-
Plasmid#184406Purposeyeast surface display of the SARS-CoV-2 Beta variant RBDDepositorInsertSARS-CoV-2 Beta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
UseTagsHA and c-MycExpressionYeastMutationK417N+E484K+N501YPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
3294_pETcon-SARS2-RBD_Omicron-BA1
Plasmid#184408Purposeyeast surface display of the SARS-CoV-2 Omicron BA.1 variant RBDDepositorInsertSARS-CoV-2 Omicron BA.1 Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
UseTagsHA and c-MycExpressionYeastMutationG339D+S371L+S373P+S375F+K417N+N440K+G446S+S477N+T…PromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-dHeFSpCas9-VP64-6xHis
Plasmid#92119PurposeExpression of dead/inactive increased fidelity HeFSpCas9-VP64-6xHis in bacterial cellsDepositorInsertdead/inactive HeFSpCas9-NLS-3xFLAG-VP64
UseCRISPRTags3xFLAG, 6xHis, NLS, and VP64ExpressionBacterialMutationD10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…PromoterT7Available sinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-JNKsub(TA)DD-YPet-NES
Plasmid#84634PurposeEncodes negative-control C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertJNKsub(TA)DD-YPet-NES
UseTags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailable sinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETM10 with rat FL Annexin A11
Plasmid#164496PurposeFor bacterial expression of rat His-tagged FL Annexin A11DepositorInsertrat FL Annexin A11 (Anxa11 Rat)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-FLAG-eSpCas9-plus
Plasmid#126774PurposeExpression of increased fidelity eSpCas9-plus in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.DepositorInserteSpCas9-plus
UseCRISPRTags3xFLAG, 6xHis, MBP, and NLSExpressionBacterialMutationK848A, R1060A; amino acids 1005-1013 replaced wit…PromoterT7Available sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
Pet22b-mCherry-TEAD4-IDR
Plasmid#166442PurposeBacterial expression of 6x His tagged mCherry-TEAD4 -IDR fusion proteinDepositorInsertmCherry, TEAD4-IDR (TEAD4 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42-SARA-V5-His6
Plasmid#67431PurposeExpression of hSara with C-term V5 and His6 tag in E. coliDepositorInsertSARA (SAR1B Human)
UseTagsHis and V5ExpressionBacterialMutationPromoterT7/lacOAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21 Catalytic Domain of MALT1
Plasmid#48970PurposeExpresses catalytic domain of MALT1 in e.coli AA 329-566DepositorInsertMALT1 (MALT1 Human)
UseTagsHisExpressionBacterialMutationContains AA 329-566PromoterT7Available sinceOct. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-22b(+)-RNase HIII
Plasmid#12683DepositorInsertRNase HIII
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET-CH2 (1192-1318)
Plasmid#99338PurposeBacterial expression of mouse CBP CH2 domainDepositorInsertCBP CH2 (1192-1318) (Crebbp Mouse)
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET15b-Fab1 (Francisella tularensis)
Plasmid#61690PurposeInducible expression of the FabI enzyme (enoyl reductase) from Francisella tularensisDepositorInsertenoyl-[acyl-carrier-protein] reductase (NADH)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET11a-DAAT-V33G/T242G
Plasmid#159278PurposeAminotransferase variant with near-native catalytic efficiency toward D-tryptophanDepositorInsertEngineered d-alanine aminotransferase
UseTags6x His tagExpressionBacterialMutationValine 33 and threonine 242 have been mutated to …PromoterT7Available sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-15b PAI-RBP
Plasmid#45390DepositorInsertPAI-RBP (SERBP1 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJune 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pETM6-At4CL-PhCHS-MsCHI
Plasmid#73392PurposeExpresses three enzymes to convert phenylpropanoic acids to flavanones in vivoDepositorInsertsAt4CL
PhCHS
MsCHI
UseTagsExpressionBacterialMutationPromoterT7Available sinceApril 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET15b/GgVcl V1-851 (aka Vh)
Plasmid#46172DepositorInsertVcl 1-851 (VCL Chicken)
UseTagsHisExpressionBacterialMutationaa 1-851 (aka Vh) head domainPromoterT7Available sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET23-PBD(65-150)-N-Cys
Plasmid#13722DepositorInsertPBD(65-150)-N-Cys (PAK1 Human)
UseTags6xHisExpressionBacterialMutationMet-Cys-Gly-PBD(65-150)-PromoterAvailable sinceMarch 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pETM6-Vv4CL-CmCHS-MsCHI
Plasmid#73402PurposeExpresses three enzymes to convert phenylpropanoic acids to flavanones in vivoDepositorInsertsVv4CL
CmCHS
MsCHI
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET21a-mAIM2-deltaPYD-GST
Plasmid#51534PurposeExpress GST-tagged HIN domain of mAIM2 in bacteriaDepositorInsertAim2 (Aim2 Mouse)
UseTagsGST and T7ExpressionBacterialMutationPromoterlac operatorAvailable sinceMarch 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28A-p53 (1-73) (P12A P13A)
Plasmid#62066PurposeExpression of human p53 (residues 1-73) (P12A, P13A) in e. coliDepositorInsertp53 (TP53 Human)
UseTagsHisExpressionBacterialMutationContains TAD residues 1-73; P12A, P13APromoterT7Available sinceFeb. 23, 2015AvailabilityAcademic Institutions and Nonprofits only