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pcDNA 3.1(-) TRIM12C

Plasmid

#87080

Purpose

Murine homologues of human Trim5

Depositor

Jeremy Luban

Article

Lascano et al J Virol. 2015 Oct 14;90(1):308-16. doi:

Insert

TRIM12C (Trim12c Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Hygro(+)_GRHL1-L3F6H

Plasmid

#177765

Purpose

Overexpression of 3xFLAG-6xHis tagged human GRHL1

Depositor

Michael Ristow

Article

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

GRHL1 (GRHL1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLIPE-10u

Plasmid

#13542

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E A207R

Use

Tags

ECFP and Venus

Expression

Mammalian

Mutation

A207R in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-2xStrep-BFLF1

Plasmid

#162626

Purpose

Expresses N-terminal 2x-Strep-tagged BFLF1 in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

BFLF1

Use

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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Kohinoor-Tublin/pcDNA3

Plasmid

#67780

Purpose

Tublin-targetted Kohinoor for mammalian cell expression

Depositor

Takeharu Nagai

Article

Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

Insert

Kohinoor

Use

Tags

b-Tubulin

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only
Foxf2 pcDNA HisC

Plasmid

#122121

Purpose

Expression of Foxf2 in mammalian cells

Depositor

Paul Herring

Article

Hoggatt et al J Biol Chem. 2013 Oct 4;288(40):28477-87

Insert

Foxf2 (Foxf2 Mouse)

Use

Tags

6xHis and Express

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-Strep/HA-UBE2Q2

Plasmid

#124668

Purpose

Expresses UBE2Q2 with N-terminal Strep-HA tag in mammalian cells

Depositor

Hemmo Meyer

Article

Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

Insert

UBE2Q2 (UBE2Q2 Human)

Use

Tags

2xStrep/HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-h-mmPtch1-B-HA-GFP

Plasmid

#120903

Purpose

Expresses Ptch1-B for function assays

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

ptch1 (Ptch1 Mouse)

Use

Tags

GFP and HA

Expression

Mammalian

Mutation

deleted amino acids 620-643, truncated at 1291

Promoter

CMV

Available since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRTTO-NHASt-codoptUS2

Plasmid

#135102

Purpose

Codon-optimized HSV-1 US2 with N-terminal HAStrep tag

Depositor

Markus Landthaler

Article

Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

Insert

HSV-1 US2 codon optimized

Use

Tags

HA-Strep-Strep

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(-) TRIM12A

Plasmid

#87061

Purpose

Murine homologues of human Trim5

Depositor

Jeremy Luban

Article

Lascano et al J Virol. 2015 Oct 14;90(1):308-16. doi:

Insert

TRIM12A (Trim12a Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_mitoNanobody IRES EGFP

Plasmid

#136621

Purpose

Plasmid encoding outer mitochondrial localization of the Enhancer nanobody for GFP as a SNAP-Tag-fusion and EGFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

NTOM20-SNAP-Tag-EnhancerNb (IRES EGFP)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-p97-AE-mycStrep

Plasmid

#31841

Depositor

Hemmo Meyer

Article

Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

Insert

p97

Use

Tags

Strep and myc

Expression

Bacterial and Mammalian

Mutation

A232E

Promoter

CMV

Available since

Oct. 20, 2011

Availability

Academic Institutions and Nonprofits only
pcDNAI Tat C30G HA (P#38)

Plasmid

#14656

Depositor

Matija Peterlin

Article

Cujec et al Mol Cell Biol. 1997 Apr . 17(4):1817-23.

Insert

Tat (tat HIV)

Use

Tags

HA

Expression

Mammalian

Mutation

C30G C34G

Promoter

Available since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
Mouse NCKX6 (pcDNA3.1+)

Plasmid

#75215

Purpose

Mammalian expression of SLC8B1

Depositor

Jonathan Lytton

Article

Cai et al J Biol Chem. 2004 Feb 13;279(7):5867-76.

Insert

SLC8B1 (Slc8b1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pY123 (pcDNA3.1-huCMaCpf1)

Plasmid

#92299

Purpose

Expression of humanized CMaCpf1

Depositor

Feng Zhang

Insert

huCMaCpf1

Use

Tags

NLS and NLS-3xHA

Expression

Mammalian

Mutation

Promoter

Available since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pY125 (pcDNA3.1-huBfCpf1)

Plasmid

#92301

Purpose

Expression of humanized BfCpf1

Depositor

Feng Zhang

Insert

huBfCpf1

Use

Tags

NLS and NLS-3xHA

Expression

Mammalian

Mutation

Promoter

Available since

June 22, 2017

Availability

Academic Institutions and Nonprofits only

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pY119 (pcDNA3.1-huLb5Cpf1)

Plasmid

#92295

Purpose

Expression of humanized Lb5Cpf1

Depositor

Feng Zhang

Insert

huLb5Cpf1

Use

Tags

NLS and NLS-3xHA

Expression

Mammalian

Mutation

Promoter

Available since

June 22, 2017

Availability

Academic Institutions and Nonprofits only

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pY115 (pcDNA3.1-huMlCpf1)

Plasmid

#92275

Purpose

Expression of humanized MlCpf1

Depositor

Feng Zhang

Insert

huMlCpf1

Use

Tags

NLS and NLS-3xHA

Expression

Mammalian

Mutation

Promoter

Available since

June 22, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sACE2v3-sfGFP

Plasmid

#145174

Purpose

Mammalian expression plasmid for sACE2-sfGFP high affinity variant 3

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Use

Tags

Linker GSGGSGSGG and superfolder GFP

Expression

Mammalian

Mutation

Extracellular, soluble protease domain (a.a. M1-D…

Promoter

CMV

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-UBXD1-Strep-HA

Plasmid

#113476

Purpose

Expression of human UBXD1 with C-terminal strep-HA tag

Depositor

Hemmo Meyer

Article

Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

Insert

UBXD1 (UBXN6 Human)

Use

Tags

2xstrep-HA

Expression

Mammalian

Mutation

C174T silent mutation, wildtype without stop codon

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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