Addgene

Showing: 2921 - 2940 of 4607 results

Results per page:

pDF0159 pCMV - huDisCas7-11 mammalian expression

Plasmid

#172507

Purpose

Encodes human codon optimized DisCas7-11 protein alone for expression in mammalian cells

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

pCMV - huDisCas7-11 mammalian expression

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pDF0237 pCMV - dead huDisCas7-11 - NES - huADAR2

Plasmid

#172510

Purpose

Encodes human codon optimized catalytically dead DisCas7-11-NES-huADAR2 fusion protein alone for expression in mammalian cells

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

pCMV - dead huDisCas7-11 - NES - huADAR2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pDF0352 HvsCas7-11 with C-term Nes mammalian expression

Plasmid

#172516

Purpose

Encodes human codon optimized HvsCas7-11 protein with C-terminal NES tag for expression in mammalian cells

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

C-term Nes HvsCas7-11 mammalian expression

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pSL3718 (pQCascade_Pse)

Plasmid

#200899

Purpose

Expresses human codon-optimized Pseudoalteromonas Cas7, Cas8, Cas6, and TniQ from a CMV promoter, in a polycistronic vector format separated by T2A sequences.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

PseCas7, PseCas8, PseCas6, PseTniQ

Use

Tags

NLS (TniQ) and NLS, T2A (Cas7, Cas8, Cas6)

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
1BR9-AtWUSSTOP

Plasmid

#202053

Purpose

E. coli expression vector for N-ter GFP11 tagged Arabidopsis thaliana WUSCHEL

Depositor

Markita Landry

Article

Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

Insert

AtWUS E. Coli Codon Optimized (WUS Mustard Weed)

Use

Tags

6xHis and GFP11

Expression

Bacterial

Mutation

Promoter

T7 Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
GB1-His-TEV-A7 (2-180)

Plasmid

#190066

Purpose

Codon-optimized bacterial expression plasmid. Expresses Annexin A7 proline rich domain.

Depositor

Lalit Deshmukh

Article

Yu et al Biochemistry. 2023 Oct 3. doi: 10.1021/a

Insert

Annexin A7 PRD (ANXA7 Human)

Use

Tags

GB1-His-TEV

Expression

Bacterial

Mutation

PRD only, residues 2-180

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
GB1-His-TEV-A7-Strep (2-180)

Plasmid

#190067

Purpose

Codon-optimized bacterial expression plasmid. Expresses Annexin A7 proline rich domain with strep.

Depositor

Lalit Deshmukh

Article

Yu et al Biochemistry. 2023 Oct 3. doi: 10.1021/a

Insert

Annexin A7 PRD (ANXA7 Human)

Use

Tags

GB1-His-TEV and Strep

Expression

Bacterial

Mutation

PRD only, residues 2-180

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
1BR9-AtWUS

Plasmid

#193865

Purpose

E. coli expression vector for N-ter GFP11 and C-ter R9 tagged WUSCHEL protein from Arabidopsis thaliana.

Depositor

Markita Landry

Article

Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

Insert

AtWUS E. Coli Codon Optimized

Use

Tags

6xHis, GFP11, and R9

Expression

Bacterial

Mutation

Promoter

T7 Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pHS1184

Plasmid

#205965

Purpose

NZ_CP025815 His14-TwinStrep-bdSUMO tagged DinG HNH protein (E. coli codon optimized) in pET45b(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

DinG HNH

Use

Tags

14xHis-TwinStrep-bdSUMO

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPR-mcBEST

Plasmid

#209416

Purpose

Plasmid for multiplexed cytosine base editing in streptomycetes

Depositor

Tilmann Weber

Article

Whitford et al ACS Synth Biol. 2023 Aug 18;12(8):2353-2

Inserts

Codon optimized APOBEC1-nCas9-UGI fusion protein
csy4 from Pseudomonas aeruginosa PAO1

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

base editing cassette: PtipA ; sgRNAs: PkasO* an…

Availability

Academic Institutions and Nonprofits only

Enlarge
pET151-GyrA

Plasmid

#216229

Purpose

S. aureus USA300 GyrA Codon-optimized for E. coli

Depositor

Eric Brown

Article

French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

Insert

DNA gyrase

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pET151-GyrB

Plasmid

#216233

Purpose

S. aureus USA300 GyrB Codon-optimized for E. coli

Depositor

Eric Brown

Article

French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

Insert

DNA gyrase

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pET23a-H6-TEV-AncSZ

Plasmid

#214235

Purpose

Bacterial expression construct of AncSZ, an kinase domain engineered through ancestorial reconstruction of the kinase domains of both SYK and ZAP-70. For in vitro protein tyrosine phosphorylation

Depositor

Neel Shah

Article

Hobbs et al Protein Sci. 2022 Oct;31(10):e4411. doi:

Insert

Syk kinase (SYK Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Sequence gained through ancestral reconstruction …

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
LCC-ICCG_pET21b

Plasmid

#215408

Purpose

Expression construct for LCC-ICCG gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

LCC-ICCG

Use

Tags

Expression

Bacterial

Mutation

F243I/D238C/S283C/Y127G

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
M-NylC_pET28a

Plasmid

#215409

Purpose

Expression construct for M-NylC gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

M-NylC

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
NylB-SCY_pET29b

Plasmid

#215415

Purpose

Expression construct for NylB-SCY gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

NylB-SCY

Use

Tags

Expression

Bacterial

Mutation

R187S/F264C/D370Y

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
NylB'-SCY_pET21b

Plasmid

#215417

Purpose

Expression construct for NylB'-SCY gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

NylB'-SCY

Use

Tags

Expression

Bacterial

Mutation

R187S/F264C/D370Y

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
NylCA-TS_pET28a

Plasmid

#215419

Purpose

Expression construct for NylCA-TS gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

NylCA-TS

Use

Tags

Expression

Bacterial

Mutation

S111G/A137L/E263Q

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
NylCp2-TS_pET28a

Plasmid

#215421

Purpose

Expression construct for NylCp2-TS gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

NylCp2-TS

Use

Tags

Expression

Bacterial

Mutation

D122G/H130Y/D36A/E263Q

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
NylCK-TS_pET28a

Plasmid

#215423

Purpose

Expression construct for NylCK-TS gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

NylCK-TS

Use

Tags

Expression

Bacterial

Mutation

S111G/A137L

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge