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pET42b-BE3
Plasmid#87437PurposeExpresses BE3-NLS with an N-terminal His Tag (His6) for bacterial expressionDepositorInsertBE3 (Apobec1 Bacillus phage PBS2, Human, Rat)
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceJune 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 asyn WT
Plasmid#36046DepositorInsertalpha-synuclein (SNCA Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pN1-RnKIF5C(1-560)-Halo-Flag
Plasmid#188071PurposeMammalian cell expression of truncated version (aa 1-560) of rat kinesin-1 KIF5C tagged with Halo and Flag tagsDepositorInsertrat KIF5C (Kif5c Rat)
UseTagsFlag and HaloExpressionMammalianMutationaa1-560PromoterCMVAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.3 LRP5
Plasmid#115907PurposeExpresses human LRP5 in mammalian cells.DepositorInsertLRP5 (LRP5 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAW240
Plasmid#170791PurposeP1 integration cassette for AT110 Polymerase for integration onto Orthorep for Nanobody evolutionDepositorInsertp10B2-AT110
UseTagsExpressionYeastMutationPromoter10B2 (P1 specific promoter)Available sinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-E-cadherin-RFP
Plasmid#79603PurposepHAGE lentiviral vector with dsRed driven by the human E-cadherin promoter. When used in combination with FUGW-d2GFP-ZEB1 3' UTR, comprises the Z-cad dual sensor. Use 2nd generation packaging systemDepositorInsertCDH1 promoter region (CDH1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
SPDK3888(TRV-RNA2-pPEBV-MCS-AtIleu-tRNA)
Plasmid#149276PurposeModified Tobacco rattle virus RNA2 vector that could be used for expression of sgRNAsDepositorInsertModified TRV RNA2 with PEBV promoter and AtIleu-tRNA mobile RNA sequence
UseT-dna vectorTagsExpressionMutationT2318C (DNA) in PEBV coat protein sequencePromoterAvailable sinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.Ruby2sm-Flag.WPRE.SV40
Plasmid#98925PurposeAAV expression of Ruby2 spaghetti monster FLAG constructDepositorInsertRuby2-smFP-Flag
UseAAVTagsExpressionMammalianMutationPromoterCBAAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-SI-121
Plasmid#131126PurposeA-to-G base editing reporterDepositorInsertmutant EGFP
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceSept. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMH015_AAVS1_puro-pA-core-9xTetO-pEF-H2B-mCitrine-core-pRSV-H2B-mCherry
Plasmid#179437PurposeDual fluorescent reporter construct with double core HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locusDepositorInsertcoreHS4-TRE-cit-coreHS4-mch (DC)
UseTagsExpressionMammalianMutationPromoterpEF alpha, pRSVAvailable sinceFeb. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
plentiCRISPR-v2 Puro KIAA0141/DELE1_sg1
Plasmid#218524PurposesgRNA targeting human KIAA0141/DELE1DepositorInsertDELE1 (DELE1 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
plentiCRISPR-v2 Puro KIAA0141/DELE1_sg2
Plasmid#218525PurposesgRNA targeting human KIAA0141/DELE1DepositorInsertDELE1 (DELE1 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-2xFYVE
Plasmid#140050PurposeExpresses mCherry-tagged HRS 2xFYVE in mammalian cellsDepositorInserttandem HRS FYVE domain (Hgs Mouse)
UseTagsmCherryExpressionMammalianMutationtwo FYVE domains (amino acids 147-223), linked by…PromoterCMVAvailable sinceMay 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSecUAG-Evol2
Plasmid#163148PurposeExpresses machinery necessary for selenocysteine incorporation at UAG codonsDepositorInsertsallo-tRNA UTu2D
thioredoxin
Selenophosphate synthase
Selenocysteine synthase
Cysteine desulfurase
UseTagsExpressionBacterialMutationCysteine 32 switched to Selenocysteine (UAG), Cys…PromoterEM7, araBAD, native As SelD promoter, and native …Available sinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBabe_puro_DEST_Flag_PLD1
Plasmid#45268DepositorInsertPLD1 (PLD1 Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pGEX Stat6-SH2
Plasmid#46517DepositorInsertStat6 (STAT6 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 510-654PromoterTacAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 - HA-KLF4 FL
Plasmid#34593DepositorInsertKLF4 (KLF4 Human)
UseTagsHA tagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSLPB2B-ER50-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin
Plasmid#187948PurposeER50 degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.DepositorInsertER50-SpdCas9-tagRFPt-P2A-tagBFP
UseCRISPR and Synthetic BiologyTagsGGS linkerExpressionMammalianMutation6 mutations, T371A, L384M, M421G, G521R, Y537S, N…PromoterAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLEX_970_puro_DEST_V5_TLOC1 (399aa)
Plasmid#45288DepositorInsertTLOC1 (SEC62 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHR-SFFV-FLAG-TurboID-mutKIR-SQSTM1
Plasmid#223719PurposeExpresses FLAG-TurboID-mutKIR-SQSTM1DepositorInsertSQSTM1 (SQSTM1 Human)
UseLentiviralTagsTurboIDExpressionMammalianMutationMutation 347-DPSTGE-352 to 347-APSAAA-352PromoterSFFVAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-SFFV-FLAG-TurboID-dPB1-SQSTM1
Plasmid#223716PurposeExpresses FLAG-TurboID-dPB1-SQSTM1DepositorInsertSQSTM1 (SQSTM1 Human)
UseLentiviralTagsTurboIDExpressionMammalianMutationdeletion 1-119PromoterSFFVAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-SFFV-FLAG-TurboID-dUBA-SQSTM1
Plasmid#223717PurposeExpresses FLAG-TurboID-dUBA-SQSTM1DepositorInsertSQSTM1 (SQSTM1 Human)
UseLentiviralTagsTurboIDExpressionMammalianMutationdeletion 384-Stop codonPromoterSFFVAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-SFFV-FLAG-TurboID-mutLIR-SQSTM1
Plasmid#223718PurposeExpresses FLAG-TurboID-mutLIR-SQSTM1DepositorInsertSQSTM1 (SQSTM1 Human)
UseLentiviralTagsTurboIDExpressionMammalianMutationMutation 335-DDDW-338 to 335-AAAA-338PromoterSFFVAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-his-3C_Tau352
Plasmid#100092PurposeExpresses human full-length fetal tau protein with the N-terminal 3C-cleavable His6 tagDepositorInsertFetal microtubule-associated protein tau (MAPT Human)
UseTagsHis6ExpressionBacterialMutationwild type sequencePromoterT7Available sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBabe hygro 3xFLAG Dna2 wt
Plasmid#31955DepositorInserthDna2 (DNA2 Human)
UseRetroviralTags3xFLAGExpressionMammalianMutationChanged start ATG to AAA,PromoterAvailable sinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
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pENTRY-GFP
Plasmid#15301DepositorInserthrGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
SacI ML GFP Strand 11 Short
Plasmid#164121PurposeDetect the short Mitochondria-Endoplasmic reticulum contact along with the OMMGFP1-10DepositorInsertSacI ML GFP Strand 11 Short
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
SacI ML GFP Strand 11 Long
Plasmid#164120PurposeDetect the long Mitochondria-Endoplasmic reticulum contact along with the OMMGFP1-10DepositorInsertSacI ML GFP Strand 11 Long
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIR-CMV-SCN2A-Variant-1-IRES-mScarlet
Plasmid#162279PurposeEukaryotic expression of human SCN2A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.DepositorInsertSCN2A Variant 1, stabilized (SCN2A Human)
UseTagsIRES-mScarletExpressionMammalianMutationIVS intron inserted after bp4551.PromoterCMVAvailable sinceJan. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-195-3p
Plasmid#103311PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-195-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-195-3p target (MIR195 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC101-Donor
Plasmid#140630PurposeKanamycin resistance donor for ShCAST_harboring the temperature sensitive pSC101 origin of replication.DepositorInsertLE, RE, KanR, temperature sensitive pSC101 origin of replication
UseCRISPR; TransposonTagsExpressionBacterialMutationPromoterAvailable sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1(+)-NEMOc
