purpose

Expresses mutant myc-RNF114 in mammalian cells

Depositor

Francesca Capon

Article

Bijlmakers et al Hum Mol Genet. 2011 Aug 15;20(16):3129-3

Insert

RNF114 (RNF114 Human)

Use

Tags

Expression

Mammalian

Mutation

S224P

Promoter

CMV

Available since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only
pCMV 3xFLAG-GABARAPL1 G116A

Plasmid

#123101

Purpose

Expresses 3xFLAG-GABARAPL1 G116A in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Glycine 116 to Alanine

Promoter

CMV

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV mTurquoise2-superYFP tandem

Plasmid

#157776

Purpose

Produces a fusion between mTurquoise2 and superYFP. It can be used as a positive control for FRET (using FLIM)

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Type

Empty backbone

Use

Tags

mTurquoise2 and superYFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV6 Myc PAK5 K478M

Plasmid

#16020

Depositor

Jonathan Chernoff

Article

Cotteret et al Mol Cell Biol. 2003 Aug . 23(16):5526-39

Insert

PAK5 (PAK5 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

K478M: kinase dead

Promoter

Available since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only
pCMV Myc rat SAPAP3

Plasmid

#40217

Depositor

Yutaka Hata, Yoshimi Takai

Article

Takeuchi et al J Biol Chem. 1997 May 2;272(18):11943-51

Insert

SAPAP3 (Dlgap3 Rat)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
pX601‐mhCMV‐ABEmaxNGA‐C3‐ E53ogRNA

Plasmid

#187063

Purpose

Npu Split C-terminal half of ABEmaxNGA with gRNA targeting mdx4cv nonsense mutation

Depositor

Renzhi Han

Article

Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

Insert

Npu Split C-terminal half of ABEmaxNGA with gRNA targeting mdx4cv nonsense mutation

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

mhCMV

Available since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
IG301: pMVP (L1-R5) CMV promoter

Plasmid

#121692

Purpose

pMVP L1-R5 entry plasmid, contains CMV promoter for 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

CMV promoter

Use

Synthetic Biology; Pmvp gateway entry plasmid

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge