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Plasmid#110830PurposeExpresses zinc alpha-2 glycoprotein (ZAG) and dTomato (dT) reporter where both sequences are separated by a T2A self-cleaving peptide sequence as a result, the dT is not fused with the ZAG protein.DepositorInsertAZGP1 (Azgp1 Mouse)
UseNon-viral gene expression vectorTagsT2A and dTomatoExpressionMutationPromoterCMVAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-Cas9-P2A-Puro_NFATC2-sgRNA
Plasmid#188704PurposeLentivirus transfer plasmid encoding Cas9, puromycin resistance, and 4 sgRNAs targeting human NFATC2DepositorInsertNFATC2 sgRNAs
UseLentiviralTagsExpressionMammalianMutationPromoterhUbCAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-retro-puro-tetO-CTR1-shRNA
Plasmid#53180PurposeExpresses shRNA against human CTR1 from puromycin resistance retroviral vectorDepositorInsertCTR1 (SLC31A1 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
N111 nLV EF-1a-PGK-Puro Gal4-HP1a FL
Plasmid#43968DepositorInsertCbx5 (Cbx5 Mouse)
UseLentiviralTagsGal4/DBDExpressionMutationPromoterEF-1aAvailable sinceJune 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Cb5-IRES-puromycin-pLVx-EF1a
Plasmid#177434PurposeTo express mCherry fused to Cb5 endoplasmic reticulum (ER) targeting sequence. Lentiviral vector used to make cell lines expressing this ER landmark.DepositorInsertCb5 tail anchor (Cyb5r4 Rat)
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
MSCV-BRD9_BET-PGK-Puro-IRES-GFP
Plasmid#75120PurposeRetroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the first bromodomain of BRD4DepositorInsertBRD9 (BRD9 Human)
UseRetroviralTagsExpressionMutationBromodomain replaced with the first bromodomain o…PromoterAvailable sinceMay 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
NSP9-1x-BioID2 pBabe-puro (SARS-CoV-2)
Plasmid#186003PurposeExpresses SARS-CoV-2 NSP9 fused to BioID2 and HA tagDepositorInsertSARS-CoV-2 NSP9 (ORF1ab SARS-CoV-2)
UseRetroviralTagsBioID2 and HAExpressionMammalianMutationPromoterLTRAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-BE3RA-P2A-GFP-PGK-Puro
Plasmid#110868PurposeLentiviral vector for constitutive expression of BE3RA-P2A-GFP in mammalian cells (codon optimized)DepositorInsertBE3RA
UseLentiviralTagsExpressionMutationD10APromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
XLone-Puro Cas13d P2A eGFP-NLS
Plasmid#155182PurposePiggybacTransposon-based tunable and temporal expression control of Cas13d and nuclear eGFPDepositorInsertCasRx
UseUnspecified; Piggybac transposonTagsNLS and P2A eGFP NLSExpressionMammalianMutationPromoterTRE3GAvailable sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-BICRAL-FLAG-pgk-puro
Plasmid#107732PurposeTet inducible lentiviral C-terminal FLAG BICRAL (GLTSCR1L) ORFDepositorInsertBICRAL (BICRAL Human)
UseLentiviralTagsFLAGExpressionMutationPromoterCMVAvailable sinceMarch 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-FLEX-NEUROD1-T2A-mCherry
Plasmid#178584PurposeCre-dependent expression of NEUROD1 and mCherry under the constitutive promoterDepositorInsertNEUROD1 (NEUROD1 Human)
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CB-FLPe-P2A-copGFP-T2A-Puro
Plasmid#72258PurposeExpress FLPe, copGFP and Puromycin resistance gene under CB promoterDepositorInsertFLPe-P2A-copGFp-T2A-Puro
UseLentiviralTagsExpressionMammalianMutationPromoterCB and chicken beta-actin + CMV enhancerAvailable sinceJan. 15, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
AAVS1-Puro CAG J591-scFV T-CAR
Plasmid#171961PurposeDonor plasmid for J591 T-CAR expression in human cellsDepositorInsertSynthetic J591-scFV CAR
UseCRISPR, Synthetic Biology, and TALENTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
PEA1-Nuclease-Puro HEK3 CTT ins
Plasmid#171995PurposeDelivers all prime editing nuclease components targeting the HEK3 gene for a CTT inserrtion, in a single, puromycin selectable plasmidDepositorInsertHEK3 CTT insertion pegRNA and CbH-Cas9-RT-T2A-Puro, hU6-pegRNA HEK3 CTT ins, hU6-sgRNA sham
UseTagsExpressionMammalianMutationPromoterCMV for Cas9, U6 for gRNAsAvailable sinceSept. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF2α P405A/P531A/N847Q-pBabe-Puro
Plasmid#25948DepositorInsertHypoxia inducible factor 2 alpha (EPAS1 Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationcDNA changed amino acids: Proline 405 to Alanin…PromoterAvailable sinceSept. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK Puro GFP-GABARAPL1 G116
Plasmid#123243PurposeExpression vector with PGK promoter for low expression of EGFP-GABARAPL1 G116. For lentivirus production and stable transduction in mammalian cells.DepositorInsertGamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationDeleted amino acid 117. Stop codon after G116PromoterPGKAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CB-Nluc-P2A-copGFP-T2A-Puro
Plasmid#73039PurposeExpress Nluc, copGFP dual reporterDepositorInsertNluc-P2A-copGFP-T2A-Puro
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterchicken beta-actin + CMV enhancer and chicken bet…Available sinceJan. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV[Tet]-Puro-TRE3G>mMyod1[NM_010866.2]
Plasmid#184380PurposeThe mouse Myod1 gene is expressed under a doxcycline-inducible TRE3G promoterDepositorInsertMyod1 (Myod1 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSCV-BRD9_N216A-PGK-Puro-IRES-GFP
Plasmid#75116PurposeRetroviral expression plasmid encoding a human BRD9 mutant carrying the N216A single amino acid mutationDepositorInsertBRD9 (BRD9 Human)
UseRetroviralTagsExpressionMutationsingle amino acid mutation N216APromoterAvailable sinceMay 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV-EZH2_EZH1SET-PGK-Puro-IRES-GFP
Plasmid#75126PurposeRetroviral expression plasmid encoding a human EZH2 SET-domain-swap mutant containing the EZH1 SET domainDepositorInsertKMT6 (EZH2 Human)
UseRetroviralTagsNoneExpressionMutationSET domain replaced with the SET domain of EZH1PromoterAvailable sinceMay 23, 2016AvailabilityAcademic Institutions and Nonprofits only