Plasmid#189933PurposeMammalian expression of calcium sensor with ultra-high dynamics and sensitivityDepositorInsertNEMOc
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG_PSD95.FingR-eGFP-CCR5TC
Plasmid#46295DepositorInsertPSD95.FingR-eGFP-CCR5TC (DLG4 Synthetic, Human)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLenti6/V5/DEST-VPS35
Plasmid#21691DepositorInsertVacuolar Protein Sorting 35 homolog (VPS35 Human)
UseLentiviral; Invitrogen gateway destination vectorTagsV5ExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pC-BAR-YR
Plasmid#61766PurposeYeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing bar gene (for resistance against glufosinate ammonium) on transfer DNA (TDNA).DepositorInsertsBar gene
2 micron origin or replication and URA3 gene for S. cerevisiae
UseTernary vector for agrobacterium mediated transfo…TagsExpressionMutationPromotertrpC promoterAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/AUG-DAP5-3XFLAG
Plasmid#127331PurposeExpress 3xFLAG tagged DAP5 protein from AUG start codonDepositorInsertDAP5 (EIF4G2 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS31-cCARLIN
Plasmid#160928PurposeContains 10 constitutively expressed guide RNAs and CAG-GFP-CARLIN array sequence for DNA barcoding (when used in combination with Cas9)DepositorInsertCARLIN gRNAs and CAG-GFP-CARLIN array
UseMouse TargetingTagsExpressionMammalianMutationPromoterU6 promoter for gRNAs; CAG promoter for GFPAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLentiPGK Puro DEST JNKKTRClover
Plasmid#59151PurposeLentiviral vector to express JNK KTR mClover under PGK promoter (With Puromycin Resistance)DepositorInsertJNK Kinase Translocation Reporter (MAPK8 Mouse, Human)
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceSept. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX CrkL-SH2
Plasmid#46419DepositorInsertCrkl (CRKL Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 9-108PromoterTacAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mEGFP-CaMKIIa (T286D/T305A/T306A)
Plasmid#127392PurposeFluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T286D/T305A/T306A)DepositorInsertcalcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
UseTagsmEGFPExpressionMammalianMutationchanged Threonine 286 to Aspartic acid and Threon…PromoterpCAGAvailable sinceJuly 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
EGFP-E-Syt1
Plasmid#66830PurposeMammalian Expression, Bacterial ExpressionDepositorInsertE-Syt1 (ESYT1 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA4TO-SCN8A-Variant-1-IRES-mScarlet
Plasmid#162280PurposeEukaryotic expression of human SCN8A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.DepositorInsertSCN8A Variant 1, stabilized (SCN8A Human)
UseTagsIRES-mScarletExpressionMammalianMutationModified human HSPA5 intron 4 inserted after bp23…PromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMega-MaPylRS
Plasmid#200225PurposeThe plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with Nε-Boc-lysine.DepositorInsertsPyrrolysyl-tRNA synthetase
pyrrolysyl-tRNA
UseTagsnoneExpressionBacterialMutationnonePromoterproK-lacO and tacIAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hysn-flex-dsRed-shscramble
Plasmid#71383PurposeExpresses scramble shRNA Cre-dependently by flex switchDepositorInserthuman synapsin promoter-flex switch-dsRed-shscramble
UseAAV, Cre/Lox, and RNAiTagsExpressionMammalianMutationPromoterhuman synapsinAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGLOW30
Plasmid#172697PurposeC. elegans mScarlet co-injection markerDepositorInsertPmyo-2::mScarlet
UseTagsmScarletExpressionWormMutationPromotermyo-2Available sinceSept. 2, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLentiPGK Puro DEST p38KTRClover
Plasmid#59152PurposeLentiviral vector to express p38 KTR mClover under PGK promoter (With Puromycin Resistance)DepositorInsertp38 Kinase Translocation Reporter (MAPK14 Mouse, Human)
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceSept. 18, 2014AvailabilityAcademic Institutions and Nonprofits only
