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Addgene

pCMV6 Myc PAK5 K478M
(Plasmid #16020)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16020 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCMV6-myc
  • Backbone size w/o insert (bp) 4900
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PAK5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2200
  • Mutation
    K478M: kinase dead
  • Entrez Gene
    PAK5 (a.k.a. PAK7)
  • Tag / Fusion Protein
    • Myc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI/BglII (destroyed during cloning)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Order of elements: SalI-Myc-XbaI-Pak5-EcoRI

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV6 Myc PAK5 K478M was a gift from Jonathan Chernoff (Addgene plasmid # 16020 ; http://n2t.net/addgene:16020 ; RRID:Addgene_16020)
  • For your References section:

    p21-Activated kinase 5 (Pak5) localizes to mitochondria and inhibits apoptosis by phosphorylating BAD. Cotteret S, Jaffer ZM, Beeser A, Chernoff J. Mol Cell Biol. 2003 Aug . 23(16):5526-39. 10.1128/MCB.23.16.5526-5539.2003 PubMed 12897128