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Plasmid#202597PurposeExpress N-terminal Tobacco Etch Virus (TEV) protease cleavable Histidine Affinity Tag (HAT) (KDHLIHNVHKEEHAHAHNK) in mammalian cellDepositorTypeEmpty backboneUseTagsTEV cleavable site (ENLYFQG) and poly-histidine a…ExpressionMammalianMutationPromoterCAGAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only
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pCAGGS-N-TEV-HAT
Plasmid#202598PurposeExpress C-terminal Tobacco Etch Virus (TEV) protease cleavable Histidine Affinity Tag (HAT) (KDHLIHNVHKEEHAHAHNK) in mammalian cellDepositorTypeEmpty backboneUseTagsTEV cleavable site (ENLYFQG) and poly-histidine a…ExpressionMammalianMutationPromoterCAGAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOET3-C-TEV-Twin-Strep
Plasmid#203324PurposeGenerate the baculovirus to express N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in insect cellsDepositorTypeEmpty backboneUseTagsTEV cleavable site (ENLYFQG) and Twin-strep-tagExpressionInsectMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTP-PCR C4
Plasmid#189621PurposeCloned Reduced Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteriaDepositorInsertReduced Mitochondrial Genome of Thalassiosira pseudonana
UseSynthetic Biology; Diatom expressionTagsExpressionBacterial and YeastMutationRepeat region removed, and minor additional mutat…PromoterAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTP-PCR C2
Plasmid#189619PurposeCloned Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteriaDepositorInsertMitochondrial Genome of Thalassiosira pseudonana
UseSynthetic Biology; Diatom expressionTagsExpressionBacterial and YeastMutationMinor additional mutations described in manuscriptPromoterAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-MYO10ΔF3
Plasmid#194857PurposeExpress EGFP-MYO10 with the F3 FERM domain lobes deleted (1-1951) in mammalian cells.DepositorInsertMYO10 ΔF3 (MYO10 Human)
UseTagsEGFPExpressionMammalianMutationTruncated MYO10 construct. MYO10 amino acids 1-1…PromoterCMVAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-MYO10-FERM-ITGBD
Plasmid#194855PurposeExpress EGFP-MYO10 with mutations (S2001_F2002insA/T2009D) that interfere with ITGB binding in mammalian cells.DepositorInsertMYO10 S2001_F2002insA/T2009D (MYO10 Human)
UseTagsEGFPExpressionMammalianMutationMYO10 has the following mutations S2001_F2002insA…PromoterCMVAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot2-W556E-dsRNAres_U
Plasmid#147702PurposeInsect Expression of DmNot2-W556E-dsRNAresDepositorInsertDmNot2-W556E-dsRNAres (Rga Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLNHA-C1-HsNot2-del409-432_T
Plasmid#147625PurposeMammalian Expression of DmNot2-del409-432DepositorInsertDmNot2-del409-432 (Rga Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot2-del344-380_T
Plasmid#147629PurposeMammalian Expression of DmNot2-del344-380DepositorInsertDmNot2-del344-380 (Rga Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot2-del380-404_T
Plasmid#147630PurposeMammalian Expression of DmNot2-del380-404DepositorInsertDmNot2-del380-404 (Rga Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot2-del409-432_T
Plasmid#147631PurposeMammalian Expression of DmNot2-del409-432DepositorInsertDmNot2-del409-432 (Rga Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLNHA-C1-HsNot2-del344-380_T
Plasmid#147623PurposeMammalian Expression of DmNot2-del344-380DepositorInsertDmNot2-del344-380 (Rga Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLNHA-C1-HsNot2-del380-404_T
Plasmid#147624PurposeMammalian Expression of DmNot2-del380-404DepositorInsertDmNot2-del380-404 (Rga Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot3-F672AY676AF727A_S
Plasmid#147505PurposeMammalian Expression of HsNot3-F672AY676AF727ADepositorInsertHsNot3-F672AY676AF727A (CNOT3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot6-L34EI36E_S
Plasmid#147508PurposeMammalian Expression of HsNot6-L34EI36EDepositorInsertHsNot6-L34EI36E (CNOT6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLNHA-C1-HsNot3_1-241_K
Plasmid#146795PurposeMammalian Expression of HsNot3_1-241DepositorInsertHsNot3_1-241 (CNOT3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLNHA-C1-HsNot3_589-753_K
Plasmid#146797PurposeMammalian Expression of HsNot3_589-753DepositorInsertHsNot3_589-753 (CNOT3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmMe31B-QHTKRKKtoAAAAAAA_AC
Plasmid#148386PurposeInsect Expression of DmMe31B-QHTRKKtoAAAAAADepositorInsertDmMe31B-QHTRKKtoAAAAAA (me31B Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmMe31B-R307AK313AK314A_AC
Plasmid#148387PurposeInsect Expression of DmMe31B-R307AK313AK314ADepositorInsertDmMe31B-R307AK313AK314A (me31B Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only