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Plasmid#142115PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertASCL1 (ASCL1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV TRE3G Neo GFP-Progerin
Plasmid#118710PurposeLentiviral TET-ON inducible GFP-ProgerinDepositorInsertGFP-Progerin (LMNA Human)
UseLentiviral; Tet-on inducibleTagsGFPExpressionMammalianMutationPromoterCMV TRE3G (TET-ON)Available sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCK302
Plasmid#87768PurposeAs pCK301 (E. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest), but rhaS cloned downstream of ampR, which allows non-metabolisable inducer L-mannose to be usedDepositorInsertsPrhaBAD-sfGFP
rhaS from E. coli with its native RBS from E. coli
UseSynthetic BiologyTags6xHis TagExpressionBacterialMutationPromoterPrhaBAD rhamnose-inducible promoter from E. coli …Available sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-CBE4max-SpRYc-P2A-EGFP
Plasmid#208336PurposeCAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRYcDepositorInserthuman codon optimized CBE4max Cas9 variant named SpRYc with P2A-EGFP
UseCRISPRTagsP2A-EGFPExpressionMammalianMutationSc++ (1-1119), SpRY (1111-1368), D10APromoterCAGAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2428
Plasmid#144023PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertARID1B (ARID1B Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF3338
Plasmid#144814PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertHDAC1 (HDAC1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-ING2(pHD)
Plasmid#21589DepositorInsertGFP-ING2(pHD) (Ing2 Mouse)
UseTagsEGFPExpressionMammalianMutationcontains Ing2 plant homeodomain (PHD) amino acids…PromoterAvailable sinceJuly 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
TFORF3505
Plasmid#144981PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertKLF12 (KLF12 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF3086
Plasmid#144562PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSTAT1 (STAT1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
PLVX_SPTA1_IGF2BP1_IRES_PURO
Plasmid#100016PurposeTo over-express IGF2BP1 in Adult Erythroblasts using tissue specific promoter with IRES for puromycin selection.DepositorInsertInsulin like growth factor 2 mRNA binding protein 1 (IGF2BP1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterSPTA1 erythroid specific promoterAvailable sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-FGFR2c-V5/HIS
Plasmid#201107Purposeexpression of human FGFR2 receptor tyrosine kinase in mammalian cellsDepositorInserthuman FGFR2 receptor tyrosine kinase, variant c, full length, wildtype (FGFR2 Human)
UseTagsV5/HisExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMS-dsRed2-WT LMNA-GFP minigene
Plasmid#128230PurposeLamin A splicing reporterDepositorInsertlamin A (LMNA Human)
UseTagsGFP and SV40-driven dsRed2 to identify transfecte…ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST26 Ago2
Plasmid#59904PurposeExpresses 6xHis-Ago2 (N-termi tag) in mammalian cellsDepositorInsertArgonaute 2 (AGO2 Human)
UseTags6xHis (N-termi tag)ExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1 blast OFF 3xflag IREB2
Plasmid#169889PurposeExpress 3x flag IREB2, suppressible by doxycycline additionDepositorInsertIREB2 (IREB2 Human)
UseLentiviral; Doxycycline mediated suppressionTags3x FlagExpressionMammalianMutationSilent mutations in sgIREB2_1 and sgIREB2_2 targe…PromoterTREAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP.PrS.EED
Plasmid#125163PurposeExpresses human EED in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.DepositorInsertPolycomb protein EED (EED Human)
UseTagsHis-MBPExpressionInsectMutationPromoterPolyhedrinAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6-N-3XFLAG-Lrp6
Plasmid#123595DepositorInsertlow density lipoprotein receptor-related protein 6 (Lrp6 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3 ss-3xFLAG-hLRP5 (aa 32-1615)
Plasmid#115788PurposeExpresses human LRP5 with N-terminal 3xflag tag in mammalian cells, contains artificial signal sequence upstream of tag.DepositorInsertLRP5 (LRP5 Human)
UseTags3xflagExpressionMammalianMutationcontains silent mutation that creates an internal…PromoterCMVAvailable sinceOct. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDH-METTL14-WT
Plasmid#141112PurposeOverexpression of WT METTL14 in mammalian cells.DepositorInsertHMETTL14 (METTL14 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS Donor mClover-LMNA
Plasmid#98970PurposeDonor for endogenous N-ter tagging of hLMNA with mClover. Cas9 Homologous Recombination ReporterDepositorInsertLMNA5'UTR-mClover-LMNA5'exon1 (LMNA Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFl Strep-sumo-TEV-hAgo2 D597N
Plasmid#136236PurposeExpressing catalytically inactive human Argonaute-2 in insect cellsDepositorInserthuman Argonaute-2 (AGO2 Human)
UseTagsSterp SumoExpressionInsectMutationD597NPromoterpolyhedrinAvailable sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only