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Showing: 2941 - 2960 of 3923 results

  1. PX459v3-eSpCas9(1.1)

    Plasmid
    #178800
    Purpose
    High-fidelity eSpCas9(1.1) with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).
    Depositor
    Paul Thomas
    Article
    Chey et al bioRxiv 2024.07.19.604224
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Cbh (Cas9-2A Puro) and U6 (gRNA)
    Available since
    Aug. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    178800-plasmid-map-sequence-id-354839

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  2. PX459v3-SpCas9-HF1

    Plasmid
    #178801
    Purpose
    High-fidelity SpCas9-HF1 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).
    Depositor
    Paul Thomas
    Article
    Chey et al bioRxiv 2024.07.19.604224
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Cbh (Cas9-2A Puro) and U6 (gRNA)
    Available since
    Aug. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    178801-plasmid-map-sequence-id-354837

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  11. MBP-ATG13 (450-517)-ULK1 (836-1050)-His

    Plasmid
    #222825
    Purpose
    Bacteria expression of MBP-ATG13 (450-517) and ULK1 MIT domain fusion complex -6His
    Depositor
    James Hurley
    Insert
    ATG13, ULK1 (ATG13, ULK1 Human)
    Use
    Tags
    6His and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    222825-plasmid-map-sequence-id-438334

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  12. MBP-ATG13 (363-517)-ULK1 (836-1050)-His

    Plasmid
    #222824
    Purpose
    Bacteria expression of MBP-ATG13 C-terminal and ULK1 MIT domain fusion complex -6His
    Depositor
    James Hurley
    Insert
    ATG13, ULK1 (ATG13, ULK1 Human)
    Use
    Tags
    6His and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    222824-plasmid-map-sequence-id-438332

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  13. His-GST-ATG13 (363-517)-ULK1 (836-1050)

    Plasmid
    #222822
    Purpose
    Bacteria expression of His-GST -ATG13 C-terminal and ULK1 MIT domain fusion complex
    Depositor
    James Hurley
    Insert
    ATG13, ULK1 (ATG13, ULK1 Human)
    Use
    Tags
    6His-GST
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    222822-plasmid-map-sequence-id-438327

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  14. pCAG-TSF-ATG13 (363-517)-ULK1 (836-1050)-msfGFP

    Plasmid
    #222821
    Purpose
    Mammalian expression of TwinStrep-Flag -ATG13 C-terminal and ULK1 MIT domain fusion complex fused with GFP-10His
    Depositor
    James Hurley
    Insert
    ATG13, ULK1 (ATG13, ULK1 Human)
    Use
    Tags
    TwinStrep Flag and msfGFP-10His
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    222821-plasmid-map-sequence-id-438323

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  15. lenti-EGFR-L858R-T790M-dual-nick sgRNA

    Plasmid
    #214101
    Purpose
    Lentiviral vector expressing nicking sgRNAs for the induction of EGFR L858R and T790M mutations, through tandem U6 expression of the two sgRNAs using independent U6 promoters.
    Depositor
    Ting Zhou
    Article
    Wu et al bioRxiv [Preprint]. 2024 Jan 19:2024.01.
    Insert
    EGFR L858R nicking sgRNA/EGFR T790M nicking sgRNA
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6
    Available since
    June 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    214101-plasmid-map-sequence-id-429357

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  19. pILGFPEasy9R

    Plasmid
    #218291
    Purpose
    Plasmid-free in-situ promoter cloning and characterisation system in S. cerevisiae, allows the characterisation of a bidirectional promoter using yEGFP and E2-Crimson as reporters.
    Depositor
    Claudia Vickers
    Article
    McDonnell et al bioRxiv 2024.03.26.586389
    Insert
    pURA3>KlURA3>tKlURA3-tPGK1KanMX>tAgTEF-pMAL32>MazF(RPL28i)-E2Crimson>tPDC1-pDDI2>ISceI>tSynth3>ura3(4,191)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218291-plasmid-map-sequence-id-433736

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  20. pToxAmp21R

    Plasmid
    #218287
    Purpose
    A complete plasmid for ToxAmp (Toxin-antitoxin-driven gene amplification) for the expression of AeBlue
    Depositor
    Claudia Vickers
    Article
    Evans et al bioRxiv 2024.01.30.578080
    Insert
    HO(-955, -789)-LoxP>pKlLEU2>KlLEU2>PCRT1>RelB>tPDC1-pTDH3>AeBlue>tSYNth7-ARS712-HO(-731, -264)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218287-plasmid-map-sequence-id-433689

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Showing: 2941 - 2960 of 3923 results