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We narrowed to 5,218 results for: pCas

Showing: 2821 - 2840 of 5218 results

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nCas9-UGI-NLS-P2A-EGFP (pJUL1001)

Plasmid

#123611

Purpose

CAG promoter expression plasmid for hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (BE3 control without rAPOBEC1).

Depositor

Keith Joung

Article

Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

Insert

nCas9-UGI-NLS-P2A-EGFP

Use

Tags

Expression

Mammalian

Mutation

D10A in SpCas9

Promoter

CAG

Available since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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Click editor utilizing mSA for template recruitment - pCMV-T7-mSA-nCas9-EcKlenow (JO1391)

Plasmid

#217802

Purpose

Variant CE1 construct with mSA for template recruitment, expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

mSA-linker-SV40NLS-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

Promoter

CMV and T7

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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HUHe variant click editor (CE1) - pCMV-T7-StCV-nCas9-EcKlenow (JO1269)

Plasmid

#217790

Purpose

Variant CE1 construct with StCV HUH endonuclease, expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

StCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

Promoter

CMV and T7

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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HUHe variant click editor (CE1) - pCMV-T7-CaCV-nCas9-EcKlenow (JO1261)

Plasmid

#217791

Purpose

Variant CE1 construct with CaCV HUH endonuclease, expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

CaCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

Promoter

CMV and T7

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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HUHe variant click editor (CE1) - pCMV-T7-Chimp17-nCas9-EcKlenow (JO1275)

Plasmid

#217792

Purpose

Variant CE1 construct with Chim HUH endonuclease, expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

Chimp17-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

Promoter

CMV and T7

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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HUHe variant click editor (CE1) - pCMV-T7-GoCV-nCas9-EcKlenow (JO1277)

Plasmid

#217793

Purpose

Variant CE1 construct with GoCV HUH endonuclease, expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

GoCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

Promoter

CMV and T7

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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HUHe variant click editor (CE1) - pCMV-T7-SwCV-nCas9-EcKlenow (JO1270)

Plasmid

#217794

Purpose

Variant CE1 construct with SwCV HUH endonuclease, expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

SwCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

Promoter

CMV and T7

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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HUHe variant click editor (CE1) - pCMV-T7-RaCV-nCas9-EcKlenow (JO1268)

Plasmid

#217795

Purpose

Variant CE1 construct with RaCV HUH endonuclease, expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

RaCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

Promoter

CMV and T7

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-M-MLV(WT) (DRR1012)

Plasmid

#217799

Purpose

Variant CE1 construct with wild-type M-MLV revese transcriptase (RT), expressed from CMV or T7 promoters

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

PCV2-XTEN-nSpCas9-BPNLS-M-MLV-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

nSpCas9(H840A)

Promoter

CMV and T7

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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HUHe variant click editor (CE1) - pCMV-T7-BDFV-nCas9-EcKlenow (JO1271)

Plasmid

#217785

Purpose

Variant CE1 construct with BDFV HUH endonuclease, expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

BDFV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

Promoter

CMV and T7

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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HUHe variant click editor (CE1) - pCMV-T7-CoCV-nCas9-EcKlenow (JO1262)

Plasmid

#217786

Purpose

Variant CE1 construct with CoCV HUH endonuclease, expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

CoCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

Promoter

CMV and T7

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
HUHe variant click editor (CE1) - pCMV-T7-FiCV-nCas9-EcKlenow (JO1263)

Plasmid

#217787

Purpose

Variant CE1 construct with FiCV HUH endonuclease, expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

FiCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

Promoter

CMV and T7

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
HUHe variant click editor (CE1) - pCMV-T7-GuCV-nCas9-EcKlenow (JO1279)

Plasmid

#217788

Purpose

Variant CE1 construct with GuCV HUH endonuclease, expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

GuCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

Promoter

CMV and T7

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
HUHe variant click editor (CE1) - pCMV-T7-PCV1-nCas9-EcKlenow (JO1315)

Plasmid

#217789

Purpose

Variant CE1 construct with PCV1 HUH endonuclease, expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

PCV1-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

Promoter

CMV and T7

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pNOC_dCas9-KRAB_sgRNA Td2

Plasmid

#176264

Purpose

pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged to the KRAB domain and Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 2

Depositor

John van der Oost

Article

Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

Insert

dead spCas9

Use

CRISPR, Luciferase, and Synthetic Biology ; Expre…

Tags

Kruppel associated box domain and luciferase

Expression

Mutation

H840A and D10A mutations on spCas9 to inactivate …

Promoter

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-PEmax-P2A-hMLH1dn

Plasmid

#174828

Purpose

Mammalian expression of SpCas9 PEmax prime editor with P2A-human MLH1dn (codon optimized)

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

PEmax-P2A-hMLH1dn

Use

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Mutation

Detailed in manuscript; deletion of residues 754-…

Promoter

CMV

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-PEmax-P2A-BSD

Plasmid

#174821

Purpose

Mammalian expression of SpCas9 PEmax prime editor with P2A-BSD marker

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

PEmax-P2A-BSD

Use

Tags

P2A-BSD, SV40 bpNLS, and c-Myc NLS

Expression

Mammalian

Mutation

Detailed in manuscript

Promoter

CMV

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-PE2-P2A-hMLH1dn

Plasmid

#174827

Purpose

Mammalian expression of SpCas9 prime editor 2 with P2A-human MLH1dn (codon optimized)

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

PE2-P2A-hMLH1dn

Use

Tags

SV40 bpNLS

Expression

Mammalian

Mutation

Detailed in manuscript; deletion of residues 754-…

Promoter

CMV

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-ePhi29 (LM2993)

Plasmid

#208959

Purpose

A variant CE1 construct with ePhi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

PCV2-XTEN-nSpCas9-BPNLS-ePhi29-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

nSpCas9(H840A); ePhi29(-exo)(D169A;M8R/V51A/M97T/…

Promoter

CMV and T7

Available since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-M-MLV(PE2) (JO586)

Plasmid

#217800

Purpose

Variant CE1 construct with engineered M-MLV reverse transcriptase (RT; found in PE2), expressed from CMV or T7 promoters

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

PCV2-XTEN-nSpCas9-BPNLS-M-MLV(PE2)-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

nSpCas9(H840A); PE2 M-MLV(D200N/L603W/T330P/T306K…

Promoter

CMV and T7

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 2821 - 2840 of 5218 results

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