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Showing: 5681 - 5720 of 148587 results

  3. SpySwitch

    Plasmid
    #184225
    Purpose
    Expresses SpySwitch in the bacterial cytoplasm. SpySwitch binds SpyTag-, SpyTag002- or SpyTag003-fusions non-covalently for affinity purification, allowing gentle pH or temperature release.
    Depositor
    Mark Howarth
    Article
    Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:
    Insert
    SpySwitch
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    E77A mutation prevents isopeptide bond formation,…
    Promoter
    T7
    Available since
    June 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    184225-plasmid-map-sequence-id-361604

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  5. pENTR-GFP-MDC1 (770-7)

    Plasmid
    #26284
    Depositor
    Eric Campeau
    Insert
    Mediator of DNA damage checkpoint 1 (MDC1 Human)
    Use
    Entry vector
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Available since
    Oct. 18, 2010
    Availability
    Academic Institutions and Nonprofits only
    26284-plasmid-map-sequence-id-12370

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  11. pDV052

    Plasmid
    #191825
    Purpose
    Plasmid for the transformation of the cyanobacterium Arthrospira platensis at the KmR locus. No promoter. No terminator. Golden Gate compatible. Selection: in A. platensis: KmR; E. coli: AmpR
    Depositor
    Jim Roberts
    Article
    Tabakh et al STAR Protoc. 2023 Feb 8;4(1):102087. doi
    Type
    Empty backbone
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    191825-plasmid-map-sequence-id-377967

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  14. piRFP

    Plasmid
    #31857
    Purpose
    a phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imaging
    Depositor
    Vladislav Verkhusha
    Article
    Filonov et al Nat Biotechnol. 2011 Jul 17. doi: 10.103
    Has Service
    Cloning Grade DNA
    Insert
    iRFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    T2A, S13L, A92T, V104I, V114I, E161K, Y193K, F198…
    Promoter
    CMV
    Available since
    Sept. 6, 2011
    Availability
    Academic Institutions and Nonprofits only
    31857-plasmid-map-sequence-id-396432

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  22. pX330-BsaIx2-DD-Cas9

    Plasmid
    #75380
    Purpose
    Expresses DD-SpCas9 in mammalian cells and has a cloning site for an sgRNA. The FKBP12 L106P destabilization domain allows for inducible stabilization of Cas9 through the addition of Shield-1
    Depositor
    Michele Calos
    Article
    Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv
    Insert
    DD-SpCas9
    Use
    CRISPR
    Tags
    FKBP12 L106P DD
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    75380-plasmid-map-sequence-id-192102

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  26. HaloTag-A2A-adenosine receptor

    Plasmid
    #66995
    Purpose
    A2A-adenosine receptor tagged with HaloTag on amino terminus
    Depositor
    Catherine Berlot
    Insert
    HaloTag-A2A-adenosine receptor (ADORA2A Human)
    Use
    Tags
    Human Growth hormone Signal Sequence- HaloTag
    Expression
    Mammalian
    Mutation
    The signal sequence from human growth hormone was…
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    66995-plasmid-map-sequence-id-115422

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  34. qTAG-N-Blast-3xFLAG

    Plasmid
    #207684
    Purpose
    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-3xFLAG cassette
    Depositor
    Laurence Pelletier
    Article
    Philip et al bioRxiv
    Type
    Empty backbone
    Use
    CRISPR; Donor cassette
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207684-plasmid-map-sequence-id-413749

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  38. pENN.AAV.tMCK.PI.eGFP.WPRE.bGH

    Plasmid
    #105556
    Purpose
    AAV expression of EGFP from tMCK promoter
    Depositor
    James M. Wilson
    Has Service
    AAV9
    Insert
    EGFP
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tMCK
    Available since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    105556-plasmid-map-sequence-id-202405

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  40. Villinpromoter-blue-FlpOERT2

    Plasmid
    #67278
    Purpose
    4hydroxytamoxifen inducible FlpO recombinase controlled by intestine specific promoter (Villin). FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon context
    Depositor
    Jannik Elverløv-Jakobsen
    Article
    Callesen et al Mol Oncol. 2017 Nov;11(11):1616-1629. do
    Insert
    mTQ2=mturquoise2 blue fluorescent gene
    Use
    Tags
    linked to FlpO through an P2A ribosomal skipping …
    Expression
    Mutation
    Promoter
    Villin
    Available since
    July 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    67278-plasmid-map-sequence-id-115887

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Showing: 5681 - 5720 of 148587 results