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Plasmid#10943DepositorInsertPTEN (PTEN Human)
UseRetroviralTagsExpressionMammalianMutation360-364A (SSSTS to AAAAA); A4: S380A, T382A, T383…PromoterAvailable sinceJan. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
integrin alpha9 / alpha5 EGFP pBabe puro
Plasmid#13608DepositorInsertintegrin alpha 9 / alpha 5 (ITGA9 Human)
UseRetroviralTagsGFPExpressionMammalianMutationIntegrin alpha 9 chimera containing the cytoplasm…PromoterAvailable sinceJan. 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLenti-VRERRA-P2A-GFP-PGK-Puro
Plasmid#110865PurposeLentiviral vector for constitutive expression of Cas9-VRERRA-P2A-GFP in mammalian cells (codon optimized)DepositorInsertCas9-VRERRA
UseLentiviralTagsFLAGExpressionMutationD1135V, G1218R, R1335E, T1337R, and NLS sequence …PromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSCV-6NBC-GFP-PGK-Puro-Barcode83
Plasmid#83760Purposeindividual 6N barcode to make barcoded cell line variantsDepositorInsertBarcode83
UseRetroviralTagsExpressionMutationPromoterAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
N112 nLV EF-1a-PGK-Puro Gal4-HP1a CS
Plasmid#43969DepositorInsertCbx5 (Cbx5 Mouse)
UseLentiviralTagsGal4/DBDExpressionMutationN-term chromo domain removedPromoterEF-1aAvailable sinceJune 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
1038 pBabe puroL PTEN 360-364A
Plasmid#10940DepositorInsertPTEN (PTEN Human)
UseRetroviralTagsExpressionMammalianMutation360-364A (SSSTS to AAAAA)PromoterAvailable sinceJan. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
hPlekhm1-C/pIRES puro Glue (H987-end)
Plasmid#73458PurposeExpress human C-terminal part (H987-end) Plekhm1 in mammalian cellsDepositorInsertPlekhm1 (PLEKHM1 Human)
UseTagsCBP tag, HA tag, and strep tagExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
hPlekhm1-C/pIRES puro Glue (S778-Q986)
Plasmid#73457PurposeExpress human C-terminal part (S778-Q986) Plekhm1 in mammalian cellsDepositorInsertPlekhm1 (PLEKHM1 Human)
UseTagsCBP tag, HA tag, and strep tagExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAV-puro-U6+27-F30-Tornado-Acyclovir-IRE#A4
Plasmid#216823PurposeExpresses acyclovir-controllable IRE variant #A4 as circular RNA in mammalian cells using the Tornado expression system.DepositorInsertTornado-Acyclovir-IRE#A4
UseTagsExpressionMammalianMutationPromoterU6+27Available sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV-puro-U6+27-F30-Tornado-Acyclovir-IRE#A8
Plasmid#216824PurposeExpresses acyclovir-controllable IRE variant #A8 as circular RNA in mammalian cells using the Tornado expression system.DepositorInsertTornado-Acyclovir-IRE#A8
UseTagsExpressionMammalianMutationPromoterU6+27Available sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV-puro-U6+27-F30-Tornado-Acyclovir-IRE#A8-3
Plasmid#216825PurposeExpresses acyclovir-controllable IRE variant #A8-3 as circular RNA in mammalian cells using the Tornado expression system.DepositorInsertTornado-Acyclovir-IRE#A8-3
UseTagsExpressionMammalianMutationPromoterU6+27Available sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV-puro-U6+27-F30-Tornado-Acyclovir-IRE#A8-4
Plasmid#216826PurposeExpresses acyclovir-controllable IRE variant #A8-4 as circular RNA in mammalian cells using the Tornado expression system.DepositorInsertTornado-Acyclovir-IRE#A8-4
UseTagsExpressionMammalianMutationPromoterU6+27Available sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCW-HA-hRb-WT-T373A-S608A-S612A-puro
Plasmid#212674PurposeExpress tagged hRb mutantDepositorInsertRb (RB1 Human)
UseLentiviralTagsHAExpressionMutationT373A/S608A/S612APromoterTREAvailable sinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro cTnT CCND2-T2A-ChloC
Plasmid#179841Purposedonor plasmid for cardiac-specific CCND2-T2A-Chloc expression in human cellsDepositorInsertCCND2 (CCND2 Human)
UseCRISPR and TALEN; Donor plasmidTagschloride-conducting ChR (ChloC)ExpressionMammalianMutationPromoterTroponin T promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro cTnT CCND2-T2A-iChloC
Plasmid#179842Purposedonor plasmid for cardiac-specific CCND2-T2A-iChloc expression in human cellsDepositorInsertCCND2 (CCND2 Human)
UseCRISPR and TALEN; Donor plasmidTagsimproved chloride-conducting ChR (iChloC)ExpressionMammalianMutationPromoterTroponin T promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro cTnT CCND2-T2A-luciferase
Plasmid#179844Purposedonor plasmid for cardiac-specific CCND2-T2A-luciferase expression in human cellsDepositorInsertCCND2 (CCND2 Human)
UseCRISPR, Luciferase, and TALEN ; Donor plasmidTagsluciferaseExpressionMammalianMutationPromoterTroponin T promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-EF1A-Puro-mCherry-RAB7-S72A
Plasmid#221556PurposeExpresses Cherry-tagged human Rab7 with S72A mutation in mammalian cells. Compatible with piggybac transposase for genome integration.DepositorInsertRab7A (RAB7A Human)
UsePiggybacTagsmCherryExpressionMammalianMutationChange serine 72 to alaninePromoterEF1aAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-EF1A-Puro-hTBK1-E696K-EGFP
Plasmid#221554PurposeExpresses GFP-tagged human TBK1 with E696K mutation in mammalian cells. Compatible with piggybac transposase for genome integration.DepositorInsertTBK1 (TBK1 Human)
UsePiggybacTagsGFPExpressionMammalianMutationChanged glutamate 696 to lysinePromoterEF1aAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
p944-SWITCH-OVER insert: EF1a-Puro
Plasmid#217891Purposeinsert vector for Switch-OVER: Single loxP-STOP-EF1a-Puro-mU6DepositorTypeEmpty backboneUseCRISPR, Cre/Lox, and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKL038 Lenti U6 dCas12a gRNA Puro mCherry
Plasmid#195546PurposedCas12a gRNA expression backboneDepositorArticleInsertLenti U6- empty cassette_Direct repeat_puro_mcherry
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only