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Plasmid#205310PurposeProduction of AAV particles encoding WHaloCaMP1a from the CaMKII promoterDepositorInsertWHaloCaMP1a
UseAAVTagsExpressionMutationPromoterAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-WHaloCaMP1a-EGFP
Plasmid#205309PurposeProduction of AAV particles encoding WHaloCaMP1a-EGFP from the CaMKII promoterDepositorInsertWHaloCaMP1a-EGFP
UseAAVTagsExpressionMutationPromoterAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJT282 Lenti pEF-dCas9-3XFLAG-N+Z+F-T2A-tagBFP-P2A-Blast
Plasmid#187337PurposedCas9 effector fusion for manipulating transcriptionDepositorArticleInsertdCas9-3XFLAG-N+Z+F-T2A-tagBFP-P2A-Blast
UseCRISPR and LentiviralTags3XFLAGExpressionMammalianMutationPromoterpEF1aAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAR013 Lenti pEF-dEnAsCas12a-NLS-VP64-3XHA-T2A-BSD
Plasmid#195547PurposedCas12a effector fusion for manipulating transcriptionDepositorArticleInsertLenti pEF-dEnAsCas12a-NLS-VP64-3XHA-T2A-BSD
UseCRISPR and LentiviralTags3XHAExpressionMammalianMutationPromoterpEF1aAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
lentiTet-OTX2-P2A-mCherry BlastR
Plasmid#186741PurposeDox inducible OTX2-P2A-mCherry expression construct cloned into a lentiviral backbone containing a blasticidin resistance geneDepositorInsertOTX2 (OTX2 Human)
UseLentiviralTagsP2A-mCherryExpressionMammalianMutationPromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHDR_NANOS3-T2A-mVenus-PuroTK
Plasmid#222903PurposeHomology directed repair template for knocking in mVenus reporter to NANOS3.DepositorInsertmVenus
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIR-CMV-SCN2A-Variant-4-IRES-mScarlet
Plasmid#209410PurposeExpression of wild-type neonatal isoform of human SCN2A.DepositorInsertSCN2A Variant 4, stabilized (SCN2A Human)
UseTagsExpressionMammalianMutationIVS intron inserted after bp 4551 of the SCN2A OR…PromoterCMVAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV hU6-gRNA hUbC-VP64-dSaCas9-VP64-T2A-Thy1.1
Plasmid#194279PurposeExpresses gRNA and VP64-dSaCas9-VP64 from lentiviral vectorDepositorInsertshumanized VP64 dSaCas9 VP64 T2A Thy1.1
gRNA
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterhU6 and hUbCAvailable sinceFeb. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-iPE-Plus
Plasmid#214062Purposeinducible PE-Plus system for controllable prime editing; this plasmid is used to insert PE-Plus prime editor (PEmax-P2A-hP53DD-IRES-MLH1dn) in one allele of AAVS1 locusDepositorInsertPE-Plus
UseTagsExpressionMammalianMutationPromoterTRE-tightAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJT259 Lenti pEF-dCas9-3XFLAG-VP64-T2A-tagBFP-P2A-Blast
Plasmid#187335PurposedCas9 effector fusion for manipulating transcriptionDepositorArticleInsertCas9-3XFLAG-VP64-T2A-tagBFP-P2A-Blast
UseCRISPR and LentiviralTags3XFLAGExpressionMammalianMutationPromoterpEF1aAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-synapsin-WHaloCaMP1a
Plasmid#205308PurposeProduction of AAV particles encoding WHaloCaMP1a from the synapsin promoterDepositorInsertWHaloCaMP1a
UseAAVTagsExpressionMutationPromoterAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFIa-DIO-LMO7 (mNeonGreen-eKL9h-VChR1)
Plasmid#205098PurposeCre-dependent fusion protein of mNeonGreen, eKL9h, and Volvox channelrhodopsin-1 for bioluminescent optogeneticsDepositorInsertsmNeonGreen
eKaz luciferase
Volvox Channelrhodopsin 1
UseAAVTagsExpressionMutationPromoterAvailable sinceSept. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Puro-moxGFP-LMNB1
Plasmid#207773PurposeDonor template for Puro-2A-moxGFP insertion into the N-terminus of the LMNB1 locus for nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770DepositorArticleInsertLMNB1 Homology Arms flanking a Puro-moxGFP Cassette (LMNB1 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHR-SFFV-FLAG-TurboID-mutKIR-SQSTM1
Plasmid#223719PurposeExpresses FLAG-TurboID-mutKIR-SQSTM1DepositorInsertSQSTM1 (SQSTM1 Human)
UseLentiviralTagsTurboIDExpressionMammalianMutationMutation 347-DPSTGE-352 to 347-APSAAA-352PromoterSFFVAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-SFFV-FLAG-TurboID-dPB1-SQSTM1
Plasmid#223716PurposeExpresses FLAG-TurboID-dPB1-SQSTM1DepositorInsertSQSTM1 (SQSTM1 Human)
UseLentiviralTagsTurboIDExpressionMammalianMutationdeletion 1-119PromoterSFFVAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-SFFV-FLAG-TurboID-dUBA-SQSTM1
Plasmid#223717PurposeExpresses FLAG-TurboID-dUBA-SQSTM1DepositorInsertSQSTM1 (SQSTM1 Human)
UseLentiviralTagsTurboIDExpressionMammalianMutationdeletion 384-Stop codonPromoterSFFVAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-SFFV-FLAG-TurboID-mutLIR-SQSTM1
Plasmid#223718PurposeExpresses FLAG-TurboID-mutLIR-SQSTM1DepositorInsertSQSTM1 (SQSTM1 Human)
UseLentiviralTagsTurboIDExpressionMammalianMutationMutation 335-DDDW-338 to 335-AAAA-338PromoterSFFVAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
MTOR-F2108L_CD19-CAR-28z-2A-EGFP_AAV6_Donor
Plasmid#211904PurposeVector for AAV6 donor production. Targeted CD19-CAR-28z-2A-EGFP transgene integration to the MTOR locus with the dominant rapamycin resistance MTOR-F2108L mutation via homology-directed repair.DepositorInsertCD19-CAR-28z-2A-EGFP
UseAAVTagsExpressionMammalianMutationPromoterhPGK1Available sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMZ7
Plasmid#223180PurposepORI28 derivative with lacZ and upp gene inserted into the backbone; for generating gene-specific knockouts.DepositorTypeEmpty backboneUseChromosomal integrationTagsExpressionMutationPromoterAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Puro-mScarlet-ACTB
Plasmid#207754PurposeDonor template for Puro-2A-mScarlet insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748DepositorArticleInsertACTB Homology Arms flanking a Puro-mScarlet Cassette (ACTB Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits