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AT3G14840 X033_pECIA14

Plasmid

#114863

Purpose

Prey vector AT3G14840 X033_pECIA14 should be used with bait vector AT3G14840 X033_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT3G14840 (AT3G14840 Mustard Weed)

Use

Tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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NIK1 A11_pECIA14

Plasmid

#114777

Purpose

Prey vector NIK1 A11_pECIA14 should be used with bait vector NIK1 A11_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT5G16000 (NIK1 Mustard Weed)

Use

Tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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NIK3 nA5_pECIA14

Plasmid

#114769

Purpose

Prey vector NIK3 nA5_pECIA14 should be used with bait vector NIK3 nA5_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G60800 (NIK3 Mustard Weed)

Use

Tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pET3d-SF-DCTN1-TS

Plasmid

#169375

Purpose

E. coli expression plasmid for human DCTN1 (longest transcript variant p150glued/isoform 1), with an N-terminal splitFlAsH tag and a C-terminal TwinStrep tag.

Depositor

Bernd Bukau

Article

Bertolini et al Science. 2021 Jan 1;371(6524):57-64. doi

Insert

dynactin subunit 1 isoform 1 (DCTN1 Human)

Use

Tags

6xHis Tag, SplitFlAsH Tag, and TwinStrep Tag

Expression

Bacterial

Mutation

Point mutations: A139T, I304T, Q322R, T516A, E577…

Promoter

Available since

May 27, 2021

Availability

Academic Institutions and Nonprofits only

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Venus-tBid-2E-pEGFP-C1

Plasmid

#166747

Purpose

Express Venus fused to the N-terminus of the Bcl-2 family protein, tBid with mutation to 2 positions in the BH3 domain

Depositor

David Andrews

Article

Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

Insert

tBid-2E (Bid Mouse)

Use

Tags

Venus

Expression

Mammalian

Mutation

2 hydrophobic residues in the BH3 region mutated …

Promoter

CMV

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5-eGFP-FRT-T0-RNF168 delta MIU1/MIU2

Plasmid

#133980

Purpose

inducible mammalian expression vector of eGFP tagged RNF168 where both motifs interacting with ubiquitin have been deleted

Depositor

Daniel Durocher

Article

Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

Insert

RNF168 (RNF168 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

deletion of MIU1 and MIU2

Promoter

CMV

Available since

Nov. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pCAGGS SARS-CoV-2 BA.2.75 Spike

Plasmid

#190012

Purpose

Expresses codon-optimized full length SARS-CoV-2 BA.2.75 Spike

Depositor

Marceline Côté

Article

Tauzin et al Cell Rep. 2023 Jan 31;42(1):111998. doi:

Insert

SARS-CoV-2 BA.2.75 Spike (S )

Use

Tags

Expression

Mammalian

Mutation

Contains the BA.2 mutations (T19I, LPPA24S, G142D…

Promoter

chicken β-actin promoter, CMV enhancer

Available since

Sept. 7, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pCAGGS SARS-CoV-2 BA.4/5 Spike

Plasmid

#186031

Purpose

Expresses codon-optimized full length SARS-CoV-2 BA.4/5 Spike

Depositor

Marceline Côté

Article

Tauzin et al Cell Rep. 2023 Jan 31;42(1):111998. doi:

Insert

SARS-CoV-2 BA.4/5 Spike (S )

Use

Tags

Expression

Mammalian

Mutation

BA.2 mutations (T19I, LPPA24S, G142D, V213G, G339…

Promoter

chicken β-actin promoter, CMV enhancer

Available since

June 8, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pCAGGS SARS-CoV-2 BQ.1.1 Spike

Plasmid

#193710

Purpose

Expresses codon-optimized full length SARS-CoV-2 BQ.1.1 Spike

Depositor

Marceline Côté

Article

Tauzin et al Cell Rep. 2023 Jan 31;42(1):111998. doi:

Insert

SARS-CoV-2 BQ.1.1 Spike (S )

Use

Tags

Expression

Mammalian

Mutation

Contains the following mutations: T19I, LPPA24S, …

Promoter

chicken β-actin promoter, CMV enhancer

Available since

Nov. 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pCAGGS SARS-CoV-2 BA.4.6 Spike

Plasmid

#190013

Purpose

Expresses codon-optimized full length SARS-CoV-2 BA.4.6 Spike

Depositor

Marceline Côté

Article

Tauzin et al Cell Rep. 2023 Jan 31;42(1):111998. doi:

Insert

SARS-CoV-2 BA.4.6 Spike (S )

Use

Tags

Expression

Mammalian

Mutation

Contains the following mutations: T19I, LPPA24S, …

Promoter

chicken β-actin promoter, CMV enhancer

Available since

Sept. 7, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA4/TO/GFP-GIGYF2

Plasmid

#141189

Purpose

Expresses GFP-GIGYF2 in mammalian cells, can be used to make inducible cell line

Depositor

Simon Bekker-Jensen

Article

Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

Insert

GIGYF2 (GIGYF2 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 5, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223-RIPK2

Plasmid

#23846

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

RIPK2 (RIPK2 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-RIPK1

Plasmid

#23442

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

RIPK1 (RIPK1 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pCIBN-hTRF1-tagRFP-T

Plasmid

#103811

Purpose

BLInCR 'Localizer' construct that marks telomeres and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-hTRF1-tagRFP-T (CIB1, TERF1 Human, Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag MKNK1

Plasmid

#20536

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

MKNK1 (MKNK1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pDONR223-TNNI3K

Plasmid

#23469

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TNNI3K (TNNI3K Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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cmamxA2-GFP

Plasmid

#107197

Purpose

Expresses human annexin A2-GFP fusion protein for live cell imaging, all type II Ca2+ binding sites deleted

Depositor

Volker Gerke, Ursula Rescher

Article

Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8

Insert

cmannexin A2 (ANXA2 Human)

Use

Tags

Green Fluorescent Protein

Expression

Mammalian

Mutation

all type II Ca2+ binding sites deleted ( D161A, E…

Promoter

CMV

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223-MKNK1

Plasmid

#23531

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MKNK1 (MKNK1 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-MKNK2

Plasmid

#23735

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MKNK2 (MKNK2 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pLD-puro-Cc-PARK7WT-VA

Plasmid

#115182

Purpose

Lentiviral transduction and expression of PARK7WT into any mammalian cell

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

PARK7WT (PARK7 Human)

Use

Lentiviral

Tags

Versatile affinity tag (2XStreptactin II-6XHis-3X…

Expression

Mutation

Promoter

CMV

Available since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

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