Addgene

We narrowed to 4,919 results for: pcDNA3.1+

Showing: 2921 - 2940 of 4919 results

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pcDNA-Mirk (YF)

Plasmid

#17845

Depositor

Eileen Friedman

Article

Lee et al Cancer Res. 2000 Jul 1. 60(13):3631-7.

Insert

Mirk (DYRK1B Human)

Use

Tags

6xHis and Anti-XPress

Expression

Mammalian

Mutation

Y271/273F

Promoter

Available since

April 29, 2008

Availability

Academic Institutions and Nonprofits only
HA-RNF126 C231A

Plasmid

#138648

Purpose

Mammalian expression of full-length2 HA-tagged RNF126 with an inactivating mutation in the RING domain (C231A)

Depositor

Jane McGlade

Article

Smith et al Exp Cell Res. 2014 Jan 15;320(2):219-32.

Insert

RNF126 (RNF126 Mouse)

Use

Tags

HA-HA

Expression

Mammalian

Mutation

C231A

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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YFP Gamma 9

Plasmid

#36107

Depositor

Narasimhan Gautam

Article

Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

Insert

G protein subunit Gamma 9

Use

Tags

YFP

Expression

Mammalian

Mutation

HIs tag between YFP and insert

Promoter

CMV

Available since

May 3, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA-ELYS-emerin-EGFP-polyA

Plasmid

#59745

Purpose

Express ELYS-emerin-EGFP fusion protein in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

ELYS-emerin-EGFP

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pFOX6-Tier1-PhCMV-TdTomato

Plasmid

#169515

Purpose

Tier-1 vector encoding PhCMV-driven bright-red fluorescent protein TdTomato (PhCMV-TdTomato-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven dimeric red fluorescent protein TdTomato

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

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pmyc-GFP-TNRC6A-NES-mut

Plasmid

#42001

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Use

Tags

EGFP and Myc

Expression

Mammalian

Mutation

F951A, F955A, I958A, F960A

Promoter

CMV

Available since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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optGluCl beta Y182F ECFP

Plasmid

#15105

Depositor

Henry Lester

Article

Slimko et al J Neurosci Methods. 2003 Mar 30. 124(1):

Insert

GluCl beta (glc-2 Nematode)

Use

Tags

ECFP

Expression

Mammalian

Mutation

Codon optimized cDNA, Y182F

Promoter

Available since

June 8, 2007

Availability

Academic Institutions and Nonprofits only

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pNIR-Fb2E7

Plasmid

#184681

Purpose

NIR-Fb to HIV gp41

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

NIR-Fb2E7

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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Cenp-B INCENP GFP

Plasmid

#45238

Purpose

Target INCENP to centromeres via full length CENP-B

Depositor

Michael Lampson

Article

Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

Insert

Cenp-B, INCENP-Δcen, GFP

Use

Tags

GFP and full length CENP-B

Expression

Mammalian

Mutation

CEN box deleted from INCENP (aa1-46)

Promoter

Available since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only

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TNXXL-CTS68

Plasmid

#48343

Purpose

FRET reporter for Ca2+ inside primary cilia

Depositor

Takanari Inoue

Article

Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

Insert

CTS68

Use

Tags

TN-XXL

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
NSarQ27G-VMAn-FKBP

Plasmid

#70230

Purpose

Expresses N-terminal portion of alpha sarcin with Q27G mutation along with VMA intein for protein splicing

Depositor

Perry Howard

Article

Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:

Insert

alpha Sarcin (N-terminal portion)

Use

Tags

FK506- and rapamycin-binding protein (FKBP), Link…

Expression

Mammalian

Mutation

Changed Gln 27 to Gly

Promoter

CMV

Available since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only
pVH232-Tier1-PmPGK1-mRuby2

Plasmid

#169523

Purpose

Tier-1 vector encoding PmPGK1-driven mRuby2 expression (PmPGK1-mRuby2-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven monomeric red fluorescent protein mRuby2

Use

Tags

Expression

Mammalian

Mutation

Promoter

PPGK

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pmyc-GFP-TNRC6A-NLS-mut

Plasmid

#42000

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Use

Tags

EGFP and Myc

Expression

Mammalian

Mutation

R930A, R931A, R932A, R934A

Promoter

CMV

Available since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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Cenp-B-targeted Aurora B FRET sensor

Plasmid

#45232

Depositor

Michael Lampson

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

CENP-B targeted Aurora B FRET sensor

Use

Tags

Expression

Mammalian

Mutation

Promoter

t7

Available since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

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VAChT-SNAP

Plasmid

#105318

Purpose

VAChT tagged with SNAP

Depositor

Justin Taraska

Article

Martineau et al Nat Commun. 2017 Nov 10;8(1):1412. doi:

Insert

VAChT (Slc18a3 Rat)

Use

Tags

SNAP tag

Expression

Mammalian

Mutation

Promoter

Available since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-YOD1 C160S

Plasmid

#49785

Purpose

Express YOD1 C160S mutant with a N-terminal Flag-tag in mammalian cells

Depositor

Christian Schlieker

Article

Ernst et al Mol Cell. 2009 Oct 9;36(1):28-38. doi: 1

Insert

YOD1 (YOD1 Human)

Use

Tags

Flag-tag

Expression

Mutation

C160S

Promoter

Available since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only
pAL190

Plasmid

#22279

Purpose

Expresses Tim DH-PH domains fused to mYFP-PIF6, for optogenetic recruitment

Depositor

Christopher Voigt

Article

Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

Insert

TimDHPH-20aaLinker-mYFP-PIF6APB (PIL2 Mustard Weed)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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OSCA1.2-PP-GFP

Plasmid

#135390

Purpose

Expresses OSCA1.2 in mammalian cells

Depositor

Ardem Patapoutian

Article

Jojoa-Cruz et al Elife. 2018 Nov 1;7. pii: 41845. doi: 10

Insert

OSCA1.2 (AT4G22120 Mustard Weed)

Use

Tags

EGFP tag connected to the gene via a precision pr…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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Mammalian_SaAID3S

Plasmid

#188651

Purpose

Expresses SaAID3S and gRNA in mammalian cells

Depositor

Keiji Nishida

Article

Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

Inserts

nSaCas9-AID3S--UGI
Sa-sgRNA

Use

Tags

UGI

Expression

Mammalian

Mutation

D10A for SaCas9

Promoter

EF-1a and H1

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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Mammalian_SaAID2S

Plasmid

#188652

Purpose

Expresses SaAID2S and gRNA in mammalian cells

Depositor

Keiji Nishida

Article

Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

Inserts

AID2S-nSaCas9--UGI
Sa-sgRNA

Use

Tags

UGI

Expression

Mammalian

Mutation

D10A for SaCas9

Promoter

EF-1a and H1

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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