Addgene

Showing: 2841 - 2860 of 11869 results

Results per page:

Sort results by:

Ii-Str_ManII-SBP-mCherry

Plasmid

#65256

Purpose

synchronize trafficking of ManII from the ER

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ii-Streptavidin and truncated Mannosidase II

Use

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSFI-myr-akt-T308A/S473A

Plasmid

#49191

Purpose

cDNA of Akt1 with myristyolation signal and point mutation from T to A at position 308 and S to A at position 473 in cloning vector

Depositor

Peter Vogt

Article

Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

Insert

Akt1 (Akt1 Mouse)

Use

Adaptor plasmid

Tags

HA tag and myristolation signal

Expression

Mutation

T308A; S473A

Promoter

T7

Available since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAFNF-ECFP-Actb

Plasmid

#169787

Purpose

An actin FRET sensor. Must be expressed with Venus-actin for FRET.

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Actb (Actb Mouse)

Use

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
p3.5VPIII.EGFP.IGR2.1

Plasmid

#40865

Depositor

Harold Gainer

Article

Fields et al J Neurosci. 2003 Aug 27;23(21):7801-9.

Insert

Mouse vasopressin gene (Avp Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

AVP

Available since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 Hmga2 wt

Plasmid

#14789

Depositor

David Bartel

Article

Mayr et al Science. 2007 Mar 16. 315(5818):1576-9.

Insert

Hmga2 wt (Hmga2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 6, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-IP-EGFP-ULK1(K46N)

Plasmid

#38197

Depositor

Noboru Mizushima

Article

Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

Insert

a novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

changed Lysine 46 to Asparagine

Promoter

Available since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only
Hmga2 3'UTR m4 luciferase (Luc-m4)

Plasmid

#14787

Depositor

David Bartel

Article

Mayr et al Science. 2007 Mar 16. 315(5818):1576-9.

Insert

Luc-m4 (Hmga2 Mouse)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

m4 mutant (see article and author's map for …

Promoter

Available since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
GST scFv [N100/13]

Plasmid

#190521

Purpose

Mammalian Expression of GST scFV. Derived from hybridoma N100/13.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

GST (Schistosoma japonicum) recombinant scFV

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-VAPA [N479/22R]

Plasmid

#188181

Purpose

Mammalian Expression Plasmid of anti-VAPA (Rat). Derived from hybridoma N479/22

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-VAPA (Rattus norvegicus) recombinant mouse monoclonal antibody (Vapa Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-VAPA [N479/12R]

Plasmid

#199401

Purpose

Mammalian expression plasmid of Anti-VAPA [N479/12R] (Rat). Derived from hybridoma N479/12.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-VAPA (Rat) recombinant mouse monoclonal antibody (Vapa Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV5-Flag-mEPLIN alpha

Plasmid

#40955

Depositor

Scott Maul, Elizabeth Luna

Article

Maul et al Gene. 2001 Jan 10;262(1-2):155-60.

Insert

EPLIN alpha (Lima1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only
XE141 sFRP-1-CS2+

Plasmid

#16693

Depositor

Randall Moon

Insert

sFRP-1 (Sfrp1 Mouse)

Use

Xenopus expression

Tags

Expression

Mutation

Promoter

Available since

May 22, 2009

Availability

Academic Institutions and Nonprofits only
PKC epsilon DN

Plasmid

#21243

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC epsilon (Prkce Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

K437R

Promoter

Available since

Aug. 13, 2009

Availability

Academic Institutions and Nonprofits only
AAV_pMBP-dnVamp2-P2AT2A-EGFP-caax

Plasmid

#190153

Purpose

Expresses dominant-negative Vamp2 (mouse Vamp2 AA 1-94) plus membrane-targeted EGFP in oligodendrocytes; AAV vector

Depositor

Brad Zuchero

Article

Lam et al Nat Commun. 2022 Sep 23;13(1):5583. doi:

Insert

Vamp2 (Vamp2 Mouse)

Use

AAV

Tags

P2AT2A-EGFP-caax

Expression

Mammalian

Mutation

Truncation that includes only AA #1-94

Promoter

MBP

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pQCi-mB2m-sgRNA

Plasmid

#154090

Purpose

pQCi backbone with sgRNA targeting murine Beta-2-microglobulin

Depositor

Scott McComb

Article

Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

Insert

sgRNA targeting murine B2M locus

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
RSRF-Luc-2mut

Plasmid

#31819

Depositor

Astar Winoto

Article

Woronicz et al Mol Cell Biol. 1995 Nov;15(11):6364-76.

Insert

Mutant MEF2-dependent promoter (-307 to -242 of Nur77/NR4A1)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

two CTATATTTAG RSRF sites mutated to CGATATTTCG

Promoter

mutated MEF2-dependent (-307 to -242 of Nur77/NR4…

Available since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only
pHybr_r2a>mG2a-srt-his

Plasmid

#124803

Purpose

HDR template to knock-in mouse IgG2a Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG2a producing cell lines.

Depositor

Ferenc Scheeren, Martijn Verdoes

Article

van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

Insert

Murine IgG2a

Use

CRISPR; Hdr template

Tags

Histag (HHHHHH) and Sortag (LPETGG)

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC-MLXγ

Plasmid

#211755

Purpose

Cloning vector for the expression of mouse MLXγ

Depositor

Russell Goodman

Article

Singh et al Cell Metab. 2024 Jan 2;36(1):144-158.e7.

Insert

MLX (Mlx Mouse)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Neuroligin-3 GFP/pCMV

Plasmid

#197338

Purpose

Plasmid expressing an antigen targeting Neuroligin-3

Depositor

Thomas Südhof

Insert

Neuroligin3 (Nlgn3 Mouse)

Use

Tags

mVenus

Expression

Mammalian

Mutation

Promoter

Available since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3-HA-mMcl1

Plasmid

#25714

Depositor

Roger Davis

Article

Morel et al Mol Cell Biol. 2009 Jul . 29(14):3845-52

Insert

mMCL1 (Mcl1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only