Skip to main content
Addgene
Showing: 2961 - 2980 of 148440 results

  5. pcDNA3/mStayGold(c4)=UtrCH

    Plasmid
    #212020
    Purpose
    For filamentous actin labeling. Alternatively, the UtrCH gene can be replaced with a target gene for N-terminal tagging with mStayGold via a c4 adaptor and a linker [(GGGGS)3] (denoted as ‘=’).
    Depositor
    Atsushi Miyawaki
    Article
    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s
    Insert
    UtrCH
    Use
    Tags
    mStayGold(c4)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    212020-plasmid-map-sequence-id-419202

    Enlarge

  7. MBP-Benzonase

    Plasmid
    #218192
    Purpose
    Bacterial expression of a periplasmically expressed MBP-tagged Serratia Marcescens NucA (Benzonase); also known as c001
    Depositor
    Cheryl Arrowsmith
    Insert
    nucA
    Use
    Tags
    MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    218192-plasmid-map-sequence-id-431003

    Enlarge

  10. Fragment1 with TLS2

    Plasmid
    #196983
    Purpose
    Gateway-compatible gRNA backbone with TLS2 mobility signal. Template for the addition of target sequences to produce 2x graft-mobile gRNA-TLS2.
    Depositor
    Friedrich Kragler
    Article
    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    Intermediate fragment for assembly of gRNA-TLS2 construct
    Use
    Gateway-compatible entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196983-plasmid-map-sequence-id-390457

    Enlarge

  13. pNF02-mLemon

    Plasmid
    #219399
    Purpose
    Constitutive expression of mLemonin E. coli
    Depositor
    Christian Lesterlin
    Insert
    mLemon
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    proDp
    Available since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    219399-plasmid-map-sequence-id-436555

    Enlarge

  14. pNF02-mChartreuse

    Plasmid
    #219397
    Purpose
    Constitutive expression of mChartreuse in E. coli
    Depositor
    Christian Lesterlin
    Insert
    mChartreuse
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    proDp
    Available since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    219397-plasmid-map-sequence-id-436559

    Enlarge

  15. MSCV-IRES-GFP

    Plasmid
    #20672
    Purpose
    Depositor
    Tannishtha Reya
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    IRES EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 28, 2009
    Availability
    Academic Institutions and Nonprofits only
    20672-plasmid-map-sequence-id-203241

    Enlarge

  18. pCAT005

    Plasmid
    #171632
    Purpose
    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting B2M and an EF1-a promoter expressing mNeonGreen
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-B2M
    mNeon
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1-a and U6
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171632-plasmid-map-sequence-id-336487

    Enlarge

  20. Inducible Caspex expression

    Plasmid
    #97421
    Purpose
    For stable clone generation of cells expressing the dCas9-APEX2 fusion protein for Genomic locus proteomics
    Depositor
    Samuel Myers, Steven Carr
    Article
    Myers et al Nat Methods. 2018 May 7. pii: 10.1038/s4
    Inserts
    Caspex
    T2A-Gfp
    Use
    CRISPR
    Tags
    3xFLAG and V5
    Expression
    Mammalian
    Mutation
    dCas9: D10A and H840A, APEX2: K14D, W41F, E112K, …
    Promoter
    TRE3G
    Available since
    Oct. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    97421-plasmid-map-sequence-id-235343

    Enlarge

Showing: 2961 - 2980 of 148440 results