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Plasmid#103798PurposeBLInCR 'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue lightDepositorUseTagsExpressionMammalianMutationTetR: A4G (M2V)PromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only
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pcdna3 Flag Jip3b(T266A, T276A, T287A)
Plasmid#58924Purposeinteracts with MAPK signaling moduleDepositorInsertmitogen-activated protein kinase 8 interacting protein 3 (Mapk8ip3 Mouse)
UseTagsFlagExpressionMammalianMutationchanged T266A, T276A, T287APromoterAvailable sinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Laccase2 MCS-HIPK3 Exon
Plasmid#69888PurposeExpresses the human HIPK3 exon 2 circular RNA in DrosophilaDepositorInsertHIPK3 (HIPK3 Human, Fly)
UseTagsExpressionInsectMutationExpresses aa1-194 of HIPK3PromoterMetallothionein Promoter (pMT)Available sinceNov. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
psicheck2-MAIP1mut
Plasmid#203604PurposeReporter plasmid carrying MAIP1 3'UTR with mutated miR27b target sequencesDepositorInsertmatrix AAA peptidase interacting protein 1 (MAIP1 Human)
UseLuciferaseTagsExpressionMammalianMutationmiR-27b target sequences in the 3'UTR was mu…PromoterSV40Available sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 BBS W391A pcDNA3
Plasmid#206077PurposeCav2.2 calcium channel with an exofacial double Bungarotoxin Binding Site motif in domain II and a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop that prevents Beta bindingDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationW391APromoterCMVAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIRESneo2 mycBioID-Tollip M240A,F241A
Plasmid#178055Purposemyc-BioID fused to human Tollip carrying CUE domain mutations cloned into the pIRESneo2 plasmid. For generation of stable expressing mammalian cells used for proximity dependent biotin labelling.DepositorInsertToll-Interacting Protein (TOLLIP Human)
UseTagsmycBioIDExpressionMammalianMutationChanged Methionine 240 to Alanine and changed Phe…PromoterCMVAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcdna3 T7 Jip1b(471-707)(C478S, C667S and C699T)
Plasmid#52129Purposea mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.DepositorInsertJnk interacting protien (Jip1b)(471-707)(M1+2+6) (Mapk8ip1 Mouse)
UseTagsT7ExpressionMammalianMutationdeleted amino acids 1-470 and changed C478S, C667…PromoterAvailable sinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcdna3 T7 Jip1b(471-707)(C478S, C643S and C696T)
Plasmid#52130Purposea mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.DepositorInsertJnk interacting protein Jip1b(471-707)(M1+3+6) (Mapk8ip1 Mouse)
UseTagsT7ExpressionMammalianMutationdeleted amino acids 1-470 and changed C478S, C643…PromoterAvailable sinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcdna3 T7 Jip1b(471-707)(C478S, C631S and C699T)
Plasmid#52131Purposea mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.DepositorInsertJnk interacting protein (Jip1b)(471-707)(M1+4+6) (Mapk8ip1 Mouse)
UseTagsT7ExpressionMammalianMutationdeleted amino acids 1-470 and changed C478S, C631…PromoterAvailable sinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcdna3 T7 Jip1b(471-707)(C478S, C631S, C643S, C667S and C699T)
Plasmid#52133Purposea mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.DepositorInsertJNK interacting protein-1b (JIP-1b)(471-707)(M1-5) (Mapk8ip1 Mouse)
UseTagsT7ExpressionMammalianMutationdeleted amino acid 1-470 and C478S, C631S, C643S,…PromoterAvailable sinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcdna3 T7 Jip1b(282-707)(C605S, C631S, C643S and C696T)
Plasmid#52134Purposea mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.DepositorInsertJNK interacting protein-1b (JIP-1b)(282-707)(M1-5) (Mapk8ip1 Mouse)
UseTagsT7ExpressionMammalianMutationdeleted amino acid 1-281 and C605S, C631S, C643S …PromoterAvailable sinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
anxA2-GFP
Plasmid#107196PurposeExpresses human annexin A2-GFP fusion protein for live cell imagingDepositorInsertannexin A2 (ANXA2 Human)
UseTagsGreen Fluorescent ProteinExpressionMammalianMutationAla residue 65 in human sequence replaced by Glu …PromoterCMVAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLPC-MYC-hTRF1
Plasmid#64164PurposeRetroviral vector expressing human TRF1 with N-terminal MYC tagDepositorInserthTRF1 (TERF1 Human)
UseRetroviralTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceMay 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
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CIBN-GFP-Sec61B
Plasmid#104177PurposeExpresses fusion of CIB1 (1-170) with GFP and Sec61BDepositorUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FLAG-puro-PTPIP51(1-150_175-470)
Plasmid#170537PurposeExpresses PTPIP51 FFAT-like motif (151-175) deletion mutant (1-150_176-470) in human HeLa cells after transfectionDepositorInsertProtein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
UseTagsFLAG tagExpressionMammalianMutationdeleted amino acids 151-175PromoterCAG promoterAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pclbw-opa1(isoform 7DeltaS1)-myc
Plasmid#62846PurposeMammalian Expression of myc-tagged Opa1 isoform 7 Delta S1DepositorInsertmmOPA1isoform7 DeltaS1 - myc (Opa1 Mouse)
UseRetroviralTags9xMycExpressionMammalianMutationPromotercmv-bactinAvailable sinceJan. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAW-CD8-Nb127D01-GFP
Plasmid#171577PurposeExpresses GFP-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-GFP) in fly cell cytomembraneDepositorInsertCD8-Nb127D01-GFP (CXCR2 Human)
UseTagsCD8 and GFPExpressionInsectMutationPromoterfly actin5C promoterAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-hTRF2-tagRFP-T
Plasmid#103812PurposeBLInCR 'Localizer' construct that marks telomeres and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitmentDepositorUseTagsExpressionMammalianMutationhTRF2: deletion of amino acids 1-42 and 476 compa…PromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
Venus-Bik-4E-pEGFP-C1
Plasmid#166744PurposeExpress Venus fused to the N-terminus of the Bcl-2 family protein, Bik with mutation in BH3 domain to disrupt binding anti-apoptotic proteinsDepositorInsertBik-4E (BIK Human)
UseTagsVenusExpressionMammalianMutation4 hydrophobic residues in the BH3 region mutated …PromoterCMVAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only