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Showing: 5921 - 5960 of 148587 results

  1. Banshee shTCF1 mCherry

    Plasmid
    #80154
    Purpose
    expresses shRNA targeting TCF1 and mCherry
    Depositor
    Ellen Rothenberg
    Insert
    TCF1
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    80154-plasmid-map-sequence-id-158534

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  2. pICE-HA-MRE11-H63D

    Plasmid
    #82036
    Purpose
    Plasmid for constitutive or doxycycline-inducible expression of H63D exonuclease-dead mutant of human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.
    Depositor
    Sébastien Britton, Patrick Calsou
    Article
    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10
    Insert
    MRE11 (MRE11 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Exonuclease-dead mutant: H63D MRE11
    Promoter
    CMV-tet
    Available since
    Sept. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82036-plasmid-map-sequence-id-155389

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  3. pT076

    Plasmid
    #137880
    Purpose
    Donor vector for integration at the human AAVS1 safe harbor locus of Doxicycline-inducible dCas9-VPR-T2A-EGFP
    Depositor
    Lindy Barrett
    Article
    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.
    Insert
    TRE3G-dCas9-VPR-T2A-EGFP-CAG-TetON-NeoR-SA
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    137880-plasmid-map-sequence-id-271983

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  4. CMV-R-GECO1

    Plasmid
    #32444
    Purpose
    Mammalian expression of Red fluorescent genetically encoded Ca2+ indicator for optical imaging
    Depositor
    Robert Campbell
    Article
    Zhao et al Science. 2011 Sep 8.
    Insert
    R-GECO1.0
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Substitutions relative to the mApple-derived anal…
    Promoter
    CMV
    Available since
    Sept. 29, 2011
    Availability
    Academic Institutions and Nonprofits only
    32444-plasmid-map-sequence-id-117093

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  8. HaloTag-A2A-adenosine receptor

    Plasmid
    #66995
    Purpose
    A2A-adenosine receptor tagged with HaloTag on amino terminus
    Depositor
    Catherine Berlot
    Insert
    HaloTag-A2A-adenosine receptor (ADORA2A Human)
    Use
    Tags
    Human Growth hormone Signal Sequence- HaloTag
    Expression
    Mammalian
    Mutation
    The signal sequence from human growth hormone was…
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    66995-plasmid-map-sequence-id-115422

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  17. SIRT6

    Plasmid
    #41564
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SIRT6 (SIRT6 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    aa 3-318
    Promoter
    T7
    Available since
    April 9, 2013
    Availability
    Academic Institutions and Nonprofits only

  23. pICE-HA-MRE11-H63S

    Plasmid
    #82035
    Purpose
    Plasmid for constitutive or doxycycline-inducible expression of H63S mutant of human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.
    Depositor
    Sébastien Britton, Patrick Calsou
    Article
    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10
    Insert
    MRE11 (MRE11 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    H63S MRE11 mutant
    Promoter
    CMV-tet
    Available since
    Sept. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82035-plasmid-map-sequence-id-155388

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  27. pLV_Hygro_mini-mAgrin-Myc-His

    Plasmid
    #198130
    Purpose
    Express mini-Agrin in mammalian cells
    Depositor
    Jeffrey Miner
    Insert
    mini-Agrin (Agrn Mouse)
    Use
    Lentiviral
    Tags
    TEV-Myc-His
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    June 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    198130-plasmid-map-sequence-id-395039

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  28. AAV-EF1a-DIO-ChIEF(E162A/T198C)-P2A-dTomato-WPRE-BGHpA

    Plasmid
    #51095
    Purpose
    Cre dependent expression of ChIEF kinetic variant E162A/T198C with physically uncoupled dTomato fluorophore
    Depositor
    Jonathan Ting
    Insert
    ChIEF(E162A/T198C)
    Use
    AAV
    Tags
    P2A-dTomato
    Expression
    Mammalian
    Mutation
    E162A/T198C
    Promoter
    EF1a
    Available since
    May 13, 2014
    Availability
    Academic Institutions and Nonprofits only
    51095-plasmid-map-sequence-id-79317

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  34. BPK764

    Plasmid
    #65767
    Purpose
    Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Bacterial
    Mutation
    Promoter
    T7 (x2)
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65767-plasmid-map-sequence-id-112648

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  38. pMT1093 human L1 EN- (ORF2p E43S, D145N) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid
    #213028
    Purpose
    Expresses endonuclease dead (EN- ORF2p E43S, D145N) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (ORFeus-Hs codon optimized sequence, EN- E43S D145N) with ORF2-3xFlag
    Use
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Mutation
    E43S D145N double mutant EN- endonuclease catalyt…
    Promoter
    CMV
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    213028-plasmid-map-sequence-id-422046

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Showing: 5921 - 5960 of 148587 results