Addgene

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PKC delta DN

Plasmid

#16389

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC delta isoform 2 (Prkcd Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Dominant negative (kinase inactive): K376R

Promoter

Available since

April 28, 2009

Availability

Academic Institutions and Nonprofits only

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pHAGE PGK-GFP-IRES-LUC-W

Plasmid

#46793

Purpose

2nd generation lentiviral vector

Depositor

Darrell Kotton

Article

Wilson et al J Clin Invest. 2010 Jan . 120(1):379-89.

Inserts

PGK
eGFP (eGFP )
IRES
Luciferase

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pRSETA-PAGFP

Plasmid

#11911

Depositor

Jennifer Lippincott-Schwartz

Article

Patterson et al Science. 2002 Sep 13. 297(5588):1873-7.

Insert

pRSETA-PAGFP

Use

Tags

Expression

Bacterial

Mutation

The PAGFP has four mutations (L64F/T65S/V163A/T20…

Promoter

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

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pET30a-AlkB-D135S

Plasmid

#79051

Purpose

Express mutant AlkB protein (D135S) in E. coli BL21(DE3)

Depositor

Tao Pan

Article

Zheng et al Nat Methods. 2015 Sep;12(9):835-7. doi:

Insert

AlkB (alkB E.coli)

Use

Tags

His tag

Expression

Bacterial

Mutation

A truncated AlkB with deletion of the amino (N)-t…

Promoter

T7

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-SARS-CoV-2-nsp8-2xStrep-IRES-Puro

Plasmid

#141374

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp8 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBabe 12S E1A

Plasmid

#18742

Depositor

Scott Lowe

Article

Samuelson et al Proc Natl Acad Sci U S A. 1997 Oct 28. 9

Insert

12S E1A

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

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CbS-Flag-TRRAP

Plasmid

#32103

Depositor

Yardena Samuels, Michael Cole

Insert

Transformation/transcription domain-associated protein (TRRAP Human)

Use

Tags

Flag 1x

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pLH-spsgRNA2

Plasmid

#64114

Purpose

Vector for expression of Sp sgRNA2 in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Sp sgRNA2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO.DEST.EGFP

Plasmid

#32684

Purpose

Lentiviral cDNA/shRNA gateway destination vectors

Depositor

Ming-Sound Tsao

Article

Radulovich et al BMC Biotechnol. 2011 Mar 22;11:24.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 1, 2014

Availability

Academic Institutions and Nonprofits only

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pRRL SFFV d20GFP.T2A.mTagBFP Donor

Plasmid

#31485

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

SFFV d20BFP T2A BFP

Use

Lentiviral

Tags

Expression

Mutation

GFP has 20 amino acids deleted from the C terminu…

Promoter

Available since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pJFRC81-10XUAS-IVS-Syn21-GFP-p10

Plasmid

#36432

Depositor

Gerald Rubin

Article

Pfeiffer et al Proc Natl Acad Sci U S A. 2012 Apr 9.

Insert

GFP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

May 3, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV CD68-hM4D(Gi)-mCherry

Plasmid

#75033

Purpose

Expression of hM4D(Gi) using the CD68 promoter.

Depositor

Bryan Roth

Article

Grace et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Has Service

AAV1 and AAV9

Insert

hM4D(Gi)-mCherry

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CD68

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pMSCV-FlagMLL-pl-ENL(5613)

Plasmid

#20873

Depositor

Robert Slany

Article

Bach et al Oncogene. 2009 Feb 12. 28(6):815-23.

Insert

MLL (KMT2A, MLLT1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

April 21, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA-HDAC6-FLAG

Plasmid

#30482

Depositor

Tso-Pang Yao

Article

Kawaguchi et al Cell. 2003 Dec 12. 115(6):727-38.

Insert

HDAC6 (HDAC6 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pBABE-hygro p53 DD

Plasmid

#9058

Depositor

Bob Weinberg

Article

Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Insert

p53 DD (Trp53 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

DD. Dominant negative mutant carries deletion of…

Promoter

Available since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

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Flag-Bcl2

Plasmid

#18003

Depositor

Clark Distelhorst

Article

Wang et al J Biol Chem. 2001 Nov 23. 276(47):44117-

Insert

Bcl-2 (BCL2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

June 4, 2008

Availability

Academic Institutions and Nonprofits only

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pLVPT-tTR-KRAB

Plasmid

#11642

Purpose

Tet-regulated (Tet-on) lentiviral vector for transgene (hPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

Depositor

Didier Trono, Patrick Aebischer

Article

Szulc et al Nat Methods. 2006 Feb . 3(2):109-16.

Insert

mPGK, GFP, tTR-KRAB, Tet-on

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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pRK5-HA GST RagC 75L

Plasmid

#19305

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding C (RRAGC Human)

Use

Tags

GST and HA

Expression

Mammalian

Mutation

S75L

Promoter

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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mOrange2-N1

Plasmid

#54568

Purpose

Localization: N1 Cloning Vector, Excitation: 549, Emission: 565

Depositor

Nathan Shaner, Roger Tsien, Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Type

Empty backbone

Use

Tags

mOrange2

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_pMagFast2(3x)-iRFP

Plasmid

#67297

Purpose

Blue light dependent photoswitching system

Depositor

Moritoshi Sato

Article

Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

Insert

pMagFast2(3x)-iRFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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