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Plasmid#33255DepositorInsertPIK3CA (PIK3CA Human)
UseRetroviralTagsHemagglutinin (HA)ExpressionMammalianMutationchanged histidine 1047 to arginine and phenylalan…PromoterSV40Available sinceJan. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-TfR20-SNAP-IRES-Puro
Plasmid#171018PurposeLentiviral expression of a transferrin receptor-SNAP-tag fusion in mammalian cellsDepositorInsertTransferrin receptor (Human) - SNAP-tag (TFRC Human)
UseLentiviralTagsSNAPtag and TfRExpressionMammalianMutationPromoterEF1Available sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Tight-Puro FLAG-SMAD4 TetON
Plasmid#107129PurposeInducible expression of Flag-tagged SMAD4 in mammalian cellsDepositorInsertSMAD4
UseLentiviralTagsFLAGExpressionMutationPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF2α P405A Δ450-572-pBabe-Puro
Plasmid#25958DepositorInsertHypoxia inducible factor 2 alpha (EPAS1 Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationcDNA changed amino acids: Proline 405 to Alanin…PromoterAvailable sinceSept. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLEX-uORF-HA-birA*-STOP-IRES-Puro
Plasmid#120558PurposeExpresses control HA-birA* in mammalian cells & for virus productionDepositorInsertbirA
UseLentiviralTagsHAExpressionMammalianMutationR118GPromoterCMVAvailable sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro U6 sgRNA BfuAI stuffer A-tract
Plasmid#138525PurposeExpresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt control A-tract repeat RNA sequence.DepositorInsertControl non-PRC2 binding A-tract repeat RNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
untargeted Aurora B FRET sensor Kif2 substrate
Plasmid#45215DepositorInsertuntargeted Aurora B FRET sensor
UseTagsCyPET, YPETExpressionMammalianMutationPromotert7Available sinceMay 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV Puro DEST (w118-1) FAM149A
Plasmid#107510PurposeExpressing FAM149a, puromycin resistantDepositorInsertFAM149A (FAM149A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-moxGFP-P2A-T2A-cMyc-Puro
Plasmid#176892PurposeSleeping Beauty transposon-based vector for mammalian polycistronic expression of moxGFP, c-Myc and an additional gene of interestDepositorInsertmoxGFP-P2A-T2A-c-Myc
UseTransposonTagsMyc and moxGFPExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
nonphosphorylatable Mis12-targeted Aurora B FRET sensor
Plasmid#45229DepositorInsertnon phosphorylatable Mis12-targeted Aurora B FRET sensor
UseTagsExpressionMammalianMutationT-A mutation in substrate sequencePromoterAvailable sinceJuly 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
MSCV-BRD9_BRD7BD-PGK-Puro-IRES-GFP
Plasmid#75118PurposeRetroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the BRD7 bromodomainDepositorInsertBRD9 (BRD9 Human)
UseRetroviralTagsExpressionMutationBromodomain replaced with the bromodomain of BRD7PromoterAvailable sinceMay 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
NSP13-1x-BioID2 pBabe-puro (SARS-CoV-2)
Plasmid#186007PurposeExpresses SARS-CoV-2 NSP13 fused to BioID2 and HA tagDepositorInsertSARS-CoV-2 NSP13 (ORF1ab SARS-CoV-2)
UseRetroviralTagsBioID2 and HAExpressionMammalianMutationPromoterLTRAvailable sinceJune 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH1
Plasmid#164800PurposeFor CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA1DepositorInsertsERH CRISPRi gRNA1
puro-T2A-BFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1Alpha and U6Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH2
Plasmid#164801PurposeFor CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA2DepositorInsertsERH CRISPRi gRNA2
puro-T2A-BFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1Alpha and U6Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
piggyBac_rtTA (4th_Gen)_Gateway_IRES_NGN2-2A-PURO_Synapsin-BirA. (pEHA1618)
Plasmid#209084PurposeNgn2 mediated cortical neuron induction, gateway destination, Synapsin promoter BirA ligase expressionDepositorInsertsUseTagsExpressionMammalianMutationhuman codon optimizedPromoterhSynAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro U6 sgRNA BfuAI stuffer G-tract
Plasmid#138524PurposeExpresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt PRC2-binding G-tract repeat RNA sequence.DepositorInsertPRC2-binding G-tract repeat RNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
Direct-seq_lentiGuide-Puro-tRNA-Tail-8A8G
Plasmid#157984PurposeExpresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.DepositorInsertS. pyogenes sgRNA cassette (tRNA-Tail-8A8G)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV Puro DEST (w118-1) EPB41L1
Plasmid#107508PurposeExpressing EPB41L1, puromycin resistantDepositorInsertEPB41L1 (EPB41L1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-C-Myc-DDK-IRES-Puro LARP7
Plasmid#200725PurposeLentiviral expression of Flag-Tagged human LARP7DepositorInsertLARP7 (LARP7 Human)
UseLentiviralTagsMyc-DDK from backbone plasmidExpressionMammalianMutationPromoterCMVAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Myc-PGK-Puro-IRES-GFP
Plasmid#75124PurposeRetroviral expression plasmid encoding wild type murine MycDepositorInsertmyelocytomatosis oncogene (Myc Mouse)
UseRetroviralTagsNoneExpressionMutationPromoterAvailable sinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only