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pcDNA-Flag-H3.3-polyA

Plasmid

#59780

Purpose

Express Flag-tagged H3.3 (H3F3B) in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

H3F3B (H3-3B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Casp2 C303A-Flag

Plasmid

#11812

Depositor

Guy Salvesen

Insert

Caspase-2 catalytic mutant (CASP2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

C303A

Promoter

Available since

May 12, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA5-FRT/TO-FLAG-SHLD3

Plasmid

#114123

Purpose

Expresses N-terminally tagged FLAG-SHLD3 in mammalian cells

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

SHLD3 (SHLD3 Human)

Use

Genomic integration, tetracycline inducible

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV/TetO2

Available since

Aug. 16, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-Flag-Tet1 CD Mut

Plasmid

#72218

Purpose

Express flag-tagged mouse Tet1 catalytic domain - catalytic dead mutant.

Depositor

Yi Zhang

Article

Ito et al Science. 2011 Sep 2;333(6047):1300-3. do

Insert

Ten-Eleven Translocation 1 (Tet1 Mouse)

Use

Tags

flag

Expression

Mammalian

Mutation

The plasmid expresses TET1 catalytic domain (cata…

Promoter

CMV

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only
pcDNA4-FLAG-Jhdm2a-H1122A

Plasmid

#38140

Depositor

Toru Nakano, Toshinobu Nakamura

Article

Nakamura et al Nature. 2012 Jun 3;486(7403):415-9. doi:

Insert

Jhdm2a-H1122A (Kdm3a Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

protein starts at aa115; H1122A

Promoter

CMV

Available since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only

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3xFLAG-dCas9/pTEF1p-CYC1t

Plasmid

#62190

Purpose

Expresses 3xFLAG-dCas9 in budding yeast for enChIP analysis to purify specific genomic regions of interest.

Depositor

Hodaka Fujii

Article

Fujii et al Biol Methods Protoc. 2022 Oct 17;7(1):bp

Insert

3xFLAG-dCas9

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Yeast

Mutation

human codon-optimized, D10A, H840A

Promoter

TEF1 promoter

Available since

Feb. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pHR- flag-Lag16-JAM-B

Plasmid

#205200

Purpose

This vector encodes of the synCAM (JAM-B) and GFP nanobody LAG16

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM JAM-B and Lag16 anti-GFP nanobody

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK-flag-USP19 C506S

Plasmid

#78584

Purpose

Expression flag-USP19 C506S in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-USP19 C506S (USP19 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

mutate 506 cysteine residue to serine

Promoter

CMV

Available since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
FLAG-FullLength-TET1-CXXC(24)

Plasmid

#124398

Purpose

Full-length TET1 missing zinc finger domain (585-624) used to PiggyBAC rescue the function of TET1/2 DKO cells

Depositor

Shaun Cowley

Article

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

FLAG-FullLength-TET1 with CXXC zinc finger deletion (585-624) (TET1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

CXXC zinc finger deletion (aa 585-624)

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pX330-Flag-wtSpCas9-H840A

Plasmid

#80453

Purpose

SpCas9 mammalian expression vector

Depositor

Ervin Welker

Article

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Insert

SpCas9_H840A

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

Promoter

CBh

Available since

Aug. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA3-Flag MKK6(K82A)

Plasmid

#13519

Depositor

Roger Davis

Article

Raingeaud et al Mol Cell Biol. 1996 Mar . 16(3):1247-55.

Insert

MKK6(K82A) (MAP2K6 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Lys-82Ala

Promoter

Available since

July 25, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/T0-Flag-CIP2A

Plasmid

#222623

Purpose

mammalian expression vector of Flag-tagged CIP2A

Depositor

Daniel Durocher

Article

Adam et al Nat Cancer. 2021 Dec;2(12):1357-1371. do

Insert

CIP2A (CIP2A Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.3 ss-FLAG-AP-hNDP (aa 25-133)

Plasmid

#115789

Purpose

Expresses and secretes human Norrin with N-terminal flag and alkaline phosphatase tags, contains artificial signal sequence upstream of tags.

Depositor

Harald Junge

Article

Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

Insert

NDP (NDP Human)

Use

Tags

alkaline phosphatase and flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Flag-hsDicer (D1709A)

Plasmid

#41588

Depositor

Phil Sharp

Article

Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

Insert

hsDicer (DICER1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

D1709A

Promoter

CMV

Available since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only
3'-3xFLAG minimal CMV pCDNA5

Plasmid

#212045

Purpose

LR destination vector for integration into N2a rtTA3 expressing cells with an FRT site in the Rosa26 locus

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-FLAG-APEX2-LGALS3

Plasmid

#175756

Purpose

Lentiviral vector expressing N-terminally tagged APEX2-FLAG-LGALS3

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

LGALS3 (LGALS3 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX Flag RagC T90N

Plasmid

#112758

Purpose

express Flag-RagC (T90N mutant)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Flag-RagC (T90N) (RRAGC Human)

Use

Lentiviral

Tags

Flag

Expression

Mutation

T90N

Promoter

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe-Flag-Daxx-Puro

Plasmid

#124898

Purpose

Express Flag-Daxx in mamalian cells

Depositor

Xiaolu Yang

Article

Tang et al PLoS One. 2013;8(2):e55813. doi: 10.1371

Insert

Death domain-associated protein 6 (DAXX )

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-FLAG-MAGE-2-429

Plasmid

#89090

Purpose

mammalian expression of full-length human MAGE-A11 with N-terminal FLAG tag

Depositor

Elizabeth Wilson

Article

Bai et al Mol Cell Biol. 2005 Feb;25(4):1238-57.

Insert

MAGE-A11 (MAGEA11 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 6, 2017

Availability

Academic Institutions and Nonprofits only
pSLIK 3xFLAG-MAVS hygro

Plasmid

#158634

Purpose

Lentiviral expression vector for an inducible 3xFLAG-tagged MAVS construct

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

MAVS (MAVS Human)

Use

Lentiviral

Tags

3XFLAG

Expression

Mutation

Promoter

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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