EGFP-MYO10ΔF3
(Plasmid #194857)

• Purpose: Express EGFP-MYO10 with the F3 FERM domain lobes deleted (1-1951) in mammalian cells.
• Depositing Lab: Guillaume Jacquemet
• Publication: Miihkinen et al Cell Rep. 2021 Sep 14;36(11):109716. doi: 10.1016/j.celrep.2021.109716.

Backbone

• Vector backbone: EGFPC1
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MYO10 ΔF3
• Alt name: myosin X
• Species: H. sapiens (human)
• Mutation: Truncated MYO10 construct. MYO10 amino acids 1-1951 (amino acids 1952 - 2056 are deleted).
• Entrez Gene: MYO10 (a.k.a. MyoX)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: EGFPC

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The original EGFPC1-hMyoX plasmid (Addgene plasmid # 47608) was created by Emanuel Strehler.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The EGFP-MYO10ΔF3 construct was generated by replacing the wild-type MYO10-FERM domain (boundaries 1504-2056 in MYO10) with truncated MYO10 FERM domains where the F3 FERM lobe is deleted using a gene block (IDT). The gene blocks were inserted in EGFP-MYO10 (Addgene plasmid # 47608) using the PvuI and XbaI restriction sites. The final boundaries compared to full-length MYO10 are 1-1951.