Addgene

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pMS2-GFP

Plasmid

#27121

Depositor

Robert Singer

Article

Fusco et al Curr Biol. 2003 Jan 21. 13(2):161-7.

Insert

MS2

Use

Tags

EGFP, HA, and SV40 NLS

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF

Plasmid

#116719

Purpose

Lentiviral expression of BRAF

Depositor

Kenneth Scott, Gordon Mills

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Tags

Expression

Mutation

WT

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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mPlum-pBAD

Plasmid

#54564

Purpose

Localization: Protein Expression Vector , Excitation: 590, Emission: 649

Depositor

Roger Tsien, Michael Davidson

Article

Wang et al Proc Natl Acad Sci U S A. 2004 Nov 30;10

Type

Empty backbone

Use

Tags

6xHis and mPlum

Expression

Bacterial

Mutation

Promoter

Available since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

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TOM20MTS-mCherry-EGFP-Tet-On

Plasmid

#109016

Purpose

Tet-On construct to induce expression of tandem eGFP-mCherry tagged TOM20MTS (mitochondria

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

TOM20MTS (MTS: mitochondria targeting signal) (TOMM20 Human)

Use

Lentiviral

Tags

mCherry-eGFP

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pFR_CrPV_xb

Plasmid

#11509

Depositor

Phil Sharp

Article

Petersen et al Mol Cell. 2006 Feb 17. 21(4):533-42.

Insert

Firefly luciferase, Cricket Paralysis Virus IRES sequence, Renilla, and 3'UTR short RNA binding sites

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

March 15, 2006

Availability

Academic Institutions and Nonprofits only

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pMKO.1 puro

Plasmid

#8452

Depositor

Bob Weinberg

Article

Stewart et al RNA 2003 Apr;9(4):493-501.

Type

Empty backbone

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2005

Availability

Academic Institutions and Nonprofits only

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pET His6 Sumo TEV LIC cloning vector (2S-T)

Plasmid

#29711

Depositor

Scott Gradia

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Tags

His6-Sumo-TEV

Expression

Bacterial

Mutation

Promoter

Available since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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pTXB1-nbMmIgG1-Tn5

Plasmid

#184287

Purpose

Anti-mouse IgG1 Fc specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)

Depositor

Ivan Raimondi, New York Genome Center

Article

Stuart et al Nat Biotechnol. 2022 Dec 19. doi: 10.103

Insert

nbMmIgG1-Tn5

Use

Tags

Expression

Mutation

Promoter

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pAVU6+27-F30-2xdBroccoli

Plasmid

#66842

Purpose

Expresses two dimeric Broccoli aptamers in the F30 scaffold in mammalian cells

Depositor

Samie Jaffrey

Article

Filonov et al Chem Biol. 2015 May 21;22(5):649-60. doi

Insert

Two dimeric Broccoli aptamers in the F30 scaffold

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 25, 2015

Availability

Academic Institutions and Nonprofits only

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pCDF-GFPplus

Plasmid

#172718

Purpose

Expression of a fast folding GFP variant GFP plus

Depositor

Tilmann Weber

Article

Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

Insert

GFP plus

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA PTEN

Plasmid

#78776

Purpose

To overexpress PTEN in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al Nat Commun. 2015 Jul 17;6:7769. doi: 10.

Insert

PTEN (PTEN Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223-ERBB3

Plasmid

#23874

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ERBB3 (ERBB3 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/V5-His-TOPO-mir17-92

Plasmid

#21109

Depositor

Joshua Mendell

Article

O'Donnell et al Nature. 2005 Jun 9. 435(7043):839-43.

Insert

miR-17-92 cluster (MIR17 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA

Plasmid

#20297

Purpose

Cre-activated AAV expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activation

Depositor

Karl Deisseroth

Has Service

AAV Retrograde, AAV1, AAV5, AAV8, and AAV9

Insert

channelrhodopsin-2

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

humanized ChR2 gene with histidine 134 changed to…

Promoter

EF1alpha

Available since

June 23, 2009

Availability

Academic Institutions and Nonprofits only

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pDMyc-TNPO1

Plasmid

#86081

Purpose

A mammalian expression plasmid encoding Transportin-1 (TNPO1) with N-terminus double Myc-tag.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Transportin-1 (TNPO1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

T7

Available since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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amilCP chromoprotein

Plasmid

#117847

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses amilCP chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, amilCP

Use

Synthetic Biology; Escherichia coli

Tags

Expression

Mutation

Promoter

Available since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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3XFlagNICD2

Plasmid

#20184

Depositor

Raphael Kopan

Article

Ong et al J Biol Chem. 2006 Feb 24. 281(8):5106-19

Insert

Notch 2 intracellular domain (Notch2 Mouse)

Use

Tags

3X FLAG

Expression

Mammalian

Mutation

Contains murine Notch 2 Intracellular Domain (Met…

Promoter

CMV

Available since

Jan. 15, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn1-SIO-stGtACR2-FusionRed

Plasmid

#105677

Purpose

Expresses a soma-targeted GtACR2 in Cre-expressing neurons

Depositor

Ofer Yizhar

Article

Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1

Has Service

AAV Retrograde, AAV1, and AAV5

Insert

stGtACR2

Use

AAV

Tags

FusionRed and Kv2.1

Expression

Mammalian

Mutation

Promoter

hSyn1

Available since

Feb. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pmTurquoise2-Golgi

Plasmid

#36205

Purpose

In vivo visualization of golgi apparatus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

Golgi targeting sequence (B4GALT1 Human)

Use

Tags

mTurquoise2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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GFP-ASH1L

Plasmid

#65369

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

ASH1L (ASH1L Human)

Use

Tags

EmGFP

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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