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pDestTol2CG2-Neurog1-Magneto2.0-p2A-mCherry-pA

Plasmid

#74302

Purpose

Expression of Magneto2.0-p2A-mCherry in Rohon-Beard sensory neurons of the zebrafish

Depositor

Ali Guler

Article

Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

Insert

Magneto2.0 (FTL, FTH1, Trpv4 Rat, Human)

Use

Zebrafish

Tags

FLAG, cmcl2::EGFP, and p2A-mCherry

Expression

Mutation

TRPV4: delta 760-871

Promoter

Neurog1

Available since

May 11, 2016

Availability

Academic Institutions and Nonprofits only

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Str-STIM1-NN_ST-SBP-mCherry

Plasmid

#65263

Purpose

synchronize trafficking of ST from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Str-STIM1-NN and truncated sialyltransferase (ST6GAL1 Human)

Use

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Mutation

contains only amino acids 1 to 43 of ST

Promoter

CMV

Available since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyso-mCherry-Rheb(C181S)

Plasmid

#192438

Purpose

Encodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant targeted to lysosome membrane.

Depositor

Jin Zhang

Article

Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

Insert

Lyso-mCherry-Rheb(C181S) (RHEB Human)

Use

Tags

Full-length LAMP1 and mCherry

Expression

Mammalian

Mutation

Rheb Cys 181 mutated to Ser; abolishes C-terminal…

Promoter

CMV

Available since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pET-45b-RUNX2(WT)-IDR-mCherry

Plasmid

#145286

Purpose

Bacterial Expression of 6xHis RUNX2(WT) IDR mCherry Fusion Protein

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

RUNX2(WT)-IDR (RUNX2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pHage2-EF1a-mCherryTRF1-IRES-Blast

Plasmid

#182588

Purpose

expresses mcherry-trf1 in mammalian cells

Depositor

Agnel Sfeir

Article

Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

Insert

hTRF1 (TERF1 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-Cry2-mCherry-coiled coil

Plasmid

#166448

Purpose

Expresses fusion of CRY2PHR with mCherry and YAP Coiled-coil domain

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

Cry2, mCherry, coiled coil(298-359) (CRY2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-Cry2-mCherry- YAP-C-IDR

Plasmid

#166447

Purpose

Expresses fusion of CRY2PHR with mCherry and YAP-C-IDR

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

Cry2, mCherry, YAP-C-IDR(266-504) (YAP1, CRY2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-Cry2-mCherry-TEAD4-IDR

Plasmid

#166449

Purpose

Expresses fusion of CRY2PHR with mCherry and TEAD4-IDR

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

Cry2, mCherry, TEAD4-IDR(89-230) (TEAD4, CRY2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCW57-mCherry-2A-BRD4 Iso AdelCTD

Plasmid

#137722

Purpose

Doxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 long isoform missing its C-terminal, P-TEFb interacting domain (CTD)

Depositor

Scott Floyd

Article

Edwards et al Cell Rep. 2020 Sep 22;32(12):108166. doi

Insert

BRD4 long isoform without its C-terminal, P-TEFb interacting domain (BRD4 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Truncated at amino acid 1328

Promoter

Tight TRE promoter

Available since

Jan. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pSLQ1869-1 pHR: U6-SpsgSV40 CMV-mCherry

Plasmid

#84249

Purpose

Expresses Sp sgSV40 gRNA with a mCherry fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sp sgSV40

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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hSyn-GW-dCas9-SET2-IRES2-mCherry

Plasmid

#202089

Purpose

Neuron-specific expression vector of dCas9-Set2

Depositor

Elizabeth Heller

Article

Xu et al Neuron. 2021 Sep 15;109(18):2943-2966.e8

Insert

dCas9-Set2

Use

Tags

3x FLAG and mCherry

Expression

Mammalian

Mutation

Promoter

human Synapsin

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-Myr-DnaJB1-PKAcat-mCherry

Plasmid

#181853

Purpose

mCherry-tagged DnaJB1-PKA catalytic subunit fusion with N-terminal myristoylation site added; for mammalian expression.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

Myr-DnaJB1-PKAcat-mCherry (PRKACA, DNAJB1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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PDZb-beta2(616-951)-mCherry

Plasmid

#100736

Purpose

To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

AP2B1 (AP2B1 Human)

Use

Tags

PDZb and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hWWTR1-W2B)-PGKpuro2AmCherry-W

Plasmid

#163177

Purpose

Lentiviral gRNA plasmid targeting human WWTR1 gene, co-expression of mCherry tag

Depositor

Sok Ching Cheong

Article

Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

Insert

WWTR1 (WWTR1 Human)

Use

CRISPR and Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-NOS-IN133.3xFLAG.mCherry-WPRE

Plasmid

#127868

Purpose

pAAV plasmid expressing an NOS-IN133.3xFLAG.mCherry fusion protein under the hSyn promoter

Depositor

Florian Freudenberg

Article

Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

Insert

Nos1 (Nos1 Mouse)

Use

AAV

Tags

3xFLAG and mCherry

Expression

Mutation

Amino acids 1-133 only

Promoter

hSyn

Available since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pHR-mCherry-P2A-strepII-PPPCB

Plasmid

#67923

Purpose

Expresses mCherry and strepII-tag-PP2aBeta in mammalian cells

Depositor

Ron Vale

Article

Tanenbaum et al Elife. 2015 Aug 25;4. doi: 10.7554/eLife

Insert

mCherry-P2A-StrepII-PP2aBeta (Ppp2cb Rat)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pJK148-Pnmt41-GBP-mCherry(C)-leu1+

Plasmid

#89069

Purpose

genomic GBP-mCherry epitope C-terminal tagging at leu1+ locus in S. pombe

Depositor

Quanwen Jin

Article

Chen et al J Cell Sci. 2017 Jan 12. pii: jcs.198457

Type

Empty backbone

Use

Genomic integration in s. pombe

Tags

GBP-mCherry

Expression

Yeast

Mutation

Promoter

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pJPC12-ΔM13-mCherry-P/PM,5G6G-GFP

Plasmid

#80911

Purpose

Reporter plasmid (mCherry and GFP)

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

mCherry
GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

P5G6G and PM,5G6G

Available since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-Zdk1-RhoA Q63L

Plasmid

#81058

Purpose

mammalian expression of Zdk1 tagged RhoA Q63L

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Inserts

Zdk1
RhoA Q63L (RHOA Human)

Use

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pAT15415-BEAR-GFP-target-mCherry

Plasmid

#162995

Purpose

plasmid expressing an sgRNA targeting the BEAR-GFP plasmid along with an mCherry marker

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

sgRNA targeting the BEAR-GFP plasmid

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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